JoVE Journal

Immunology and Infection

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Nanyang Technological University

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

Hoofdstukken in deze video

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Title

0:10

Introduction

1:14

Animation

2:37

Washing

3:22

Antigen Challenge

3:48

Substrate

4:23

Stopping the Reaction

4:47

Reading

4:54

Calculations

6:22

Results

7:02

Conclusion

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