Tianjin Medical University

7 ARTICLES PUBLISHED IN JoVE

Chemistry

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Li Xie¹, Liqin Chen³, Pan Gu⁴, Lanlan Wei¹, Xuejun Kang²

¹School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, ²Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, ³School of Public Health, Tianjin Medical University, ⁴British Columbia Academy, Nanjing Foreign Language School

We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.

Medicine

Induction and Micro-CT Imaging of Cerebral Cavernous Malformations in Mouse Model

Jaesung P. Choi^1,2, Xi Yang³, Matthew Foley⁴, Xian Wang¹, Xiangjian Zheng^1,2,3

¹Lab of Cardiovascular Signaling, Centenary Institute, ²Faculty of Medicine, Sydney Medical School, University of Sydney, ³Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, ⁴Australian Centre for Microscopy & Microanalysis, University of Sydney

This protocol demonstrates the induction of cerebral cavernous malformation disease in a mouse model and uses contrast enhanced micro computed tomography to measure lesion burden. This method enhances the value of established mouse models to study the molecular basis and potential therapies for cerebral cavernous malformation and other cerebrovascular diseases.

Medicine

Full-Endoscopic Isolation Zone Technique for the Treatment of Lumbar Disc Herniation

Lu Wang^*1, Lingxia Li^*2, Cai Cheng¹, Zhiyuan Guo¹, Dasen Xin¹, Xinbiao Yan¹, Weibin Li¹, Hongmin Han¹

¹Department of Orthopedics, Cangzhou Central Hospital, Tianjin Medical University, ²Department of Clinical Pharmacy, People's Hospital of Cangzhou City

Here, we present a protocol for the "isolation zone" technique in the treatment of lumbar disc herniation (LDH) under full-endoscopic spine surgery (FESS), including intervertebral foramen formation, targeted catheterization, nucleus pulposus resection, and annulus fibrosus formation, which together completely block the pain from the nerve conduction pathway.

Biology

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay

Yuping Fan^*1,2, Yan Teng^*3, Fu tong Liu^*4, Fengxia Ma^1,2, Alan Y. Hsu⁵, Sizhou Feng^1,2, Hongbo R. Luo⁵

¹State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, ²Tianjin Institutes of Health Science, ³Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, ⁴Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, ⁵Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center

This protocol details the preparation of CLON-G to extend the neutrophil lifespan to greater than 5 days and provides a reliable procedure for evaluating neutrophil death with flow cytometry and confocal fluorescence microscopy.

Cancer Research

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

Xiangqin Zhao¹, Xinbo Gao¹, Wenwen Yu², Yi Lu¹, Xuesong Li¹, Lu Tan³, Xiubao Ren², Yifei Wang¹, Weijie Song⁴, Jihui Hao¹, Ying Ma¹

¹Department of Pancreatic Cancer, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin Key Laboratory of Digestive Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, ²Department of Immunology and Biotherapy, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Immunology and Biotherapy, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, ³Research Core Facility, School of Basic Medical Sciences, Tianjin Medical University, ⁴Department of Public Laboratory, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital

We describe a method to utilize tumor-infiltrating lymphocytes (TILs) from mice through flow cytometry for adoptive cell transfer. This protocol aims to verify the specific cytotoxicity of TILs against tumors in a syngeneic pancreatic cancer mouse model, providing insights into the development of adoptive cell therapies for pancreatic cancer.

Biology

Isolation and Culture of Primary Cholangiocytes from Mice with Polycystic Liver Disease Using a Two-step Digestion Method

Rongjie Ji¹, Bo Qing², Jiayuan Chen², Zhigang Zhao¹, Lirong Zhang²

¹Department of Medical Oncology, Tianjin First Central Hospital, School of Medicine, Nankai University, ²The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), State Key Laboratory of Experimental Hematology, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University

This protocol describes an optimized two-step digestion method for isolating high-purity and high-viability primary cholangiocytes from wild-type mice and mice with polycystic liver disease.

Medicine

Murine Model of Thoracic Aortic Dissection Induced by Oral β-Aminopropionitrile and Subcutaneous Angiotensin II Infusion

Yi Zhang¹, Chunyan Wu², Chunxiao Wan³, Naishi Wu¹, Shengkai Zuo^1,2

¹Department of Cardiovascular Surgery, Tianjin Medical University General Hospital, ²Department of Biopharmaceutics, Tianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, ³Department of Physical and Rehabilitation Medicine, Tianjin Medical University General Hospital

This protocol provides a detailed, step-by-step procedure for the induction of thoracic aortic dissection in mice. Specifically, it includes the precise calculation of the required doses of β-aminopropionitrile and angiotensin II, the procedure for osmotic pump filling, and the implantation technique of the osmotic pump.