JoVE Journal

Behavior

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Nanyang Technological University

ניתוח מקיף של תמלול Dynamics מדוגמאות המוח בעקבות ניסיון התנהגות

This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.

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Chapters in this video

0:05

Title

1:10

Handling and Cocaine Exposure

2:27

Dissection of the Nucleus Accumbens

3:47

RNA Extraction

4:46

qPCR Analysis

7:04

Results: Transcription Dynamics in the Nucleus Accumbens after Cocaine Exposure

7:51

Conclusion

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