We would like to thank Kevin Quant, Mario Martinez, and Edwin Portillo for scoring nests, Rachel Barkey for assisting in the preparation for filming, and Stephen Lippi and Deborah Neely for proofreading this manuscript. We would also like to thank the Department of Psychology for covering the costs of cages for this experiment, and the Krasnow Institute Animal Facility caretakers for their support.

All procedures were approved by the George Mason University Institutional Animal Care and Use Committee and are in accordance with guidelines set forth by the Assessment and Accreditation of Laboratory Animal Care.
1. Animals and considerations prior to the assessment
<ol>
	<li>For this protocol, use adult, 9 - 12-month-old C57BL/6J (n = 10) (stock # 000664) wildtype mice and APOE e4 mice (n = 11) from a hemizygous J20 (stock #006293) x homozygous APOE e4 (stock #012307) cross.</li>
	<li>In the housing room, group-house mice with littermates of the same sex with appropriate enrichment (e.g., for this protocol, mice were provided a running wheel, igloo, and a small nylon chew toy). Group-house 4 - 6 female mice, and 4 males in a 356 mm L x 485 mm W x 218 mm H home cage. 
	NOTE: Researchers may consider implementing strategies prior to and/or after nesting in order to avoid cage-mate aggression when mice are reintroduced after nesting trials. Such strategies may include, but are not limited to, daily handling prior to behavioral testing to better acclimate mice to handling during behavioral testing, researchers, and husbandry staff19, separating and individually housing aggressive mice, or reducing the number of mice in the homecage20, depending on the severity of in-cage fighting, observed wounds21, etc.</li>
</ol>
2. Room and nesting set-up
<ol>
	<li>Ensure that each mouse completes four trials (1 material per trial). Randomize the order of nesting material for each mouse to avoid an order effect.</li>
	<li>Prepare the cages in a separate testing room. Record environmental conditions (e.g., 22.2 &#8211; 22.3&#176;C, 45-47% humidity, lights on 9:00 AM &#8211; 9:00 PM) such that they are consistent across trials and are identical to the housing room. Provide food and water ad libitum.
	<ol>
		<li>Assign each mouse a random identification (ID) number or letter. Attach the random identifier card to a 29.2 x 18.4 cm cage.</li>
		<li>Record the original animal ID/tag and other necessary identification in a colony record to ensure that assistants and animal husbandry staff remain blind to conditions.</li>
	</ol>
	</li>
	<li>Randomly order cage placement in the testing room such that wildtype and transgenic mice are not inappropriately separated (i.e., on opposite sides of the room, on separate shelves, etc.).</li>
	<li>Prepare the nesting materials by sufficiently covering the bottom of the cage. Use approximately 100 g dry weight of corncob bedding for the square, twist, and shredded paper trials, and approximately 100 g of the soft cob bedding for the soft cob trial.</li>
	<li>If using a beaker to disperse the nesting material, then fill the beaker no more than 100 mL with corncob bedding or soft cob bedding.</li>
	<li>Place the first nesting material in the sequence prior to introducing the mouse into the cage. This protocol utilized (1) a single pressed cotton square, (2) a single paper twist, (3) 100g of soft cob bedding only (i.e., no additional bedding or nesting material added), and (4) 2.5 g of clean (no ink), shredded white printer paper cut into 5 &#8211; 7cm strips. Disperse nesting materials as shown in Figure 1 (baseline).</li>
</ol>
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/60139/60139fig1v3.jpg" /> 
Figure 1: Cage set-up for each material. All mice completed one trial with each type of material for a total of four trials. Corncob bedding lined the entire bottom of the cages containing a paper twist, a pressed cotton square, and shredded paper. Soft cob bedding was evenly dispersed across the cage to encourage mice to separate out the small cotton squares from the corncob. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/60139/60139fig1v3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
3. Nesting trial
<ol>
	<li>Begin the first nesting trial at the same time of day at the start of the light cycle (e.g., 9:00 AM).</li>
	<li>Bring the homecage containing the mice into the testing room. Remove each mouse from the homecage and place it into its assigned nesting cage with the material already placed in the cage. Return the homecages to the housing room.</li>
	<li>Allow mice to complete 1 trial for 24 h undisturbed.</li>
	<li>24 h after the start of the first trial, return to the testing room.</li>
	<li>Carefully remove the lid of the cage and photograph each mouse&#8217;s nest, capturing the assigned ID in the photograph and minimizing the appearance of any materials outside of the cage. 
	NOTE: Wait for the mouse to move off the nest before attempting to remove it from the cage. It is strongly recommended to photograph the nest while the mouse is in the cage but off the nest. Attempting to remove the mouse from the cage before photographing can potentially cause the mouse to become startled, thus move on top of, and disperse, nesting materials.</li>
	<li>Gently remove the mouse from the nesting cage and place it in a temporary holding cage. Dispose of the bedding and any nesting materials, replace the bedding and provide the next nesting material in the sequence, and return the mouse to the nesting cage.</li>
	<li>Repeat as necessary to obtain scores for all 4 trials. It is recommended to use shredded paper and the accompanying scoring criteria, although researchers may be interested in using the alternative materials discussed in this protocol.</li>
	<li>When all trials are complete, return mice to their home cages. Observe the mice for any potential aggressive behavior. Aggression may occur in older wildtype males. 
	NOTE: For the purposes of this protocol, mice were tested once at approximately 9 &#8211; 12 months of age. Nesting should be conducted at several ages (i.e., at an earlier age, dependent on the chosen strain, prior to the onset of phenotypic traits) to document the decrease in nesting capacity over time and demonstrate the likely causative role of neurodegeneration.</li>
</ol>
4. Scoring and assessing inter-rater reliability
<ol>
	<li>Provide baseline images for each nesting material to at least 2 individuals blind to the study. Although not required, ensure that the scorers are familiar with the concept of nesting. When training scorers, provide a series of example nests (e.g., Figure 2 for shredded paper) to familiarize the scorers with each type of material (if applicable) and the scoring criteria.
	<ol>
		<li>Evaluate each nest on a scale of 1 &#8211; 5 using the following scale information (adapted from Deacon, 2006)2. See Figure 2 as example scoring using shredded paper.</li>
		<li>Assign a score of 1 when the shredded paper or small squares remained scattered throughout the cage, or the cotton square or twist remained untouched;</li>
		<li>Assign a score of 2 when some of the material was constructed into a nest, but over 50% of the material was not used for nest construction (i.e., remained scattered or the majority of the original material remained untouched);</li>
		<li>Assign a score of 3 when a noticeable nest was constructed, but several pieces were still scattered;</li>
		<li>Assign a score of 4 when almost all the material was used for the nest, but few pieces of material remained scattered or were near the nest;</li>
		<li>Assign a score of 5 when all material was used to make an identifiable nest. 
		NOTE: Scorers should take breaks and revisit baseline photos in order to avoid fatigue and bias during the scoring procedure. Scorers should not discuss scores with one another to avoid bias. Scorers should discuss scores after scoring is complete, or if further discussion is needed to change scores, which can potentially resolve issues with intraclass correlations (ICC). Intraclass correlations will be conducted using the steps listed in section 4.2.</li>
	</ol>
	</li>
</ol>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/60139/60139fig2.jpg" /> 
Figure 2: Example of scoring using the criteria for shredded paper, the preferred material. From left to right: 1 &#8211; the shredded paper remained untouched; 2 &#8211; some of the paper was constructed into a nest but most of the pieces remained scattered; 3 &#8211; a noticeable nest was constructed, but several pieces were still scattered; 4 &#8211; most pieces were used for the nest, but some pieces were scattered near the nest; 5 &#8211; all pieces were used to make the nest. Note that in the photographs, the animal&#8217;s assigned number is shown to avoid unblinding. The color of the card is random. Researchers should allow the mouse to remain in the cage in order to avoid startling the mouse, which could potentially disperse the nest. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/60139/60139fig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<ol start="2">
	<li>Collect and input scores and format the data file such that each scorer&#8217;s scores for each material are in separate columns. 
	NOTE: If using Supplementary File 1, please note that the coding script for this analysis requires free, open source softwares (e.g., irr package in RStudio)22,23. The script removes headers and subjects variables (i.e., the top row and animal IDs) to conduct the intraclass correlation (ICC). Run the script to conduct a two-way, agreement, averages measures ICC by highlighting the demarcated section of the code and hitting Ctrl+Enter or Command+Enter.</li>
	<li>Compare the ICC to existing criteria24,25,26. Typically, ICC values above 0.80 correspond to strong inter-rater reliability, justifying scores to be averaged at a given time point (Supplementary File 2).</li>
	<li>Use the same ICC criteria for intra-rater reliability, which may be of interest for those conducting scoring for the first time. 
	CAUTION: Scorers should score the same photographed nests within a week of the initial assessment. Proceed with caution with averaging data when ICC is low, as this may skew data or produce null findings. If ICC values are low, then (1) additional training for scorers may be required, (2) scorers may need to discuss their reasoning for nest scores, (3) another independent reviewer may be needed to make a judgment call regarding the scores, or (4) intra-rater reliability may need to be assessed. If intra-rater reliability is poor, then additional training or a different scorer may be required.</li>
</ol>
5. Statistical considerations
<ol>
	<li>Conduct statistical analyses, as appropriate. For data that do not violate normality assumptions but use multiple materials for each mouse (i.e., a within-subjects effect), use a mixed analysis of variance (ANOVA).
	<ol>
		<li>If using codes provided as supplemental files, please download additional add-on packages which are specified in the script27,28,29,30.</li>
		<li>If conducting a mixed ANOVA using the provided script, run the script to convert any wide-format data files into long-format (i.e., instead of variables repeated across Columns, convert the cells to repeat across Rows). An example data file is included in this manuscript to demonstrate how to convert a data file from wide-format to long-format.</li>
		<li>Conduct the mixed ANOVA, as specified in the script. Note that if Mauchly&#8217;s test of Sphericity is violated, then implement correction factors such as Greenhouse-Geisser correction.</li>
		<li>Conduct any necessary posthoc tests to examine differences among factors with more than 2 levels, as specified in the coding script. In this example, conduct Bonferroni posthoc tests to compare the different types of nesting material. 
		NOTE: If only using one nesting material, then do not incorporate a within-subjects factor. If collecting data at multiple time points, however, incorporate these repeated-measures as if levels of a within-subjects factor. 
		&#8203;NOTE: In some cases, wildtype mice may have perfect scores and thus will exhibit a ceiling effect, leading to a non-normal distribution2. Consider appropriate statistical tests (e.g., nonparametric tests with alternative measures of central tendency and dispersion), normalization methods, or robust approaches (mixed effects modeling for repeated measures) when analyzing data.</li>
	</ol>
	</li>
</ol>

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The authors have nothing to disclose.

Nesting is an evolutionarily important rodent behavior and has been used to assess the activity of daily living and general well-being in mice2. The ease to conduct the test, its reliability, and its face validity make&#160;nesting a practical complement to many behavioral tests such as burrowing, circadian rhythm, and grooming. But, as nesting becomes more commonly utilized in the laboratory, the various combinations to conduct, quantify, and interpret nesting increase. As such, further research is needed to explore the best methodologically and practically sound procedures for nesting, such that the validity and reliability of the assay are not sacrificed for costs, time of testing, and other procedures that reduce testing burden.
Nest building quality is indeed sensitive to the type of bedding provided as well as genotype. Overall, wildtype mice built significantly better nests compared to APOE e4 mice regardless of the nesting material; however, both wildtype and APOE e4 mice build significantly higher quality nests out of shredded paper compared to the three other nesting materials. Other studies provide corroborating evidence regarding the shredded paper: mice constructed more complex nests out of shredded paper strips than with pressed cotton squares, tissue, and aspen bedding33. Furthermore, nests constructed out of shredded paper strips have been qualitatively evaluated as more &#34;naturalistic&#34;&#160;than those made of other materials, which are characterized by the shape of the nest itself and the height of the walls around the nest cavity in order to form a dome6,33. As such, selection of proper material for this assay is critical in order to better observe natural behavior in a relatively controlled environment, i.e., the laboratory setting. More importantly, although this protocol assessed nesting once at 9 &#8211; 12 months of age, we emphasize that nesting should be conducted at several ages. The simplicity of this protocol permits it to be conducted several times, ideally before the onset of deficits that accompany neurodegeneration. Repeated measurements afford the opportunity to document the likely causative role of neurodegeneration in decreased nesting ability.&#160;
Natural nest building has been shown to differ among mice of different background strains34, and as such, the overall quality and shape of the nest may differ not due to the transgene of interest, but due to the background strain. For example, the ancestors of C57BL/6 mice were considered &#34;hole&#34;&#160;nesters, whereas the ancestors of BALB/C mice were considered &#34;surface&#34;&#160;nesters35. Mice on the C3H background or crosses with this strain, such as the hybrid C3H/He-C57BL/6 with E4 used in Graybeal et al.9 are also considered to be poor nesters; thus, researchers should strongly consider using control mice on the same background as transgenic mice, which would overall improve the direct, causative role of the transgene(s), rather than the background, in neurodegeneration and subsequent deficits in nest building behavior.
Some experiments often utilize a single scorer to qualitatively judge the complexity of the nest; however, we make the argument to include more scorers, and more importantly, scorers blind to experimental conditions. Through this approach, we utilized three independent, blind scorers and an intraclass correlation to assess the agreement among the scorers, who, with basic training, yielded high intraclass correlations which were indicative of high inter-rater reliability and strong agreement regarding nest quality. Furthermore, scores of nests composed of shredded paper and cotton squares had the highest intraclass correlations, an indication of stronger agreement and greater consistency among the scorers. The strong agreement also provides justification to average the multiple scores together, a strategy haphazardly implemented in behavioral research. Although this strategy requires more individuals and, presumably, more time to score nests, it effectively reduces bias in qualitative assessments such as nesting.
The nesting materials used in this assay were approximately equal in price except for the soft cob bedding. Commercial bedding for nesting may be economically resourceful for some experimenters; however, Martin et al.15 note that cotton squares, when compared to other materials such as crinkled paper, is one of the most expensive materials available for purchase. This may be due to easy availability, storage, and administration, but other researchers may opt for similarly valid and reliable materials, especially when challenged by large numbers of animals in a facility, the number of nesting trials, time restrictions for scoring nests, and high cage costs. Thus, shredded paper may be a more feasible and appropriate option. In addition, data capture for our method can be conducted immediately (i.e., scoring in person), although photographs should be highly considered in order to record, save, and re-quantify nests, if desired for the purposes of assessing inter- and intra-rater reliability at later times. As noted, we strongly emphasize the inclusion of multiple scorers for &#34;test, re-test&#34;&#160;practices to assess for agreement, as these methodologically sound procedures are often overlooked.
In conclusion, we believe that the methodology and statistical considerations (i.e., intraclass correlation) for this assay may be of interest for experiments assessing the quality of living, general well-being, and activities of daily living in mice.

Nest building is an innate behavior in many animals such as birds, fish, rodents, and great apes, and it is attracting more attention for its potential utility in the study of neurological diseases and disorders characterized by diminished well-being and impaired activities of daily living1. Mice, both male and female, build similarly-sized nests for reproductive purposes, heat conservation, and shelter; more importantly, they continue to do so even when raised in laboratory settings2. Hess, Petrovich, and Goodwin3 argue that biologically-appropriate stimuli are paramount to induce biologically-appropriate behaviors such as nesting; however, a variety of natural and artificial materials, such as hay, hemp, cotton twine, paper strips, cotton dental balls, and pressed cotton squares, have been used to assess nest building behavior2. Changes in these nesting behaviors (i.e., failure to create a nest out of the provided material) generally indicate a change in health or welfare. In most cases, a lack of nest building is attributed to several factors that negatively impact well-being. Such examples that have all effectively decreased the quality of nest building in mice include temperature extremes; painful stimulation; induced illness and infection; genetic mutations; and brain lesions in the medial preoptic areas, septum, and hippocampus4,5,6.
Alzheimer&#8217;s disease is a progressive neurodegenerative disease characterized by loss of brain tissue, accumulation of extracellular amyloid-&#946; plaques, and intracellular neurofibrillary tangles composed hyperphosphorylated microtubule-associated protein, tau7. Additionally, Alzheimer&#8217;s disease is characterized by, most notably, deficits in learning and memory and impairments of activities of daily living. In mice, activities of daily living are commonly examined via circadian wheel-running8,9,10,11, although new alternatives, such as nesting, are growing in popularity. Nesting has been shown to be sensitive to manipulations (e.g., genetic mutations, environmental stressors) that have been identified as risk factors and/or causes of Alzheimer&#8217;s disease. As such, nesting can be used as an additional or complementary assay in many mouse strains that model those characteristics of Alzheimer&#8217;s disease. For example, Deacon and colleagues12 measured nest building of Tg2576 mice with the Swedish amyloid precursor protein (APPswe) and presenilin 1 (PS1) genetic mutations. The quality of nests constructed by group-housed Tg2576 mice was significantly worse than wildtype controls at both 3- and 12-months of age. In agreement with these findings, Filali et al.13 reported that singly housed APPswe/PS1 male mice given two pieces of 5 x 5 cm cotton built significantly poorer nests as rated on a scale of 1 - 5 (1 = nestlet not touched, 5 = a near perfect nest). Transgenic mice consistently built worse nests at 6-, 9-, and 12-months of age compared to their wildtype counterparts, and in some cases, the Appswe/PS1 mice completely failed to build a nest out of the provided cotton.
Previous research from our lab has demonstrated that wildtype C57BL/6J mice build significantly better nests out of cotton squares compared to CRND8 and CRND8/E4 mice9. However, the majority of experiments using pressed cotton squares appear to be variable, with wildtype mice failing to show expected high scores compared to transgenic mice that are expected to show very low scores in nesting2, which may in part lead to a lack of differences in estimated parameters (i.e., mean differences) and statistical significance. The lack of differences may be due in part to inadequate aging of mice or time allotted for nesting. Alternatively, nesting material may be an additional challenge, resulting in more variability due to researchers&#8217; methodological preferences in the quantity and type of material, which may even interact with mouse strain. For example, Robinson-Junker and colleagues14 provided processed or unprocessed bedding material of different sizes (i.e., small or large flakes) to C3H/HeNCrl mice and BALB/cAnNCrl mice, which are commonly observed, respectively, as poor and strong nest builders. When provided unprocessed bedding, C3H mice built less complex, yet similar in quality nests compared to those of BALB/c mice.&#160; Likewise, Martin and colleagues15 compared nest complexity of different nesting materials given to deer mice, a distant relative of the Mus musculus species that have distinct evolutionary differences (i.e., more likely to burrow in trees and underbrush and are more active in captivity), but receive similar husbandry care as common laboratory mice and build nests out of any available soft, fibrous material16,17,18.&#160; Females and breeders with pups in the home cage built more complex nests than males, and the authors suggest that these behavioral differences may be due to associated changes in progesterone concentrations in deer mice15. More importantly, mice built more complex nests composed of brown paper followed by cotton squares and cotton cylinders, and the least complex nests were constructed out of white paper and dispersed mini-cotton squares.
Despite the growing popularity of nesting, considerations regarding scientifically valid, cost-effective, and time-sensitive practices are minimally discussed. Given the aforementioned methodological and economical challenges, this protocol investigates the utility of different nesting materials &#8211; cotton squares, paper twists, shredded paper, and processed bedding &#8211; in nesting behavior. Specifically, we provided all nesting materials to both aged C57BL/6J wildtype controls and Alzheimer&#8217;s disease APOE e4 mice in order to investigate any potential genotype by material interactions in nesting quality. Additionally, the experiment sought to assess inter-rater reliability of nests constructed out of different materials. Taken together, this protocol demonstrates the superiority of one nesting material in this sample &#8211; shredded paper &#8211; in terms of nest quality and scorer agreement, with the intention of improving the validity and reliability of the nesting assay.

<table><tbody><tr><td>Corncob bedding</td><td>Envigo</td><td>7092</td><td>1/8 in bedding for cotton squares, shredded paper, and paper twist trials</td></tr><tr><td>Cotton Squares</td><td>Envigo</td><td>Iso-Blox</td><td></td></tr><tr><td>Diamond Twists</td><td>Envigo</td><td>7979C.CS</td><td>Paper twists used in protocol</td></tr><tr><td>Mouse - APOE4 e4</td><td>Jackson Laboratories</td><td>#012307</td><td>Homozygous APEO4 e4 mouse bred with hemizyous J20 mouse</td></tr><tr><td>Mouse - C57BL/6J</td><td>Jackson Laboratories</td><td>#000664</td><td>Wildtype mouse for controls</td></tr><tr><td>Mouse - J20</td><td>Jackson Laboratories</td><td>#006293</td><td>Hemizygous mouse bred with the homozygous APOE e4 mouse to generate cross</td></tr><tr><td>Rstudio</td><td>R Core Team</td><td>V1.1.463</td><td>Run with R version 3.5.3 (2019-03-11) -- &quot;Great Truth&quot;. Note: additional R Packages are included in provided code and can be installed from CRAN</td></tr><tr><td>Soft Cob</td><td>Envigo</td><td>7087C</td><td></td></tr></tbody></table>

nest building behavior as an early indicator of behavioral deficits in mice

Nest building is an innate behavior in male and female rodents, even when raised in laboratory settings. As such, many researchers provide rodents synthetic and/or natural materials (such as twine, tissue, cotton, paper, and hay) as a gauge of their overall well-being and as an ancillary assessment to predict the possible decline in cognition. Typically, changes in nesting behaviors, such as failure to create a nest, indicate a change in health or welfare. In addition, nesting behavior is sensitive to many environmental and physiological challenges, as well as many genetic mutations underlying pathological disease states. The following protocol describes a nesting behavior paradigm that explores the usage of four types of nesting material. In addition, the protocol utilizes intraclass correlations to demonstrate that inter-rater reliability is higher when nests are constructed out of shredded paper compared to other common nesting materials such as cotton squares, paper twists, and soft cob bedding. The chosen methodology and statistical considerations (i.e., intraclass correlation) for this assay may be of interest for those conducting experiments assessing the quality of living of mice.&#160;

Results from the four different nesting materials provided to wildtype and APOE e4 mice are explained as follows. Based on existing criteria, the ICC showed a strong agreement among all three coders for all four nesting materials (shredded paper ICC = 0.94; square ICC = 0.91; bedding ICC = 0.87; twist ICC = 0.87); therefore, the three scores were averaged together to create a single score for each material provided. A 2 x 4 mixed ANOVA yielded a significant main effect of genotype, F(1, 19) = 31.30, p &#60; &#160;0.01, &#951;p2 = 0.62. Across all four provided materials, wildtype mice scored higher on nest quality (3.18 &#177; 0.20) compared to APOE e4 mice (1.98 &#177; 0.16). In addition, the mixed ANOVA yielded a significant main effect of material, F(3, 57) = 57.48, p &#60; 0.01, &#951;p2 = 0.75. Pairwise comparisons with Bonferroni correction showed that the shredded paper (4.11 &#177; 0.20) was rated significantly higher (p &#60; 0.05) in quality than the square (1.95 &#177; 0.21), bedding (2.21 &#177; 0.21), or twist (1.94 &#177; 0.20) materials, with no differences seen between the square, bedding, and twist materials (all p &#62; 0.99). There was no significant interaction between genotype and material. Data are shown in Figure 3.
Other experiments from our laboratory have yielded similar results in early-onset models of Alzheimer&#8217;s disease. For example, as demonstrated in Figure 4, 5.5-month-old P301L rTg4510 (htau) mice built significantly worse nests out of shredded paper compared to their age-matched wildtype counterparts31. Likewise, dual J20 (hAPP)/htau and single htau mice built poorer nests out of shredded paper compared to their wildtype and CAMKIIa-promoter only counterparts, both at 3.5- and 7-months of age32 (data not shown). &#160;
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/60139/60139fig3v2.jpg" /> 
Figure 3: Rated quality of nests made from different materials provided to wildtype and APOE e4 mice. A main effect of genotype (*p &#60; 0.01) demonstrated that wildtype mice consistently built better nests made from the shredded paper, pressed cotton squares, paper twist, and soft cob bedding compared to APOE e4 mice. The main effect of material (**p &#60; 0.01) also demonstrated that nests constructed from the shredded paper were rated higher in quality compared to the three other materials. The shredded paper had the highest inter-rater reliability measure as assessed by ICC. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/60139/60139fig3v2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/60139/60139fig4.jpg" /> 
Figure 4: Shredded paper nests built by wildtype and htau mice, on or off zinc water. (A) Representative nests constructed by 5.5-month-old wildtype and htau mice, on control (tap) water or water supplemented with 10 parts per million zinc (a biometal implicated in Alzheimer&#8217;s disease). Nests were scored on the 1 - 5 scale using the specified criteria. From left to right: wildtype + zinc water (4.54&#177;0.52); wildtype + control water (4.15&#177;0.80); htau + control water (3.08 &#177; 0.64); htau + zinc water (2.46&#177;0.97). (B) A genotype*water interaction demonstrated that htau mice on zinc water constructed significantly worse nests compared to the other groups (*); in addition, simple effects showed that htau mice on zinc water built worse nests than htau mice on control water. Wildtype mice on control water and zinc water-built nests of higher, and similar quality, compared to their htau counterparts. Reprinted from Journal of Alzheimer&#8217;s Disease, Vol 64, Craven, KM, Kochen WR, Hernandez CM, Flinn JM, Zinc Exacerbates Tau Pathology in a Tau Mouse Model, 671-630, Copyright (2018), with permission from IOS Press31. The publication is available at IOS Press through https://dx-doi-org.remotexs.ntu.edu.sg/10.3233/JAD-180151. <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/60139/60139fig4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Supplementary File 1" src="/files/ftp_upload/60139/60139supfig1.jpg" /> 
Supplementary File 1: Supplemental Coding File &#8211; nesting.R <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/60139/suppfile1.R" target="_blank">Please click here to download this file.</a>
<img alt="Supplementary File 2" src="/files/ftp_upload/60139/60139supfig2.jpg" /> 
Supplementary File 2: AD Study nesting scores.csv <a href="https://www-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/60139/suppfile2.csv" target="_blank">Please click here to download this file.</a>

Watch this Scientific Journal Video about Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice at JoVE.com

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Here, we present a protocol to quantify nest building behavior in mice, which is known to be impaired in several neurological disorders and diseases. This protocol examines the utility of four materials and offers the opportunity to quantify the rater agreement in scoring, improving the validity and reliability of the assay.

Nest building is an innate behavior in male and female rodents, even when raised in laboratory settings. As such, many researchers provide rodents ...

nest-building-behavior-as-an-early-indicator-behavioral-deficits

Nanyang Technological University

Research

JoVE Journal

Behavior

19.7K Views.  George Mason University. Nestgedrag is een nuttige manier om activiteiten van het dagelijks leven in wilde en transgene muizen te beoordelen. Het is vooral handig bij het documenteren van cognitieve achteruitgang in neurodegeneratie over de levensduur. Deze techniek is een economische eenvoudige en elegante beoordeling van de dagelijkse levende activiteiten in muizen.Het kan ook worden uitgevoerd in een korte periode van tijd met minimale training. In ons laboratorium raden we versnipperd papier aan, omdat pap...

Video: Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Marble Burying and Nestlet Shredding as Tests of Repetitive, Compulsive-like Behaviors in Mice

Obsessive-compulsive disorder (OCD) and autism spectrum disorders (ASD) are serious and debilitating psychiatric conditions and each constitutes a significant public health concern, particularly in children. Both of these conditions are highlighted by the repeated expression of meaningless behaviors. Individuals with OCD often show checking, frequent hand washing, and counting. Children with ASDs also engage in repetitive tapping, arm or hand flapping, and rocking. These behaviors can vary widely in intensity and frequency of expression. More intense forms of repetitive behaviors can even result in injury (e.g.&#160;excessive grooming, hand washing, and self-stimulation). These behaviors are therefore very disruptive and make normal social discourse difficult. Treatment options for repetitive behaviors in OCD and ASDs are somewhat limited and there is great interest in developing more effective therapies for each condition. Numerous animal models for evaluating compulsive-like behaviors have been developed over the past three decades. Perhaps the animal models with the greatest validity and ease of use are the marble burying test and the nestlet shredding test. Both tests take advantage of the fact that the target behaviors occur spontaneously in mice. In the marble burying test, 20 marbles are arrayed on the surface of clean bedding. The number of marbles buried in a 30 min session is scored by investigators blind to the treatment or status of the subjects. In the nestlet shredding test, a nestlet comprised of pulped cotton fiber is preweighed and placed on top of cage bedding and the amount of the nestlet remaining intact after a 30 min test session is determined. Presently, we describe protocols for and show movie documentation of marble burying and nestlet shredding. Both tests are easily and accurately scored and each is sensitive to small changes in the expression of compulsive-like behaviors that result from genetic manipulations, disease, or head injury.

Disease, head injury, genetic modifications, and treatment of mice with drugs can have profound effects on behavior. Utilizing well-characterized and validated approaches such as marble burying and nestlet shredding, compulsive-like behaviors can be documented accurately in mice as models of human obsessive-compulsive disorder and autism spectrum disorder.

Obsessive-compulsive disorder (OCD) and autism spectrum disorders (ASD) are serious and debilitating psychiatric conditions and each constitutes a ...

Nest Building as an Indicator of Health and Welfare in Laboratory Mice

The minimization and alleviation of suffering has moral and scientific implications. In order to mitigate this negative experience one must be able to identify when an animal is actually in distress. Pain, illness, or distress cannot be managed if unrecognized. Evaluation of pain or illness typically involves the measurement of physiologic and behavioral indicators which are either invasive or not suitable for large scale assessment. The observation of nesting behavior shows promise as the basis of a species appropriate cage-side assessment tool for recognizing distress in mice. Here we demonstrate the utility of nest building behavior in laboratory mice as an ethologically relevant indicator of welfare. The methods presented can be successfully used to identify thermal stressors, aggressive cages, sickness, and pain. Observation of nest building behavior in mouse colonies provides a refinement to health and well-being assessment on a day to day basis.

We demonstrate the utility of nest building behavior in laboratory mice as an indicator of welfare. Nest scoring is a sensitive technique that is altered by temperature, illness, and aggression. The time to integrate into nest test (TINT) is a simple cage-side assessment that can detect postoperative pain.

The minimization and alleviation of  suffering has moral and scientific implications. In order to mitigate this  negative experience one must be able to ...

Behavioral Phenotyping of Murine Disease Models with the Integrated Behavioral Station (INBEST)

Due to rapid advances in genetic engineering, small rodents have become the preferred subjects in many disciplines of biomedical research. In studies of chronic CNS disorders, there is an increasing demand for murine models with high validity at the behavioral level. However, multiple pathogenic mechanisms and complex functional deficits often impose challenges to reliably measure and interpret behavior of chronically sick mice. Therefore, the assessment of peripheral pathology and a behavioral profile at several time points using a battery of tests are required. Video-tracking, behavioral spectroscopy, and remote acquisition of physiological measures are emerging technologies that allow for comprehensive, accurate, and unbiased behavioral analysis in a home-base-like setting. This report describes a refined phenotyping protocol, which includes a custom-made monitoring apparatus (Integrated Behavioral Station, INBEST) that focuses on prolonged measurements of basic functional outputs, such as spontaneous activity, food/water intake and motivated behavior in a relatively stress-free environment. Technical and conceptual improvements in INBEST design may further promote reproducibility and standardization of behavioral studies.

Behavioral Phenotyping of Murine Disease Models with the Integrated Behavioral Station INBEST

Prolonged and comprehensive monitoring of mice in a home-cage environment provides a deeper understanding of aberrant behavior in murine models of brain diseases. This paper describes the Integrated Behavioral Station (INBEST) as the key component of contemporary behavioral analysis.

Due to rapid advances in genetic engineering, small rodents have become the preferred subjects in many disciplines of biomedical research. In studies of ...

Assessing Burrowing, Nest Construction, and Hoarding in Mice

Deterioration in the ability to perform "Activities of daily living" (ADL) is an early sign of Alzheimer's disease (AD). Preclinical behavioural screening of possible treatments for AD currently largely focuses on cognitive testing, which frequently demands expensive equipment and lots of experimenter time. However, human episodic memory (the most severely affected aspect of memory in AD) is different to rodent memory, which seems to be largely non-episodic. Therefore the present ways of screening for new AD treatments for AD in rodents are intrinsically unlikely to succeed. A new approach to preclinical screening would be to characterise the ADL of mice. Fortuitously, several such assays have recently been developed at Oxford, and here the three most sensitive and well-characterised are presented.
Burrowing was first developed in Oxford13. It evolved from a need to develop a mouse hoarding paradigm. Most published rodent hoarding paradigms required a distant food source to be linked to the home cage by a connecting passage. This would involve modifying the home cage as well as making a mouse-proof connecting passage and food source. So it was considered whether it would be possible to put the food source inside the cage. It was found that if a container was placed on the floor it was emptied by the next morning., The food pellets were, however, simply deposited in a heap at the container entrance, rather than placed in a discrete place away from the container, as might be expected if the mice were truly hoarding them. Close inspection showed that the mice were performing digging ("burrowing") movements, not carrying the pellets in their mouths to a selected place as they would if truly hoarding them.6



Food pellets are not an essential substrate for burrowing; mice will empty tubes filled with sand, gravel, even soiled bedding from their own cage. Moreover, they will empty a full tube even if an empty one is placed next to it8.



Several nesting protocols exist in the literature. The present Oxford one simplifies the procedure and has a well-defined scoring system for nest quality5. 



A hoarding paradigm was later developed in which the mice, rather than hoarding back to the real home cage, were adapted to living in the "home base" of a hoarding apparatus. This home base was connected to a tube made of wire mesh, the distal end of which contained the food source. This arrangement proved to yield good hoarding behaviour, as long as the mice were adapted to living in the "home base" during the day and only allowed to enter the hoarding tube at night.

Burrowing, nesting, and hoarding are species-typical activities that mice readily perform in the laboratory. This article describes how they can be easily and cheaply assessed. These protocols are extremely sensitive to mouse strain, brain lesions and diseases. Moreover they constitute &#x201C;environmental enrichment&#x201D; for the mice, and embody the &#x201C;Refinement&#x201D; aspect of the &#x201C;3 Rs&#x201D;18.

Deterioration in the ability to perform "Activities of daily living" (ADL) is an early sign of Alzheimer's disease (AD). Preclinical behavioural screening ...

Neuroscience

Novel Object Exploration as a Potential Assay for Higher Order Repetitive Behaviors in Mice

Restricted, repetitive behaviors (RRBs) are a core feature of autism spectrum disorder (ASD) and disrupt the lives of affected individuals. RRBs are commonly split into lower-order and higher-order components, with lower order RRBs consisting of motor stereotypies and higher order RRBs consisting of perseverative and sequencing behaviors. Higher order RRBs are challenging to model in mice. Current assays for RRBs in mice focus primarily on the lower order components, making basic biomedical research into potential treatments or interventions for higher-order RRBs difficult. Here we describe a new assay, novel object exploration. This assay uses a basic open-field arena with four novel objects placed around the perimeter. The test mouse is allowed to freely explore the arena and the order in which the mouse investigates the novel objects is recorded. From these data, patterned sequences of exploration can be identified, as can the most preferred object for each mouse. The representative data shared here and past results using the novel object exploration assay illustrate that inbred mouse strains do demonstrate different behavior in this assay and that strains with elevated lower order RRBs also show elevated patterned behavior. As such, the novel object exploration assay appears to possess good face validity for higher order RRBs in humans and may be a valuable assay for future studies investigating novel therapeutics for ASD.

Higher order restricted, repetitive behaviors (RRBs) disrupt the lives of affected individuals. These behaviors are challenging to model in rodents, making basic biomedical research into potential treatments or interventions for RRBs difficult. Here we describe novel object exploration as a potential assay for higher order RRBs in mice.

Restricted, repetitive behaviors (RRBs) are a core feature of autism spectrum disorder (ASD) and disrupt the lives of affected individuals.  RRBs are ...

A Behavioral Assay for Investigating the Role of Spatial Memory During Instinctive Defense in Mice

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often stereotyped actions elicited in response to innately aversive sensory stimuli, but their success requires enough flexibility for adapting to different spatial environments, which can change rapidly. Here, we describe a behavioral assay to evaluate the influence of learned spatial knowledge on defensive behaviors in mice. We have adapted the widely used Barnes maze spatial memory assay to investigate how mice navigate to a shelter during escape responses to innately aversive sensory stimuli in a novel environment, and how they adapt to acute changes in the environment. This new assay is an ethological paradigm that does not require training and exploits the natural exploration patterns and navigation strategies in mice. We propose that the set of protocols described here are a powerful means of studying goal-directed behaviors and stimulus-triggered navigation, which should be of interest to both the fields of instinctive behaviors and spatial memory.

This protocol describes a behavioral assay, based on the Barnes maze test, to study how instinctive defensive actions are modified by the knowledge of spatial environment.

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often ...

Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms

The Barnes maze is a reliable measure of spatial learning and memory that does not require food restriction or exposure to extremely stressful stimuli. The Barnes maze can also assess other mouse behaviors, such as general motivation to escape from the maze platform and exploratory behavior. The Barnes maze can measure whether a genetic mutation or environmental variable can impact the acquisition and retention of spatial memories, as well as provide information about the search strategy employed by the mice. Here we use the Barnes maze to detect a memory deficit in adult mice following a single developmental ethanol exposure event. The newly described Damsel-in-Distress paradigm exposes a male mouse to a female mouse trapped in a chamber in the open center field of the arena. It provides an opportunity for the mouse to socially respond to the trapped female and exhibit prosocial behavior. The Damsel-in-Distress paradigm can also be used to examine mouse behavior in a novel arena and measure locomotor activity. Both the Barnes Maze and the Damsel-in-Distress protocols require minimal financial investment and most aspects of the tests can be constructed from common lab supplies. These flexible and accessible tools can also be used to detect behavioral changes over the course of development.

This protocol measures spatial learning and memory using the Barnes maze. A novel Damsel-in-Distress paradigm is used to assess locomotor activity and prosocial behavior in mice.

The Barnes maze is a reliable measure of spatial learning and memory that does not require food restriction or exposure to extremely stressful stimuli. ...

Validating a Murine Model of Angelman Syndrome Using Behavioral Tests

Behavioral Characterization of an Angelman Syndrome Mouse Model

This manuscript describes a battery of behavioral tests available to characterize Angelman syndrome (AS)-like phenotypes in an established murine model of AS. We use the rotarod learning paradigm, detailed gait analysis, and nest building test to detect and characterize animal motor impairments. We test animal emotionality in the open field and elevated plus maze tests, as well as the affect in the tail suspension test. When AS mice are tested in the open field test, the results should be interpreted with care, since motor dysfunctions influence mouse behavior in the maze and alter activity scores.
The reproducibility and effectiveness of the presented behavioral tests has already been validated in several independent Uba3a mouse lines with different knockout variants, establishing this set of tests as an excellent validation tool in AS research. Models with the relevant construct and face validity will warrant further investigations to elucidate the pathophysiology of the disease and grant the development of causal treatments.

Author Spotlight: A Battery of Highly Reproducible Behavioral Tests to Validate an Angelman Syndrome Murine Model 

This manuscript presents a set of highly reproducible behavioral tests to validate an Angelman syndrome mouse model.

This manuscript describes a battery of behavioral tests available to characterize Angelman syndrome (AS)-like phenotypes in an established murine model of ...

Behavioral Assays for Autism Spectrum Disorder in Mouse Models

Strategies for Assessing Autistic-Like Behaviors in Mice

Autism spectrum disorder (ASD) is a neurobiologically complex condition with a heterogeneous genetic etiology. Clinically, ASD is diagnosed by social communication impairments and restrictive or repetitive behaviors, such as hand flapping or lining up objects. These behavioral patterns can be reliably observed in mouse models with ASD-linked genetic mutations, making them highly useful tools for studying the underlying cellular and molecular mechanisms in ASD. Understanding how genetic changes affect the neurobiology and behaviors observed in ASD will facilitate the development of novel targeted therapeutic compounds to ameliorate core behavioral impairments. Our lab has employed several protocols encompassing well-described training and testing procedures that reflect a wide range of behavioral deficits related to ASD. Here, we detail two assays to study the core features of ASD in mouse models: self-grooming (a measure of repetitive behavior) and the three-chamber social interaction test (a measure of social interaction approach and preference for social novelty).

Rodent models are valuable tools for studying core behaviors related to autism spectrum disorder (ASD). In this article, we expound on two behavioral tests for modeling the core features of ASD in mice: self-grooming, which assesses repetitive behavior, and the three-chamber social interaction test, which documents social impairments.

Autism spectrum disorder (ASD) is a neurobiologically complex condition with a heterogeneous genetic etiology. Clinically, ASD is diagnosed by social ...

Modeling Early-Life Adversity in Mice Using Limited Bedding and Nesting

Limited Bedding and Nesting as a Model for Early-Life Adversity in Mice

Early-life adversity (ELA), such as abuse, neglect, lack of resources, and an unpredictable home environment, is a known risk factor for developing neuropsychiatric disorders such as depression. Animal models for ELA have been used to study the impact of chronic stress on brain development, and typically rely on manipulating the quality and/or quantity of maternal care, as this is the major source of early-life experiences in mammals, including humans. Here, a detailed protocol for employing the Limited Bedding and Nesting (LBN) model in mice is provided. This model mimics a low-resource environment, which provokes fragmented and unpredictable patterns of maternal care during a critical developmental window (postnatal days 2-9) by limiting the amount of nesting materials given to the dam to build a nest for her pups and separating the mice from the bedding via a mesh platform in the cage. Representative data are provided to illustrate the changes in maternal behavior, as well as the diminished pup weights and long-term changes in basal corticosterone levels, that result from the LBN model. As adults, offspring reared&#160;in the LBN environment have been shown to exhibit an aberrant stress response, cognitive deficits, and anhedonia-like behavior. Therefore, this model is an important tool to define how the maturation of stress-sensitive brain circuits is altered by ELA and results in long-term behavioral changes that confer vulnerability to mental disorders.

Author Spotlight: Exploring Microglial Interactions with Stress-Response Circuitry Using the Limited Bedding and Nesting Model

This protocol describes an animal model for studying how early-life adversity, provoked by an impoverished environment and unpredictable maternal care during the early postnatal period, affects brain development and the future risk of mental disorders.

Early-life adversity (ELA), such as abuse, neglect, lack of resources, and an unpredictable home environment, is a known risk factor for developing ...

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

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