CD Laboratory for Next Generation CAR T Cells

1 ARTICLES PUBLISHED IN JoVE

Protein Engineering by Yeast Surface Display

Charlotte U. Zajc^1,2, Elise Sylvander^2,3, Magdalena Teufl^1,2, Michael W. Traxlmayr^1,2

¹Department of Chemistry, Institute of Biochemistry, BOKU University, ²CD Laboratory for Next Generation CAR T Cells, ³St. Anna Children's Cancer Research Institute, CCRI

This protocol describes the essential steps for conducting yeast surface display selection campaigns to enrich protein variants binding to an antigen of interest.