Verbesserte Sample Multiplexing von Gewebe mit Combined Precursor Isotopenmarkierung und Isobaric Tagging (cPILOT)

1:14

Protein Extraction and Generation of Peptides for Chemical-tagging

2:05

Sample Digestion

3:16

Dimethylation Labeling (N-termini)

5:13

Isobaric Tagging (Lys residues)

6:12

Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS) and MS³

7:02

Results: cPILOT Can Be Used to Analyze Tissues from an Alzheimer's Disease Mouse Model and Wild-type Controls

8:23

Conclusion