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Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

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06:17 min

February 28th, 2025

DOI :

10.3791/67178-v

February 28th, 2025


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Covalent Fragment Screening

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Introduction

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Quantitative-Irreversible Tethering Assay for Electrophilic Ligand Screening

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CPM-Quenching to Quantify the Amount of Unreacted Thiol Remaining in the Protein and Glutathione Reaction Plates of Quantitative-Irreversible Tethering Assay

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Data Analysis and Hit Selection After the Quantitative-Irreversible Tethering Assay for Electrophilic Ligand Screening

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