JoVE Journal

Developmental Biology

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Nanyang Technological University

בידוד מהיר של BMPR-IB + השומן שמקורם בתאי סטרומה לשימוש במודל ריפוי פגם calvarial

Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

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Chapters in this video

0:05

Title

1:16

Harvesting and Isolation of ASCs

3:19

FACS Sorting for BMPR-1B Positive Cells

5:39

Creation of Calvarial Defects and Application of ACS-containing Scaffold

7:03

Results: Bone Regeneration in Response to BMPR-1B-positive ASC

8:02

Conclusion

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