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Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

21.3K Views

09:43 min

November 30th, 2018

DOI :

10.3791/58648-v

November 30th, 2018


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H3 Histone

Hoofdstukken in deze video

0:04

Title

1:06

Preparation of the Sample Extract

2:38

Preparation of the Crude Histone Extract

3:33

Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract

4:14

Purification of Core Histones

5:13

Precipitation of Core Histones

7:02

Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain

9:02

Conclusion

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