JoVE Journal

Engineering

This content is Open Access.

Protonen-Transfer und Protein-Zustände Dynamics in Lichtempfindliche Proteine, die durch zeitaufgelöste Step-Scan Fourier-Transformations-Infrarot-Spektroskopie

Key steps of protein function, in particular backbone conformational changes and proton transfer reactions, often take place in the microsecond to millisecond time scale. These dynamical processes can be studied by time-resolved step-scan Fourier-transform infrared spectroscopy, in particular for proteins whose function is triggered by light.

Kapitel in diesem Video

0:05

Title

2:13

Preparation of Attenuated Total Reflection Experiments on Bacteriorhodopsin

3:48

Performing Transmission Experiments on Detergent-solubilized Channelrhodopsin-2

5:02

Adjustment and Synchronization of the Exciting Laser

5:55

Time-resolved Step-scan Preparations and Settings

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Results: Time-resolved Step-scan FTIR Experiments to Determine Protonation and Protein Conformational Changes

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Conclusion

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