JoVE Journal

Immunology and Infection

Masz pełny dostęp do tej treści za pośrednictwem

Nanyang Technological University

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Please note that all translations are automatically generated. Click here for the English version.

10.9K Views

•

11:28 min

•

October 7th, 2011

DOI :

October 7th, 2011

•

Xiaonan Dong¹, Pinghui Feng²

¹Center for Autophagy Research, Department of Internal Medicine, UT Southwestern Medical Center, ²Department of Microbiology, UT Southwestern Medical Center

Podsumowanie

We describe a protocol to identify key roles of host signaling molecules in lytic replication of a model herpesvirus, gamma herpesvirus 68 (γHV68). Utilizing genetically modified mouse strains and embryonic fibroblasts for γHV68 lytic replication, the protocol permits both phenotypic characterization and molecular interrogation of virus-host interactions in viral lytic replication.

Rozdziały w tym wideo

0:05

Title

2:07

Mouse Infection with γHV68

3:16

Preparation of Cell Lysate from Lung Tissue

4:32

Plaque Assay Procedure

6:41

Determine γHV68 Multi-step Growth Kinetics in Mouse Embryonic Fibroblasts

8:15

Molecular Dissection of γHV68 Lytic Replication in Mouse Embryonic Fibroblasts

9:28

Representative Results

10:58

Conclusion

Powiązane Filmy

article

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

10.4K Views

article

A Protocol for Analyzing Hepatitis C Virus Replication

24.0K Views

article

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

12.6K Views

article

Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways

8.4K Views

article

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

26.2K Views

article

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

28.1K Views

article

An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction

11.2K Views

article

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

12.3K Views

article

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

7.4K Views

article

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

13.1K Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej