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הדמיה ברזולוציה סופר של Cytokinetic Z הטבעת בחיידקים חיים באמצעות-Structured 3D מהיר תאורה מיקרוסקופית (f3D-SIM)

Spatiotemporal information about dynamic proteins inside live cells is crucial for understanding biology. A type of super-resolution microscopy called fast 3D-structured illumination microscopy (f3D-SIM) reveals unique information about the cytokinetic Z ring in bacteria: both its bead-like appearance and the rapid dynamics of FtsZ within the ring.

Chapters in this video

0:05

Title

2:04

Sample Preparation

4:05

Preparation of the Microscope

5:01

Image Acquisition

6:44

Checking Data Quality and Reconstructing 3D-SIM Images

8:01

Generating 3D Intensity Plots of the Z Ring from 3D-SIM Image Data

9:58

Results: OMX Fast 3D-SIM to Investigate FtsZ-GFP Localization and Dynamics in Live Bacillus subtilis

11:39

Conclusion

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