JoVE Journal

Genetics

This content is Open Access.

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

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09:34 min

September 20th, 2016

September 20th, 2016


An improved polymerase chain reaction-restriction fragment length polymorphism method for genotyping pufferfish species by liquid chromatography/mass spectrometry is described. A reverse-phase silica monolith column is employed for separating digested amplicons. This method can elucidate the monoisotopic masses of oligonucleotides, which is useful for identifying base composition.

Hoofdstukken in deze video

0:05

Title

0:45

DNA Extraction

2:58

PCR and Enzymatic Digestion

4:32

Liquid Chromatography/Electrospray Ionization Mass Spectrometry (LC/ESI-MS) Analysis

7:30

Results: Representative Results of the Analysis of Raw Muscle from Takifugu rubripes

8:44

Conclusion

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