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In vivo and in vitro Studies of Adaptor-clathrin Interaction

14.5K Views

17:14 min

January 26th, 2011

DOI :

10.3791/2352-v

January 26th, 2011


Transcript

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In Vivo

Hoofdstukken in deze video

1:34

Introduction

2:10

Generation of GFP-tagged Sla1 and Sla1 Clatherin-binding Mutant Yeast Strains

3:22

Fluorescence Microscopy

5:09

Fluorescence Image Analysis

6:41

Preparation of Yeast Cytosolic Extract

9:54

GST-fusion Protein Affinity Assay

11:53

Preparation of Total Cell Extract

13:36

Co-immunoprecipitation Assay

15:16

Representative Results

16:39

Conclusion

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