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Nanyang Technological University

Los enfoques experimentales para estudiar mitocondrial localización y función de un ciclo celular quinasa nuclear, Cdk1

Here, we outline how to study mitochondrial localization of a (cell cycle) kinase, and how to determine its sub-mitochondrial location as well as potential mitochondrial substrates/targets. Forced expression of proteins into the mitochondria provides a useful tool for studying the functional consequences of mitochondrial localization of a protein of interest.

Capítulos en este video

0:05

Title

1:01

Sodium Carbonate Extraction of Intact Mitochondria

3:07

Separation of Inner and Outer Membranes of Mitochondria

4:22

Construction of Mitochondria-targeted GFP/RFP-tagged CyclinB1/Cdk1 Vectors and Mitochondria Localization

7:23

Determination of Cell Cycle Phase Lengths with EdU Incorporation Assay

10:58

Results: Mitochondrial Analysis of CyclinB1 and Cdk1

12:25

Conclusion

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