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Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species

13.7K Views

14:33 min

August 30th, 2012

DOI :

10.3791/4132-v

August 30th, 2012


Transcript

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Protein Aggregation

Hoofdstukken in deze video

0:05

Title

2:47

Preparation of Aggresome Donor Cells: Transfection of mRFP- or eGFP-tagged Human Full-length DISC1

3:50

Aggresome Purification from Transfected Donor Cells

6:43

Treatment of Recipient SH-SYBY Cells with mRFP-DISC1 Aggresomes

8:13

Labeling of Recombinant DISC1(598-785) with DyLight594

10:44

Treatment of Recipient SH-SY5Y Cells with Labeled Recombinant DISC1(598-785) Protein

12:00

Representative Invasion Assay Results

13:30

Conclusion

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