JoVE Journal

Developmental Biology

This content is Open Access.

Temporale ordinazione di espressione Dynamic Data di espressione spaziale dettagliata Mappe

The segmentation clock drives oscillatory gene expression across the pre-somitic mesoderm (PSM). Dynamic Notch activity is key to this process. We use imaging and computational analyses to extract temporal dynamics from spatial expression data to demonstrate that Delta ligand and Notch receptor expression oscillate in the vertebrate PSM.

Capitoli in questo video

0:05

Title

0:50

PSM Explant Dissection

2:49

Immunohistochemistry of PSM Explants

4:38

Fluorescent In Situ Hybridization (FISH) of PSM Explants

7:09

Prepare Samples for Imaging

8:33

Results: Spatiotemporal Expression Profiles for Dll1 and Notch1 According to Expression of the Clock Gene Lfng

10:21

Conclusion

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