Caratterizzazione metabolica di Polarized M1 e M2 macrofagi derivate dal midollo osseo Utilizzando analisi in tempo reale extracellulare Flux

Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.