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Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

13.4K Views

12:04 min

December 1st, 2009

DOI :

10.3791/1599-v

December 1st, 2009


Transcript

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Isolation

Hoofdstukken in deze video

0:00

Title

0:58

Introduction

1:31

Selecting and Washing Larvae

2:27

Dissecting the Larvae and Removing Non-Specific Cells

4:47

Dissociating the Tissue into a Single Cell Suspension

8:07

Magnetic Bead Cell Sorting

10:05

Representative Results of DA Neurons Isolation and RNA Extraction

11:15

Conclusion

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