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Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

14.1K Views

10:34 min

February 1st, 2013

DOI :

10.3791/50156-v

February 1st, 2013


Transcript

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Keywords Sleeping Beauty Transposon

Hoofdstukken in deze video

0:05

Title

1:00

Tumor Collection and DNA Isolation

2:16

Digesting and Ligating Linkers to the DNA

5:51

Removing Concatamer Transposons Using a Second Digestion

6:54

Amplifying the Tumor DNA

9:19

PCR and Sequencing Results

10:08

Conclusion

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