A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress ExposureJenny L. Carey 1, Olivia H. Cox 1, Fayaz Seifuddin 1, Leonard Marque 1, Kellie L.K. Tamashiro 1, Peter P. Zandi 1,3, Gary S. Wand 1,2, Richard S. Lee 1
1Departments of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, 2Department of Medicine, Johns Hopkins School of Medicine, 3Department of Mental Health, Johns Hopkins School of Public Health
Here, we describe the protocol and implementation of Methyl-Seq, an epigenomic platform, using a rat model to identify epigenetic changes associated with chronic stress exposure. Results demonstrate that the rat Methyl-Seq platform is capable of detecting methylation differences that arise from stress exposure in rats.
