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Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

35.8K Views

16:33 min

February 18th, 2013

DOI :

10.3791/50031-v

February 18th, 2013


Transcript

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Keywords Protein Palmitoylation

Hoofdstukken in deze video

0:05

Title

2:08

Extraction of Target Protein from Cultured Primary Hippocampal Neurons

5:54

Acyl-Biotin Exchange: Hydroxylamine (HAM) Cleavage

9:17

Elution of Biotin-conjugated Target Protein and SDS-PAGE

10:46

Western Blotting and Detection of Palmitoylation of Target Protein

12:16

Results: Detecting Palmitoylation of a Neuronal Protein

16:07

Conclusion

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