JoVE Journal

Biochemistry

Author Produced

This content is Open Access.

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

This protocol demonstrates a simple, robust and high throughput single molecule flow-stretching assay for studying one-dimensional (1D) diffusion of molecules along DNA.

Hoofdstukken in deze video

0:00

Title

0:15

Introduction

1:31

Preparation of the biotin-λ-DNA

2:52

Coverslip functionalization

4:10

Flow cell construction

5:44

TIRF Imaging

8:15

Data Analysis

11:41

Conclusion

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