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Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

14.5K Views

15:12 min

May 10th, 2014

DOI :

10.3791/51447-v

May 10th, 2014


Transcript

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Keywords Cystic Fibrosis

Hoofdstukken in deze video

0:05

Title

1:46

Solubilization of Microsomes

3:49

Nickel-affinity Purification of CFTR

5:33

Gel Permeation Chromatography (GPC) Purification of CFTR

6:57

Reconstitution of CFTR

7:51

Measurement of ATPase Activity

9:35

Results: Isolation of Milligram Quantities of CFTR by Two Methods

14:28

Conclusion

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