人精切肺切片中物质的细胞毒性及免疫调节作用的评价

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13.6K Views

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12:04 min

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May 9th, 2018

DOI :

10.3791/57042-v

May 9th, 2018

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Vanessa Neuhaus*¹, Olga Danov*¹, Sebastian Konzok¹, Helena Obernolte¹, Susann Dehmel¹, Peter Braubach², Danny Jonigk², Hans-Gerd Fieguth³, Patrick Zardo⁴, Gregor Warnecke⁴, Christian Martin⁵, Armin Braun¹^,⁶, Katherina Sewald¹

¹Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, ²Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ³Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), ⁴Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ⁵Institute of Pharmacology and Toxicology, RWTH Aachen University, ⁶Institute for Immunology, Hannover Medical School

摘要

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此视频中的章节

0:05

Title

1:26

General Preparation of Human PCLS and Subsequent Exposure to Chemicals

4:28

Measurement of Chemically-induced Cytotoxicity in Human PCLS by WST-1 Assay

6:01

LDH Assay

7:29

Microscopic Viability Assay with Confocal Laser Scanning Microscope (cLSM)

9:49

Results: Representative Images of the Three-dimensional Microscopic Staining and Image Rendering of Human PCLS

10:59

Conclusion

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