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Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

14.7K Views

07:20 min

January 14th, 2011

DOI :

10.3791/2381-v

January 14th, 2011


Transcript

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Ex Vivo Expansion

Hoofdstukken in deze video

0:05

Title

0:55

Introduction

1:48

Mouse Model of Breast Cancer

2:14

Isolation of Lymphocytes

3:44

Determine Fold Expansion of T Cells by Cell Counts and Flow Cytometry Analysis

4:58

Determine Tumor-Specificity of the ex vivo Expanded T Cells

5:47

Selective Expansion of Tumor-Reactive T Cells with Effector Anti-Tumor Function

6:53

Conclusion

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