Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
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16.5K Views
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13:19 min
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November 2nd, 2013
DOI :
November 2nd, 2013
•Philippe Pérot1,2, Valérie Cheynet1,2, Myriam Decaussin-Petrucci1,3,4, Guy Oriol1,2, Nathalie Mugnier5, Claire Rodriguez-Lafrasse1,4,6, Alain Ruffion1,4,7, François Mallet1,2
1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department, BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 4Medical Faculty, Lyon 1 University, 5Data and Knowledge Laboratory, BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon
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Rozdziały w tym wideo
0:05
Title
2:42
Handling of Prostrate Tissues
4:03
RNA Extraction, Purification, and Quality Control
5:53
Target Preparation
7:33
Hybridization to the HERV Chip Microarray
9:24
Scanning and Data Analysis
10:14
Results: Identification of Active HERV Loci in Prostrate Tissues
11:49
Conclusion
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