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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.

Hoofdstukken in deze video

0:05

Introduction

0:53

Formaldehyde Fixation

7:54

Results: Capture Hi-C, 5C, and Hi-C Interaction Maps across the 3 Mb and 1 Mb Captured Region

9:05

Conclusion

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