Registrazione neuronale attività Temperatura indotta attraverso il monitoraggio dei cambiamenti di calcio nel bulbo olfattivo di

Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.