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1 ARTICLES PUBLISHED IN JoVE

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Biology

A Validatable Droplet Digital Polymerase Chain Reaction Assay for the Detection of Adeno-Associated Viral Vectors in Bioshedding Studies of Tears
Bryanna Longacre 1, Amanda Souaysene 1, Carrie A. Vyhlidal 1, Matthew R. Pennington 1
1Cell and Gene Therapy, KCAS Bioanalytical and Biomarker Services

Here, we present a protocol for the development and validation of good laboratory practices in the compliant detection of adeno-associated viral vectors in human tears by droplet digital polymerase chain reaction in support of clinical development of gene therapy vectors.

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