JoVE Journal

Developmental Biology

This content is Open Access.

דור של אגרגטים של תאי עכבר גזע עובריים שצג שבירת סימטריה, קיטוב והתנהגות קולקטיבית מתהווה

We have developed a protocol to generate aggregates of mouse embryonic stem cells that display self-organization, symmetry breaking and elongation paralleling axial development. This technique allows the study of axial developmental processes and the generation of cell types that are otherwise difficult to perform in monolayer culture.

Chapters in this video

0:05

Title

0:39

Cell Culture and Generation of Aggregates

3:12

Stimulation of Cells in Aggregates

4:29

Cell Aggregate Fixation, Permeabilization and Blocking

7:05

Antibody Binding and Detection

9:53

Results: Morphological Evaluations of mESC Aggregate Culture

10:57

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More