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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

7.1K Views

10:33 min

January 8th, 2014

DOI :

10.3791/51023-v

January 8th, 2014


Transcript

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Keywords Affinity Chromatography

Hoofdstukken in deze video

0:05

Title

1:39

Preparation and Loading of Protein A Membrane Adsorber

3:36

Eluting, Concentrating, and Dialyzing the Protein

4:49

Quantify and Characterize Purified Product in an Expedited Western Blotting Procedure

7:00

Results: Characterization of Chimeric Murine Galectin-1 Fused to Human Fc (Gal-1hFc)

10:11

Conclusion

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