近接ライゲーションアッセイを用いた異流体せん断応力条件下でのTGFβ/BMP/SMADシグナル伝達の可視化と定量化

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Introduction

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Cell Culture and Fluid Shear Stress Exposure

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Fixation

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Blocking and Incubation with Primary Antibody

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Incubation with PLA Probe and Ligation

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Amplification and Mounting

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Results: Effect of Shear Stress on the SMAD Protein Interactions

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Conclusion

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