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In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

14.2K Views

18:48 min

August 12th, 2017

DOI :

10.3791/55510-v

August 12th, 2017


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Transplant

Hoofdstukken in deze video

0:00

Title

0:37

LEW.1W to LEW.1A Allograft Procedure

2:18

Isolation of Allograft APCs (pDCs)

6:12

Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)

10:30

CFSE Labeling of Responder Cells to Measure their Proliferation

12:09

FACS Staining and Analysis After 6 Days of Coculture

13:49

In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient

16:33

Representative Results

18:03

Conclusion

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