Name: low-intensity blast wave model for preclinical assessment of closed-head mild traumatic brain injury in rodents
Uploaded: 2020-11-06T13:00:00.000+00:00
Duration: 6 min 9 s
Description: Watch this Scientific Journal Video about Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents at JoVE.com

We thank R. Gettens, N. St. Johns, P. Bennet and J. Robson for their contributions to the development of the TBI model. NARSAD Young Investigator Grants from the Brain &#38; Behavior Research Foundation (F.P. and M.J.R.), a Research Grant from the Darrell K. Royal Research Fund for Alzheimer&#8217;s Disease (F.P.) and a PhRMA Foundation Award (M.J.R.) supported this research. This work was supported through pre-doctoral fellowships from the American Foundation for Pharmaceutical Education (A.F.L and B.P.L.).

The protocol follows the animal care guidelines of the University of Cincinnati and West Virginia University. All procedures involving animals were approved by the Institutional Animal Care and Use Committees (IACUC), and were performed according to the principles of the Guide for the Care and Use of Laboratory Animals.
1. Installation of the blast TBI setup
<ol>
	<li>Acquire all the working parts that are required for the setup, including: shock tube consisting of steel driven- and driver section, polyester membrane, securing bolts, pressure sensors, polyvinyl chloride (PVC) pipe shield to protect peripheral organs, 9.53 mm high pressure hydraulic line and quick connect male and female attachments, high flow gas regulator and a gas cylinder with wall-mount bracket (see Figure 1A,B and Table of Materials). 
	NOTE: The specifications of the driven and driver section used here (see Figure 2 and Table of Materials) have been established to produce a consistent short-duration scaled blast wave (see Figure 3C,D) to induce mild to moderate TBI in mice. For this purpose, a taper-designed (6&#176; taper) short driver section was selected. The length and diameter of the driven and driver sections can be modified to specifically research blast wave29,30,31,32, compression wave18, or shock wave dynamics33. For experiments with rats, the dimensions of the shock tube need to be adapted to yield comparable forces according to retain pertinent body scaling parameters17 (see Table of Materials).</li>
	<li>Install the individual working parts of the setup on machine slide tables that are fixed on a stable, easy to clean surface (preferably stainless steel for use in rodents) in laboratory space approved for animal experiments. 
	NOTE: The blast wave experiments produce a considerable level of noise; therefore choose a location within sound absorbing laboratory space, where noise will not interfere with other experiments/laboratory groups.
	<ol>
		<li>Fix the PVC pipe shield perpendicular to the shock tube setup so that the body of the rodent will be fully covered and only the head protrudes. 
		NOTE: For the standard procedure to induce mild to moderate TBI described here, the center of the head is located 5 cm from the end of the driven section for mice.</li>
		<li>Wall mount gas cylinder in close proximity to setup in accordance with OSHA and all other pertinent safety regulations. 
		NOTE: Compressed air, helium or nitrogen gas are commonly used to generate the blast waves in rodent shock tube models. All data presented here has been generated using helium, as this gas produces higher overpressure over a shorter duration34, allowing for appropriate scaling for murine subjects.</li>
	</ol>
	</li>
</ol>
2. Evaluation of the setup and blast wave properties using pressure sensor recordings.
<ol>
	<li>Prepare the shock tube.
	<ol>
		<li>Carefully cut the polyester membrane without bending and producing fissures, in order to ensure consistent rupture.</li>
		<li>Insert the membrane between the driven and driver sections. Secure the sections by tightening connecting bolts.</li>
		<li>Check that the system is airtight and the membrane is tightly fixed between driver and driven sections.</li>
		<li>Connect the gas tank via a 9.53 mm high pressure hydraulic hose and quick connect attachments to the shock tube 
		NOTE: Driver and driven sections are machined to precise tolerances in order to afford a complete seal of membrane between sections. This allows for no gas leakage and precludes the use of any form of gasket/o-ring material and allows for greater consistency in generated waveform.</li>
	</ol>
	</li>
	<li>Install the pressure sensors for monitoring the blast waves (see Figure 1C).
	<ol>
		<li>Position one pressure sensor in the head placement area, and three sensors at the exit of the shock tube (see Figure 1C and 2).</li>
		<li>Initiate recording from pressure sensors, just prior to blast wave execution. Record the pressure wave data at 500,000 frames per second using a sensor signal conditioner and data acquisition board (see Table of Materials). 
		NOTE: Wear OHSA-approved earmuffs to ensure adequate hearing protection.</li>
		<li>Open the main valve of the compressed gas tank fully to allow the gas flow to produce a sudden, rapid pressure spike. 
		NOTE: The gas overpressure ruptures the polyester membrane to release a shock wave that transitions into a compression wave within the driven section and exits the tube in the direction of the head placement area.</li>
		<li>Turn off the gas flow immediately after the procedure. 
		NOTE: The setup can be equipped with a spring return valve to automatically and rapidly stop the gas flow.</li>
		<li>Analyze the pressure wave recordings using custom written computer program to determine peak overpressure and graph data. Data can be graphed with each sensor individually or overlaid on one another to demonstrate planarity of the wave generated (see Figure 3C,D). 
		NOTE: The analysis can technically be done using more readily available software, but due to the large data sets, these programs have long delays in generating plots.</li>
	</ol>
	</li>
	<li>Establish experimental conditions that are adequate for the aim of the designated TBI study, and confirm that the model produces a consistent blast wave with a peak over-pressure, duration, and impulse measurement comparable to a Friedlander wave (see Figure 3). Verify these parameters using the aforementioned computer software.
	<ol>
		<li>Calibrate the setup by repeating steps 2.1.1. to 2.2.5. and use the pressure wave recordings to determine whether the setup needs adjustment (for representative data see Figure 3).</li>
		<li>Modify the setup (if needed). 
		NOTE: The blast wave properties can be adjusted by minor modifications of the setup. For example, the distance of the head to the end of the driven section impacts the blast wave force at the level of the head. The thickness of the polyester membrane determines the level of peak overpressure, with thicker membranes increasing peak levels (see Figure 3A,B). Additionally, the setup allows selecting the direction by which the blast wave strikes/penetrates the head (i.e., head-on, side, top or underneath) and therefore different aspects can be investigated, such as blast wave injury alone or in combination with coup and contrecoup injury due to rapid rotational forces.</li>
		<li>Repeat steps 2.1.1 to 2.2.4 to establish desired blast wave properties (if needed) and control for reproducibility.</li>
		<li>Repeat steps 2.1.1 to 2.2.4 with polyester membranes of different thickness to evaluate scalability of the setup (for representative data see Figure 3A,B).</li>
	</ol>
	</li>
</ol>
3. Preparation of experimental setup and induction of mild TBI in rodents
NOTE: Transfer rodents to holding area 30 min to 1 h prior to start of TBI experiments to acclimatize. Select holding area that is minimally affected by noise of the procedure.
<ol>
	<li>Prepare all materials required for the experiment and check setup for proper installation (e.g., adjust parameters according to aim of study) (~5 &#8211; 10 minutes). 
	NOTE: Injury severity can be adjusted by selecting the thickness of the polyester membrane. Based on our studies, a membrane thickness of 25.4 to 102 &#956;m is utilized for mild to moderate TBI in mice35. We have previously utilized membranes with a thickness of 76.2 to 127 &#956;m to produce mild to moderate TBI in rats19.
	<ol>
		<li>Carefully cut the polyester membrane, insert it between the driven and driver sections and secure by tightening the connecting bolts.</li>
		<li>Connect the gas tank to the shock tube through the use of quick-release fittings. Ensure that the membrane is tightly fixed between driver and driven sections.</li>
		<li>Place three pressure sensors at the exit of the shock tube, 120&#176; apart, to monitor the blast wave properties during the TBI induction as described in step 2.2.2 and 2.2.5.</li>
		<li>Ensure distance from end of shock tube apparatus is correct for each respective subject using installed micrometer. Keep the positioning of the rodent&#8217;s head (i.e., position, distance) constant within studies to allow for consistent injury evaluation. 
		NOTE: As stated in 1.2.1., different types of injury can be induced by selecting the direction, in which the blast wave impacts the head. For the procedure to induce mild to moderate TBI described here, the body is placed perpendicular to the shock tube that the blast wave impacts the side of the head. In this setting, the head is allowed free mobility and hence is exposed to the blast wave and rapid rotational forces allowing the generation of coup and contrecoup effects.</li>
		<li>Initiate the recording from the pressure sensors using the graphical user interface (GUI) of the software.</li>
	</ol>
	</li>
	<li>Anesthesia and positioning of rodents in setup
	<ol>
		<li>Transfer rodents from holding room and induce anesthesia with 4% isoflurane in oxygen and maintain with 2% isoflurane in oxygen to reduce distress and pain. 
		NOTE: Make sure the animal is non-responsive to toe or tail pinch prior to proceeding. Make sure the induction of anesthesia is consistent for all experimental animals, including sham controls. This procedure requires a low-level and short duration of anesthesia.</li>
		<li>Place the fully anesthetized rodent in the PVC pipe shield with cushioning to protect peripheral organs from the blast wave. 
		NOTE: Control subjects are anesthetized and placed in proximity to the setup, but are not directly subjected to the blast wave. Ensure that the controls are subjected to the noise generated by the shock tube.</li>
		<li>Place the head of the rodent within the head placement area and support it from below, either by a support built directly into the shielding apparatus or a gauze pad. Determine the head alignment according to each individual rodent&#8217;s anatomy, with the occipital condyle aligned with the edge of the protective shielding. 
		NOTE: Avoid directing the pressure wave directly towards the brainstem to decrease mortality. Injury to the respiratory center of the brainstem and the cervical spinal cord is known to contribute to breathing abnormalities and even death in rodent models of TBI36,37,38.</li>
	</ol>
	</li>
	<li>Exposure of rodents to blast wave.
	<ol>
		<li>Rapidly open the main valve of the compressed gas tank to produce a pressure spike that ruptures the membrane and produce a loud explosion that confirms the generation of a pressure wave. The membrane will be visually ruptured when removed after the experiment. 
		NOTE: A high-speed camera can be used to capture the coup and contrecoup effects of the rotational acceleration experienced by the rodent for further analysis.</li>
		<li>Turn off the gas flow immediately after hearing the explosion.</li>
	</ol>
	</li>
	<li>Recovery from blast wave exposure
	<ol>
		<li>After blast wave exposure, remove the rodent from the apparatus and place on a flat surface directly adjacent to the shock tube on their side.</li>
		<li>Monitor subjects to determine righting reflex time (RRT). Use a stopwatch to record time from blast wave exposure until they regain inherent righting reflex. (see Figure 4A).</li>
		<li>As soon as subjects regain their righting reflex, place them in their respective home cage where they are monitored for adverse reactions (i.e., seizures, difficulty breathing, bleeding from a bodily orifice) for the next 24 h.</li>
		<li>After the initial monitoring period, subjects can be analyzed using various biochemical, neuropathological, neurophysiological, and behavioral assays of researcher&#8217;s choosing (see below).</li>
	</ol>
	</li>
	<li>Prepare setup and room for next experiment.
	<ol>
		<li>Clean setup with detergent to remove odor.</li>
	</ol>
	</li>
</ol>
4. Downstream applications for rodents exposed to blast wave/rotational forces and controls
NOTE: In previous studies, the effects of mild to moderate TBI at various time points after exposure to a blast wave and rotational forces were assessed in rodents using downstream applications, including biochemical, neuropathological, neurophysiological, and behavioral analyses19.
<ol>
	<li>Biochemical analysis
	<ol>
		<li>At defined experimental time points (hours to days post-mild TBI), harvest tissue (e.g., brain, blood) for biochemical analysis using standard protocols as described19.</li>
		<li>Use tissue for biochemical analysis (i.e., immunoblotting, ELISA, etc.) to assess the effect of mild TBI on neurobiological and pathophysiological processes.</li>
	</ol>
	</li>
	<li>Neuropathological analysis
	<ol>
		<li>At defined experimental time points (hours to days post-mild TBI), perfuse rodents transcardially with saline solution followed by 4% paraformaldehyde solution to fix tissue as described19. 
		NOTE: Some applications are not compatible with paraformaldehyde fixation (e.g., silver staining, some antibodies for immunohistochemistry).</li>
		<li>Use perfused, fixed tissue for anatomical, histological and molecular analyses to assess neuropathological changes associated with mild TBI, including neuroinflammation, neurodegeneration, and neurochemical changes as described19.</li>
	</ol>
	</li>
	<li>Neurophysiological analysis in brain slices
	<ol>
		<li>At defined experimental time points (hours to days post-mild TBI), sacrifice rodents by decapitation, remove brain and prepare brain slices as described19.</li>
		<li>Perform electrophysiological recordings as described19 to assess the effect of mild TBI on basal synaptic properties and synaptic plasticity.</li>
	</ol>
	</li>
	<li>Behavioral analysis
	<ol>
		<li>At defined experimental time points (hours to days post-mild TBI), evaluate behavioral performance, including motor function (e.g., open field, rotarod, locomotor activity; see Figure 4D) and learning &#38; memory (e.g., fear conditioning, Barnes maze, Morris water maze).</li>
	</ol>
	</li>
</ol>

The authors declare that they have no competing interests.

We present here a preclinical mild TBI model that is cost-effective, easy to set up and execute, and allows for high-throughput, reliable, and reproducible experimental outcomes. This model provides protective shielding to peripheral organs to allow for focused investigation into mild TBI mechanisms while limiting the confounding variables of systemic injury. In contrast, other blast models are known to inflict damage to peripheral organs2,39,40. Another advantage of this model is its capability to deliver the blast wave from any desired angle compared to the fixed position in other blast models40. This allows for focused anatomical studies to better understand brain vulnerability.
In order to study human blast-related TBI, a relevant model for TBI should produce biomechanical forces comparable to those experienced by subjects during TBI induction. A clinically relevant model should also induce neurobiological, pathophysiological and behavioral outcomes observed in subjects suffering from mild TBI. In previous studies, the blast wave model presented here has been thoroughly examined17,19,21, and numerous biophysical and neurobiological aspects reminiscent of human TBI, including blast wave dynamics and forces, neuroinflammation, axonal injury and microvascular damage have been evaluated. These studies have provided evidence that this preclinical blast wave model for TBI produces reliable and reproducible neurobiological and pathophysiological changes associated with clinical TBI.
Furthermore, with the increased incidence of mild blast TBI within the military population7,8, this versatile rodent model for mild human TBI provides researchers with a valuable tool to investigate processes underlying blast-related TBI and explore novel therapeutic strategies. For example, our model demonstrates neurovascular complications, and highlights the importance for vascular intervention as a promising therapeutic approach22,23,35. Consistently, other preclinical models of blast TBI have also produced neurovascular effects associated with neurodegeneration and behavioral deficits2,25,40,41,42,43.
Based on previous research19,21,22,23, we have established that the blast wave model presented here may be well suited for the investigation into the pathophysiology and etiology of human concussion. Most preclinical TBI models do not permit head movement44 even though the biomechanical properties associated with rapid head acceleration/deceleration are a predictive factor for the development of a concussion in humans45,46. Consistent with the model described herein, Goldstein and colleagues14 showed that rapid head movement induced by blast forces are a prerequisite for the induction of behavioral deficits, possibly due to rotational forces and shearing. A better understanding of the pathophysiological changes that occur in mild TBI and in response to concussion would also help to determine clinical biomarkers and identify novel targets for the development of treatments for TBI.
Little is known about the pathophysiological changes and the disease progression following repetitive mild TBI (e.g., repetitive concussion experienced in sports). This preclinical model permits the study of repetitive mild TBI with little to no mortality. In contrast, some TBI models inflict severe injuries, and therefore it is often difficult, or inhumane, to induce further injury. In addition, severe injuries are often irreparable and the detection of subtle physiological changes may be precluded. This model also allows for the scalable investigation of various inter-injury intervals; a critical parameter for repetitive mild TBI that requires further characterization. After TBI, a CNS injury response is triggered that helps to protect brain integrity and prevent widespread neuronal cell death. The injury response may be, indeed, significantly impacted by the induction of another injury within a short time point after the initial injury. This model permits the investigation of the inter-injury interval, which is an important aspect of clinical trial design for repetitive mild TBI. Moreover, this scalable model allows for a rapid high-throughput workflow, which facilitates investigation of multiple parameters simultaneously, as well as the evaluation of therapeutic activity of novel interventions.
One limitation of this model is the inability to control the properties of the blast wave between the tube exit and the animal&#8217;s head. Although the blast wave is turbulent upon exit from the shock tube, the outcome measures are still reliable and reproducible with a consistent positioning of the rodent&#8217;s head18. Therefore, it is important to keep the experimental settings (i.e., head position and distance from shock tube exit) constant between all studies. In order to optimize model design and protocol, waveform dynamics between the tube exit and the head placement area have been measured (Figure 3) and modeled using numerical simulations18. Future projects will integrate finite element modeling to determine how force dynamics transfer from the skull to meninges, to cerebrospinal fluid, and finally into the brain tissue. The complex interplay of force dynamics and biophysics and resulting physiological responses are important areas in TBI research that have been so far underexplored.
In summary, we present here a protocol and visualized experiment of a blast wave injury model that has been developed to investigate the effects of mild TBI. The collective experience of engineers, physicians, and biomedical scientists contributed to the optimization of its biophysical/physiological validity and neurobiological relevance. This model has been thoroughly validated and has already produced meaningful results, especially in understanding early dynamics of mild TBI17,19,20,21,22,23. Exploiting this preclinical model to further study mild TBI will significantly advance our understanding of the pathophysiology and etiology of TBI and contribute to the development of novel interventions for the benefit of patients suffering from TBI.

Traumatic brain injury (TBI) accounts for more than two million hospital visits each year in the United States alone. Mild TBI commonly resulting from car accidents, sporting events, or falls represent approximately 80% of all TBI cases1. Mild TBI is considered the &#8216;silent disease&#8217; as patients often experience no overt symptoms in the days and months following the initial insult, but can develop serious TBI-related complications later in life2. Moreover, blast-induced mild TBI is prevalent among military service-members, and has been associated with chronic CNS dysfunction3,4,5,6. Due to the rising incidence of blast-related mild TBI7,8, preclinical modeling of neurobiological and pathophysiological processes associated with mild TBI has thus become a focus in the development of novel therapeutic interventions for TBI.
Historically, TBI research has primarily focused on severe forms of neurotrauma, despite the relatively lower number of severe human TBI cases. Preclinical rodent models for severe human TBI have been developed, including the controlled cortical impact (CCI)9,10 and fluid percussion injury (FPI)11 models, which are both well established to produce reliable pathophysiological effects12,13. These models have laid the groundwork for what is known today about neuroinflammation, neurodegeneration, and neuronal repair in TBI. Although considerable knowledge of the pathophysiology of TBI has been developed, there are currently no effective, FDA-approved treatments available for TBI.
More recently, the focus of TBI research has been broadened to include a wider spectrum of TBI-related pathologies with the ultimate goal of developing effective therapeutic interventions. Nevertheless, few preclinical models for mild TBI have been established that have shown measurable effects, and only a small number of studies have investigated the mild TBI spectrum2,14,15. As mild TBI accounts for the large majority of all TBI cases, reliable models of mild TBI are urgently needed to facilitate research into the etiology and neuropathophysiology of the human condition, in order to develop novel therapeutic strategies.
In conjunction with biomedical engineers and aerospace physicists, we have established a scalable, closed-head blast wave model for mild to moderate TBI. This preclinical rodent model has been specifically developed to investigate the effects of force dynamics, including blast waves and acceleration/deceleration movement, that are associated with human mild TBI obtained in military combat, sporting events, car accidents, and falls. As blast waves correlate with the force dynamics that cause mild TBI in humans, this model was designed to produce a consistent Friedlander waveform with an impulse, which is measured as pounds per square inch (psi)*millisecond (ms). The impulse level is scaled to fall below defined lung lethality curves for mice and rats in order to conduct preclinical investigations16,17,18. In addition, this model allows for investigation of coup and contrecoup injury due to rapid rotational forces of the animal&#8217;s head. This kind of injury is inherent to several types of clinical TBI presentations, including those observed in both military and civilian populations. Therefore, this versatile model fits a need that encompasses multiple clinical presentations of TBI.
The preclinical model presented here produces reliable and reproducible pathophysiological changes associated with clinical mild TBI as demonstrated by a number of prior studies17,19,20,21,22,23. Studies with this model showed that rats subjected to a low-intensity blast wave exhibited neuroinflammation, axonal injury, microvascular damage, biochemical changes related to neuronal injury and deficits in short-term plasticity and synaptic excitability19. However, this mild TBI model did not induce any macroscopic neuropathological changes, including tissue damage, hemorrhage, hematoma and contusion19 that have been commonly observed in studies using moderate to severe invasive TBI models10,24. Previous research19,21,22,23 has shown that this preclinical model can be used to characterize neurobiological and pathophysiological processes underlying the etiology of mild and moderate TBI17,19,20,21,22,23. This model also permits for testing of new therapeutic compounds and strategies, as well as the identification of novel, suitable targets for the development of effective TBI interventions19,21,22,23.
This model was developed to investigate effects induced by blast waves as well as rapid rotational forces on molecular, cellular and behavioral outcomes in rodents. Analogous to the blast wave model presented here, a number of preclinical models has been developed that attempt to recapitulate mild to moderate TBI using gas-driven overpressure waves2,14,17,25,26,27,28. Some of the limitations of other models include: the animal is fixed to a wire-mesh gurney and the head is immobilized upon impact; the peripheral organs are exposed to the wave in addition to the brain, which creates the confounding variables of polytrauma; and the models are large and stationary, which limits changing and adapting critical parameters to better model conditions reminiscent of human TBI.
The benefits of this bench-top, gas-driven shock tube setup are its relative low-cost for acquisition and running expenses, as well as ease of installation and use. Furthermore, the setup allows for high-throughput operation and generation of controlled reproducible blast waves and in vivo outcomes in both mice and rats. In order to control for consistent test conditions (i.e., constant blast wave and overpressure) the setup is equipped with pressure sensors. The advantages of this model for TBI include scalability of the injury severity and that mild TBI is induced using a non-invasive, closed-head procedure. Peak overpressure and subsequent brain injury increase with thicker polyester membranes in a consistent scalable manner17. The ability to scale TBI severity through membrane thickness is a useful tool to determine the level, at which specific outcome measures (e.g., neuroinflammation) become evident. Providing protective shielding for the peripheral organs, also allows focused investigation into mild TBI mechanisms by avoiding or reducing confounding variables of systemic injury, such as lung- or thoracic injury. Moreover, this setup allows selecting the direction, by which the blast wave strikes/penetrates the head (i.e., head-on, side, top or underneath) and therefore different types of TBI-inducing insults can be investigated. The standard procedure to induce mild to moderate TBI described here employs side exposure to evaluate the effects of blast wave injury in combination with coup and contrecoup injury due to rapid rotational forces. Furthermore, in order to investigate exclusively blast-induced injury, top down blast wave exposure can be employed in this model.

<table><tbody><tr><td>3/8 SAE High Pressure Hydraulic Hose</td><td>Eaton Aeroquip</td><td>R2-6-6-36M</td><td>Available from Grainger</td></tr><tr><td>3/8&#039;&#039; Quick Connect Female Plugs</td><td>Karcher</td><td>KAR 86410440</td><td></td></tr><tr><td>3/8&#039;&#039; Quick Connect Male Plugs</td><td>Karcher</td><td>KAR 86410440</td><td></td></tr><tr><td>ANY-maze video tracking software</td><td>Stoelting Co.</td><td>ANY-maze software</td><td></td></tr><tr><td>Clear Mylar membrane</td><td>ePlastics.com</td><td>POLYCLR0.003</td><td>http://www.eplastics.com/Plastic/Clear_Polyester_Film/POLYCLR0-003; Clear Mylar membrane is sold in various thicknesses. All are sold by vendor listed above.</td></tr><tr><td>Compound Slide Table (X2)</td><td>Grizzly Industrial</td><td>G5757</td><td></td></tr><tr><td>Deadman Gas Control Ball Valve</td><td>Coneraco Inc.</td><td>71-502-01</td><td>&quot;Apollo&quot;, Available from Grainger</td></tr><tr><td>Driver and driven section (murine)</td><td>own design/production</td><td>n/a</td><td>For further information please contact the authors</td></tr><tr><td>Driver and driven section (rat)</td><td>own design/production</td><td>n/a</td><td>For further information please contact the authors</td></tr><tr><td>Ear Muffs</td><td>3M</td><td>37274</td><td>Available from Grainger</td></tr><tr><td>Gas Regulator - Hi Flow 3500-600-580</td><td>Harris</td><td>3003539</td><td></td></tr><tr><td>Helium Gas</td><td>AirGas</td><td>HE 300</td><td>Tanks are available in various sizes</td></tr><tr><td>Inhalation Anesthesia System</td><td>VetEquip</td><td>901806</td><td></td></tr><tr><td>Input Module</td><td>National Instruments</td><td>NI 9223</td><td></td></tr><tr><td>Isoflurane</td><td>Baxter</td><td>NDC 10019-360-40</td><td>Ordered by veterinarian</td></tr><tr><td>Laboratory Timer/Stopwatch</td><td>Fisher Scientific</td><td>50-550-352</td><td></td></tr><tr><td>Labview version 12.0</td><td>National Instruments</td><td></td><td>Data Acquistion Software</td></tr><tr><td>Magnetic Dial Indicator/Micrometer</td><td>Grizzly Industrial</td><td>G9849</td><td></td></tr><tr><td>MATLAB</td><td>MathWorks</td><td></td><td>Software for pressure recording analysis</td></tr><tr><td>Oxygen Regulator</td><td>Medline</td><td>HCS8725M</td><td></td></tr><tr><td>PC for Data Processing</td><td>Dell</td><td></td><td></td></tr><tr><td>Polyvinylchloride Tubing - 25.4 mm</td><td>FORMUFIT</td><td>P001FGP-WH-40x3</td><td></td></tr><tr><td>Pressure sensors</td><td>PCB Piezotronics</td><td>102A05</td><td></td></tr><tr><td>Receiver USB Chassis</td><td>National Instruments</td><td>DAQ-9171</td><td></td></tr><tr><td>Sensor Signal Conditioner</td><td>PCB Piezotronics</td><td>482C series</td><td></td></tr><tr><td>Stainless NSF-Rated Mounting Table</td><td>Gridmann</td><td>GR06-WT2448</td><td></td></tr><tr><td>T Handle Allen Wrench - 3/16&#039;&#039;</td><td>S&amp;amp;K</td><td>73310</td><td></td></tr></tbody></table>

low-intensity blast wave model for preclinical assessment of closed-head mild traumatic brain injury in rodents

Traumatic brain injury (TBI) is a large-scale public health problem. Mild TBI is the most prevalent form of neurotrauma and accounts for a large number of medical visits in the United States. There are currently no FDA-approved treatments available for TBI. The increased incidence of military-related, blast-induced TBI further accentuates the urgent need for effective TBI treatments. Therefore, new preclinical TBI animal models that recapitulate aspects of human blast-related TBI will greatly advance the research efforts into the neurobiological and pathophysiological processes underlying mild to moderate TBI as well as the development of novel therapeutic strategies for TBI.
Here we present a reliable, reproducible model for the investigation of the molecular, cellular, and behavioral effects of mild to moderate blast-induced TBI. We describe a step-by-step protocol for closed-head, blast-induced mild TBI in rodents using a bench-top setup consisting of a gas-driven shock tube equipped with piezoelectric pressure sensors to ensure consistent test conditions. The benefits of the setup that we have established are its relative low-cost, ease of installation, ease of use and high-throughput capacity. Further advantages of this non-invasive TBI model include the scalability of the blast peak overpressure and the generation of controlled reproducible outcomes. The reproducibility and relevance of this TBI model has been evaluated in a number of downstream applications, including neurobiological, neuropathological, neurophysiological and behavioral analyses, supporting the use of this model for the characterization of processes underlying the etiology of mild to moderate TBI.

The scalability of the blast wave setup was tested using three different membrane thicknesses, 25.4, 50.8 and 76.2 &#956;m. Peak pressure levels were assessed at the head placement area and the exit of the shock tube apparatus using piezoelectric pressure sensors (see Figure 1 &#38; Figure 2). Peak pressures increase in concordance with membrane thickness at both sensor locations (Figure 3A,B), demonstrating that the peak pressure is scalable in nature. This property of the setup can be exploited to calibrate the system and assess its scalability as described in step 2.3.
In order to evaluate effects of blast-induced TBI in vivo, adult, 3-month-old, male, wild-type C57Bl/6J mice were exposed to blast waves produced by this setup (Figure 1 &#38; Figure 2) using the protocol described here. First, the effects of blast waves produced with two different membrane thicknesses (50.8 and 76.2 &#956;m) or sham treatment on righting reflex time (RRT) were assessed (Figure 4A). The latency of the mice to fully right themselves (4 paws on the ground) after anesthesia is determined here as RRT. The mice were anesthetized using isoflurane (consistent, short and mild anesthesia) and then underwent TBI induction or sham treatment. Immediately following injury, mice were allowed to recover and time to regain righting reflex was recorded. Mice that were exposed to a blast wave produced with the 76.2 &#956;m membrane exhibited a significant increase in RRT as compared to sham controls that underwent the same anesthesia procedure (Figure 4A), suggesting that this blast wave induces loss of consciousness. In contrast, mice exposed to a blast wave from the 50.8 &#956;m membrane exhibit no significant increases in RRT (Figure 4A), indicative of mild form of TBI. Rupture of a standard 76.2 &#956;m polyester membrane results in the rapid generation of a short duration blast wave of approximately 160 psi of overpressure (Figure 3C), which the left side of the subject&#8217;s cranium is exposed to during the experimental procedure.
The short-term physiological effects occurring after the exposure to blast wave and rotational forces in rodents are currently not well characterized. To delineate the acute effects of blast wave exposure and rotational forces from this model, we assessed core body temperature regulation and body weight. The temperature and body weight of adult, 3-month-old, male wild-type C57Bl/6J mice were recorded following TBI induction. Baseline core body temperature and body weight were recorded in the mice prior to TBI procedure or sham treatment. Exposure to a blast wave produced with the 76.2 &#956;m membrane significantly decreased the body temperature during the first hour in TBI-induced mice as compared to their sham controls (Figure 4B), indicative of a significant physiologic effect produced by TBI induction. Consistently, mice subjected to TBI using 76.2 &#181;m membranes exhibited an acute, time dependent yet significant reduction in total body weight one-day post-TBI compared to sham (Figure 4C).
In order to examine the impact of TBI on behavioral outcomes, the effect of blast-induced TBI on acute locomotor activity was analyzed (Figure 4D). Adult, 3-month-old, male C57Bl/6J mice underwent TBI induction using 76.2 &#181;m membrane or sham treatment and locomotor activity was monitored for 30 minutes three hours post-TBI. Exposure to a blast wave produced with the 76.2 &#956;m membrane resulted in an acute, significant decrease in locomotor activity (Figure 4D).
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/61244/61244fig01.jpg" /> 
Figure 1: Setup of murine blast wave model.&#160;(A-C) Representative images of the setup of the blast wave model for mice. Side view of the setup (A). Top view of the setup (B). 1, gas cylinder with a high flow gas regulator; 2, 9.53 mm high pressure hydraulic line and quick connect male and female attachments; 3, driver section of the shock tube; 4, driven section of the shock tube; 5, PVC pipe shield; 6, head placement area; 7, polyester membrane. The individual parts of the setup are installed on machine slide tables allowing for precise positioning of driver (3) and driven sections (4) in relation to subject undergoing injury induction. (C) Top view of setup with pressure sensor placements. Three sensors are located in one plane at the exit of the shock tube, 120 degrees apart (S1 - S3), to monitor the blast wave properties during the TBI induction. One sensor is installed at the head placement area (S4). <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/61244/61244fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/61244/61244fig02.jpg" /> 
Figure 2: Schematic of murine overpressure shock tube.&#160;Precision-machined shock tube is made from high-tensile steel. Internal space of the driver section is angled at 6 degrees. Internal diameter of driver and driven section is 37 mm. Mating surfaces of driver-driven sections are precision-machined to ensure complete seal. The entire shock tube is industrially clamped to a machine slide table to ensure solid mounting and consistency of blast wave generation. At the exit of the driven section holes are drilled (in one plane, 120&#176; apart) to install the three pressure sensors (indicated by *). <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/61244/61244fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/61244/61244fig03.jpg" /> 
Figure 3: Pressure recordings from murine blast wave setup.&#160;(A,B) Peak pressure is scalable and dependent upon polyester membrane thickness. Pressure sensors were used to record peak pressures produced by the shock tube with helium gas and polyester membranes of 25.4, 50.8 or 76.2 &#956;m thickness. (A) At the head placement area, the mean peak pressure produced with 25.4 &#181;m membranes was 428 &#177; 15.9 kPa, with 50.8 &#181;m membranes 637 &#177; 21.4 kPa and with 76.2 &#181;m membranes 1257 &#177; 40.7 kPa (SEM, n = 7-12, one-way ANOVA followed by post-hoc Dunnett&#8217;s comparison test, *** P &#8804; 0.001). (B) At the exit of the shock tube, the mean peak pressure recorded with 25.4 &#181;m membranes was 164 &#177; 11.7 kPa, with 50.8 &#181;m membranes 232 &#177; 11.7 kPa and with 76.2 &#181;m membranes 412 &#177; 11.0 kPa (SEM, n = 7-12, one-way ANOVA followed by post-hoc Dunnett&#8217;s comparison test, ** P &#8804; 0.01, *** P &#8804; 0.001). (C) Representative graph of the pressure recording from the sensor at the head placement area (incident sensor) using a 76.2 &#181;m membrane. The waveform is similar to that of a Friedlander wave, scaled in time/duration for murine subjects. (D) Representative graph of the pressure recording from 3 distinct sensors located at end of the driven section to determine the linearity/phase of waveform within the driven section. All three sensors (located 120 degrees apart) show a similar rise/fall duration indicating that the waveform leaving the driven section is similar in cross-section within the driven section. The blast wave was generated using a 76.2 &#181;m membrane. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/61244/61244fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/61244/61244fig04v4.jpg" /> 
Figure 4: Acute in vivo effects of blast-induced TBI.&#160;(A) Moderate TBI, but not mild TBI increases righting reflex time (RRT). Adult, 3-month-old, male, wild-type C57Bl/6J mice were subjected to TBI procedures using the shock tube with helium gas and polyester membranes of 50.8 or 76.2 &#956;m thickness or sham treatment. Immediately following injury or sham treatment, mice were allowed to recover and RRT was recorded. TBI induction with 50.8 &#956;m membrane or sham treatment exhibited comparable levels of RRT. In contrast, TBI induction using a 76.2 &#956;m membrane increases RRT, indicative of a loss of consciousness induced by the blast wave with the 76.2 &#956;m membrane (SEM, n = 4-10, Sham RRT = 35.6 &#177; 2.0 s, 50.8 &#181;m membrane RRT = 43.0 &#177; 4.3 s and 76.2 &#181;m membrane RRT = 254.0 &#177; 40.2 s, one-way ANOVA followed by post-hoc Dunnett&#8217;s comparison test, *** P &#8804; 0.001). (B) Moderate TBI significantly and transiently reduces core body temperature. Adult, 3-month-old, male, wild-type C57Bl/6J mice were subjected to TBI induction with 76.2 &#181;m membranes or sham treatment. Their core body temperature was recorded for two hours. Baseline core body temperature was recorded prior to TBI induction. Blast-induced TBI with 76.2 &#181;m membranes is associated with a significant drop in core body temperature within the first hour post-TBI. (SEM, n = 10, two-way repeated measures ANOVA, followed by post-hoc Bonferroni&#8217;s multiple comparison tests, ** P &#8804; 0.01, *** P &#8804; 0.001). (C) Moderate TBI results in a transient reduction in body weight. Adult, 3-month-old, male C57Bl/6J mice were subjected to TBI procedures using 76.2 &#181;m membranes or sham treatment. Subsequently, body weights were recorded for 5 days. Total body weight was significantly reduced one-day post-TBI (SEM, n = 7, two-way repeated measures ANOVA followed by post-hoc Bonferroni&#8217;s multiple comparison tests, * P &#8804; 0.05). (D) Moderate TBI results in acute reduction&#160;in locomotor activity. Adult, 3-month-old, male C57Bl/6J mice were subjected to TBI procedures using 76.2 &#181;m membranes or sham treatment. Three hours post-TBI locomotor activity was tracked for 30 minutes and quantified using video tracking software (SEM, n = 9-11, unpaired two-tailed t-test, ** P = 0.01). <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/61244/61244fig04v4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Watch this Scientific Journal Video about Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents at JoVE.com

Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents

We present here a protocol of a blast wave model for rodents to investigate neurobiological and pathophysiological effects of mild to moderate traumatic brain injury. We established a gas-driven, bench-top setup equipped with pressure sensors allowing for reliable and reproducible generation of blast-induced mild to moderate traumatic brain injury.

Traumatic brain injury (TBI) is a large-scale public health problem. Mild TBI is the most prevalent form of neurotrauma and accounts for a large number of ...

low-intensity-blast-wave-model-for-preclinical-assessment-closed-head

Nanyang Technological University

Research

JoVE Journal

Neuroscience

2.6K Views.  Veterans Affairs. We presenteren hier een protocol van een explosiegolfmodel voor knaagdieren om neurobiologische en pathofysiologische effecten van mild tot matig traumatisch hersenletsel te onderzoeken. We hebben een gasgestuurde, bench-top opstelling opgezet die is uitgerust met druksensoren die een betrouwbare en reproduceerbare generatie van door ontploffing geïnduceerd mild tot matig traumatisch hersenletsel mogelijk maken.

Video: Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

Traumatic brain injury (TBI) research has attained renewed momentum due to the increasing awareness of head injuries, which result in morbidity and mortality. Based on the nature of primary injury following TBI, complex and heterogeneous secondary consequences result, which are followed by regenerative processes 1,2. Primary injury can be induced by a direct contusion to the brain from skull fracture or from shearing and stretching of tissue causing displacement of brain due to movement 3,4. The resulting hematomas and lacerations cause a vascular response 3,5, and the morphological and functional damage of the white matter leads to diffuse axonal injury 6-8. Additional secondary changes commonly seen in the brain are edema and increased intracranial pressure 9. Following TBI there are microscopic alterations in biochemical and physiological pathways involving the release of excitotoxic neurotransmitters, immune mediators and oxygen radicals 10-12, which ultimately result in long-term neurological disabilities 13,14. Thus choosing appropriate animal models of TBI that present similar cellular and molecular events in human and rodent TBI is critical for studying the mechanisms underlying injury and repair.
Various experimental models of TBI have been developed to reproduce aspects of TBI observed in humans, among them three specific models are widely adapted for rodents: fluid percussion, cortical impact and weight drop/impact acceleration 1. The fluid percussion device produces an injury through a craniectomy by applying a brief fluid pressure pulse on to the intact dura. The pulse is created by a pendulum striking the piston of a reservoir of fluid. The percussion produces brief displacement and deformation of neural tissue 1,15. Conversely, cortical impact injury delivers mechanical energy to the intact dura via a rigid impactor under pneumatic pressure 16,17. The weight drop/impact model is characterized by the fall of a rod with a specific mass on the closed skull 18. Among the TBI models, LFP is the most established and commonly used model to evaluate mixed focal and diffuse brain injury 19. It is reproducible and is standardized to allow for the manipulation of injury parameters. LFP recapitulates injuries observed in humans, thus rendering it clinically relevant, and allows for exploration of novel therapeutics for clinical translation 20.
We describe the detailed protocol to perform LFP procedure in mice. The injury inflicted is mild to moderate, with brain regions such as cortex, hippocampus and corpus callosum being most vulnerable. Hippocampal and motor learning tasks are explored following LFP.

Lateral fluid percussion (LFP), an established model of traumatic brain injury in mice, is demonstrated. LFP fulfills three major criteria for animal models: validity, reliability and clinical relevance. The procedure, consisting of surgical craniotomy, fixation of hub followed by induction of injury, resulting in focal and diffuse injuries, is described.

Traumatic brain injury (TBI) research has attained renewed momentum due to the increasing awareness of head injuries, which result in morbidity and ...

A Novel Model of Mild Traumatic Brain Injury for Juvenile Rats

Despite growing evidence that childhood represents a major risk period for mild traumatic brain injury (mTBI) from sports-related concussions, motor vehicle accidents, and falls, a reliable animal model of mTBI had previously not been developed for this important aspect of development. The modified weight-drop technique employs a glancing impact to the head of a freely moving rodent transmitting acceleration, deceleration, and rotational forces upon the brain. When applied to juvenile rats, this modified weight-drop technique induced clinically relevant behavioural outcomes that were representative of post-concussion symptomology. The technique is a rapidly applied procedure with an extremely low mortality rate, rendering it ideal for high-throughput studies of therapeutics. In addition, because the procedure involves a mild injury to a closed head, it can easily be used for studies of repetitive brain injury. Owing to the simplistic nature of this technique, and the clinically relevant biomechanics of the injury pathophysiology, the modified weight-drop technique provides researchers with a reliable model of mTBI that can be used in a wide variety of behavioural, molecular, and genetic studies.

The modified weight-drop technique is an easy, cost-effective procedure used for the induction of mild traumatic brain injury in juvenile rats. This novel technique produces clinically relevant symptomology that will advance the study of mild traumatic brain injury (mTBI) and concussion.

Despite growing evidence that childhood represents a major risk period for mild traumatic brain injury (mTBI) from sports-related concussions, motor ...

Medicine

A Novel In Vitro Model of Blast Traumatic Brain Injury

Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the detonation of explosive devices, however, the mechanisms that underlie the brain damage resulting from blast overpressure exposure are not entirely understood and are believed to be unique to this type of brain injury. Preclinical models are crucial tools that contribute to better understand blast-induced brain injury. A novel in vitro blast TBI model was developed using an open-ended shock tube to simulate real-life open-field blast waves modelled by the Friedlander waveform. C57BL/6N mouse organotypic hippocampal slice cultures were exposed to single shock waves and the development of injury was characterized up to 72 h using propidium iodide, a well-established fluorescent marker of cell damage that only penetrates cells with compromised cellular membranes. Propidium iodide fluorescence was significantly higher in the slices exposed to a blast wave when compared with sham slices throughout the duration of the protocol. The brain tissue injury is very reproducible and proportional to the peak overpressure of the shock wave applied.

A Novel In Vitro Model of Blast Traumatic Brain Injury

This paper describes a novel model of primary blast traumatic brain injury. A compressed-air driven shock tube is used to expose in vitro mouse hippocampal slice cultures to a single shock wave. This is a simple and rapid protocol generating a reproducible brain tissue injury with a high throughput.

Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the ...

Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury

The Centers for Disease Control and Injury Prevention estimate that almost 2 million people sustain a traumatic brain injury (TBI) every year in the United States. In fact, TBI is a contributing factor to over a third of all injury-related mortality. Nonetheless, the cellular and molecular mechanisms underlying the pathophysiology of TBI are poorly understood. Thus, preclinical models of TBI capable of replicating the injury mechanisms pertinent to TBI in human patients are a critical research need. The controlled cortical impact (CCI) model of TBI utilizes a mechanical device to directly impact the exposed cortex. While no model can full recapitulate the disparate injury patterns and heterogeneous nature of TBI in human patients, CCI is capable of inducing a wide range of clinically applicable TBI. Furthermore, CCI is easily standardized allowing investigators to compare results across experiments as well as across investigative groups. The following protocol is a detailed description of applying a severe CCI with a commercially available impacting device in a murine model of TBI.

Here we describe a protocol for the induction of murine traumatic brain injury via an open-head controlled cortical impact.

The Centers for Disease Control and Injury Prevention estimate that almost 2 million people sustain a traumatic brain injury (TBI) every year in the ...

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

Highly reproducible animal models of traumatic brain injury (TBI), with well-defined pathologies, are needed for testing therapeutic interventions and understanding the mechanisms of how a TBI alters brain function. The availability of multiple animal models of TBI is necessary to model the different aspects and severities of TBI seen in people. This manuscript describes the use of a midline closed head injury (CHI) to develop a mouse model of mild TBI. The model is considered mild because it does not produce structural brain lesions based on neuroimaging or gross neuronal loss. However, a single impact creates enough pathology that cognitive impairment is measurable at least 1 month after injury. A step-by-step protocol to induce a CHI in mice using a stereotaxically guided electromagnetic impactor is defined in the paper. The benefits of the mild midline CHI model include the reproducibility of the injury-induced changes with low mortality. The model has been temporally characterized up to 1 year after the injury for neuroimaging, neurochemical, neuropathological, and behavioral changes. The model is complementary to open skull models of controlled cortical impact using the same impactor device. Thus, labs can model both mild diffuse TBI and focal moderate-to-severe TBI with the same impactor.

The protocol describes mild traumatic brain injury in a mouse model. In particular, a step-by-step protocol to induce a mild midline closed head injury and the characterization of the animal model is fully explained.

Highly reproducible animal models of traumatic brain injury (TBI), with well-defined pathologies, are needed for testing therapeutic interventions and ...

Assessing Changes in Synaptic Plasticity Using an Awake Closed-Head Injury Model of Mild Traumatic Brain Injury

Mild traumatic brain injuries (mTBIs) are a prevalent health issue in North America. There is increasing pressure to utilize ecologically valid models of closed-head mTBI in the preclinical setting to increase translatability to the clinical population. The awake closed-headed injury (ACHI) model uses a modified controlled cortical impactor to deliver closed-headed injury, inducing clinically relevant behavioral deficits without the need for a craniotomy or the use of an anesthetic.
This technique does not normally induce fatalities, skull fractures, or brain bleeds, and is more consistent with being a mild injury. Indeed, the mild nature of the ACHI procedure makes it ideal for studies investigating repetitive mTBI (r-mTBI). Growing evidence indicates that r-mTBI can result in a cumulative injury that produces behavioral symptoms, neuropathological changes, and neurodegeneration. r-mTBI is common in youths playing sports, and these injuries occur during a period of robust synaptic reorganization and myelination, making the younger population particularly vulnerable to the long-term influences of r-mTBI.
Further, r-mTBI occurs in cases of intimate partner violence, a condition for which there are few objective screening measures. In these experiments, synaptic function was assessed in the hippocampus in juvenile rats that had experienced r-mTBI using the ACHI model. Following the injuries, a tissue slicer was utilized to make hippocampal slices to evaluate bidirectional synaptic plasticity in the hippocampus at either 1 or 7 days following the r-mTBI. Overall, the ACHI model provides researchers with an ecologically valid model to study changes in synaptic plasticity following mTBI and r-mTBI.

Here, it is demonstrated how an awake closed-head injury model can be used for examining the effects of repeated mild traumatic brain injury (r-mTBI) on synaptic plasticity in the hippocampus. The model replicates important features of r-mTBI in patients and is used in conjunction with in vitro electrophysiology.

Mild traumatic brain injuries (mTBIs) are a prevalent health issue in North America. There is increasing pressure to utilize ecologically valid models of ...

Validating Injury Response and Behavioral Assessments in an mTBI Rat Model

Rat Model of Closed-Head Mild Traumatic Injury and its Validation

Animal models are crucial for advancing our understanding of mild traumatic brain injury (mTBI) and guiding clinical research. To achieve meaningful insights, developing a stable and reproducible animal model is essential. In this study, we report a detailed description of a closed-head mTBI model and a representative validation method using Sprague-Dawley rats to verify the modeling effect. The model involves dropping a 550 g mass weight from a height of 100 cm directly onto the head of a rat on a destructible surface, followed by a 180-degree turn. To assess the injury, rats underwent a series of neurobehavioral assessments 10 min post-injury, including time of loss of consciousness, first seeking-behavior time, escape ability, and beam balance ability test. During the acute and subacute stages following the injury, behavioral tests were conducted to assess motor coordination ability (Beam task), anxiety (Open Field test), and learning and memory abilities (Morris Water Maze test). The closed-head mTBI model produced a consistent injury response with minimal mortality and replicated real-life situations. The validation method effectively verified the model development and ensured the stability and consistency of the model.

Author Spotlight: Advancing Traumatic Brain Injury Research - A Closed-Head Model for Accurate Replication and Rapid Assessment 

Here, we present a closed-head mild traumatic brain injury (mTBI) rat model and its validation exhibiting remarkable similarity to human mTBI concerning behavioral manifestations during the acute and subacute stages.

Animal models are crucial for advancing our understanding of mild traumatic brain injury (mTBI) and guiding clinical research. To achieve meaningful ...

rmTBI Induction in Mice via a Closed-Head Injury Method

Modified Mouse Model of Repetitive Mild Traumatic Brain Injury Incorporating Thinned-Skull Window and Fluid Percussion

Mild traumatic brain injury is a clinically highly heterogeneous neurological disorder. Highly reproducible traumatic brain injury (TBI) animal models with well-defined pathologies are urgently needed for studying the mechanisms of neuropathology after mild TBI and testing therapeutics. Replicating the entire sequelae of TBI in animal models has proven to be a challenge. Therefore, the availability of multiple animal models of TBI is necessary to account for the diverse aspects and severities seen in TBI patients. CHI is one of the most common methods for fabricating rodent models of rmTBI. However, this method is susceptible to many factors, including the impact method used, the thickness and shape of the skull bone, animal apnea, and the type of head support and immobilization utilized. The aim of this protocol is to demonstrate a combination of the thinned-skull window and fluid percussion injury (FPI) methods to produce a precise mouse model of CHI-associated rmTBI. The primary objective of this protocol is to minimize factors that could impact the accuracy and consistency of CHI and FPI modeling, including skull bone thickness, shape, and head support. By utilizing a thinned-skull window method, potential inflammation due to craniotomy and FPI is minimized, resulting in an improved mouse model that replicates the clinical features observed in patients with mild TBI. Results from behavior and histological analysis using hematoxylin and eosin (HE) staining suggest that rmTBI can lead to a cumulative injury that produces changes in both behavior and gross morphology of the brain. Overall, the modified CHI-associated rmTBI presents a useful tool for researchers to explore the underlying mechanisms that contribute to focal and diffuse pathophysiological changes in rmTBI.

Author Spotlight: Optimizing Rodent Models for Investigating the Mechanisms and Rehabilitation in Repeated Mild Traumatic Brain Injury

This protocol presents a modified mouse model of repetitive mild traumatic brain injury (rmTBI) induced via a closed-head injury (CHI) method. The approach features a thinned-skull window and fluid percussion to reduce the inflammation commonly caused by meninges exposure, along with improved reproducibility and accuracy in modeling rmTBI in rodents.

Mild traumatic brain injury is a clinically highly heterogeneous neurological disorder. Highly reproducible traumatic brain injury (TBI) animal models ...

Shocktube Method to Model Highly Repetitive Low-Level Blast in Mice

Modeling Highly Repetitive Low-level Blast Exposure in Mice

Exposure to explosive blasts is a significant risk factor for brain trauma among exposed persons. Although the effects of large blasts on the brain are well understood, the effects of smaller blasts such as those that occur during military training are less understood. This small, low-level blast exposure also varies highly according to military occupation and training tempo, with some units experiencing few exposures over the course of several years whereas others experience hundreds within a few weeks. Animal models are an important tool in identifying both the injury mechanisms and long-term clinical health risks following low-level blast exposure. Models capable of recapitulating this wide range of exposures are necessary to inform acute and chronic injury outcomes across these disparate risk profiles.
Although outcomes following a few low-level blast exposures are easily modeled for mechanistic study, chronic exposures that occur over a career may be better modeled by blast injury paradigms with repeated exposures that occur frequently over weeks and months. Shown here are methods for modeling highly repetitive low-level blast exposure in mice. The procedures are based on established and widely used pneumatic shocktube models of open-field blast exposure that can be scaled to adjust the overpressure parameters and the number or interval of the exposures. These methods can then be used to either enable mechanistic investigations or recapitulate the routine blast exposures of clinical groups under study.

Author Spotlight: Deciphering the Long-Term Effects of Low-Level Blast Exposures in Mice

Presented here are methods for producing repeated low-intensity blast exposures using mice.

Exposure to explosive blasts is a significant risk factor for brain trauma among exposed persons. Although the effects of large blasts on the brain are ...

Custom Overpressure Air System for Inducing CNS Injuries in Murine Models

System for Focal, Closed-System Central Nervous System Injury

The prevalence of closed-system central nervous system (CNS) injuries underscores the need for an enhanced understanding of these traumas to improve protective and therapeutic interventions. Crucial to this research are animal models that replicate closed-system CNS injuries. In this context, a custom overpressure air system was engineered to reproduce a range of closed-system CNS injuries in murine models, including ocular, brain, and spinal cord trauma. To date, the system has been used to administer eye-, head-, or spine-directed overpressure air to model anteroposterior pole injury in the eye, indirect traumatic optic neuropathy (ITON), focal traumatic brain injury, and spinal cord injury. This paper provides a detailed protocol outlining the system&#39;s design and operation and shares representative results demonstrating its effectiveness. The robust framework presented here provides a strong foundation for ongoing research in CNS trauma. By leveraging the system&#8217;s flexible attributes, investigators can modify and carefully control the location, severity, and timing of injuries. This allows for comprehensive comparisons of molecular mechanisms and therapeutic efficacy across multiple closed-system CNS injuries.

Author Spotlight: Developing Precise and Clinically Relevant Models for Studying Secondary Degeneration in Traumatic Optic Neuropathy

This protocol describes a custom overpressure air system designed to induce closed-system central nervous system (CNS) injuries in mice, including ocular, brain, and spinal cord traumas. The goal of this protocol is to provide a framework for researchers to easily adapt and expand the system for their unique CNS trauma studies.

The prevalence of closed-system central nervous system (CNS) injuries underscores the need for an enhanced understanding of these traumas to improve ...

Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents

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