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Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

24.3K Views

10:32 min

March 4th, 2013

DOI :

10.3791/50215-v

March 4th, 2013


Transcript

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Keywords Mitochondria associated ER Membranes MAMs

Hoofdstukken in deze video

0:05

Title

2:21

Subcellualar Fractionation: Removal of Nuclei, Unlysed Cells, and Cellular Debris

4:08

Crude Mitochondria Isolation

6:16

Isolation of Mitochondria-associated ER Membrane, MAMs

8:27

Extraction of Glycosphingolipid-enriched Microdomains, GEMs

9:14

Results: Western Blot Analysis of GEMs and MAMs

10:01

Conclusion

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