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Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

2.3K Views

08:26 min

April 6th, 2022

DOI :

10.3791/63459-v

April 6th, 2022


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Fluorescent labeled Proteins

Hoofdstukken in deze video

0:04

Introduction

0:58

Kinetic Binding Experiments

2:13

Equilibrium Binding Experiments

2:45

Analysis of Flow Cytometry Data

5:13

Converting Fluorescence Intensity to the Mean Number of Binding Sites

7:05

Results: Specific Binding of Fluorescent-Labeled Coagulation Factor X or fX to Artificial Phospholipid Vesicles

7:42

Conclusion

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