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Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

33.5K Views

12:03 min

January 22nd, 2014

DOI :

10.3791/51295-v

January 22nd, 2014


Transcript

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Keywords Recombinant Protein Engineering

Hoofdstukken in deze video

0:05

Title

2:55

Bacterial Cell Culture

4:25

Isolation and Purification of Recombinant Protein

5:42

Ni-NTA Affinity Chromatography

8:31

Results: Purification of Native and Nonnative Proteins for Biotinylation

10:32

Conclusion

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