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Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

3.1K Views

10:59 min

August 17th, 2022

DOI :

10.3791/64254-v

August 17th, 2022


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Single molecule FRET

Hoofdstukken in deze video

0:04

Introduction

1:04

Slide and Coverslip Cleaning

3:04

mGluR2 Expression with Incorporated Unnatural Amino Acid, Fluorescent Labeling, and Extraction

5:13

Single-Molecule Flow Chamber Assembly and Functionalization

6:23

Microscope Setup and smFRET Data Acquisition

7:47

Data Analysis

8:39

Results: Analyzing Conformational Dynamics of Membrane Receptors Proteins

10:11

Conclusion

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