Name: using the sleeve technique in a mouse model of aortic transplantation - an instructional video
Uploaded: 2017-10-22T13:00:00.000+00:00
Duration: 8 min 55 s
Description: Watch this Scientific Journal Video about Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video at JoVE.com

我们要感谢罗马维乔雷克和彼得 Kurdybacha 的出色编辑协助, 以及里昂. 德克尔和乌利 Heuter 的出色技术援助。

涉及动物的程序由 RWTH 亚琛大学的机构动物护理和使用委员会 (IACUC) 批准, AZ 84-02.04.2012. A234. 
 注意: 使用 CD1 背景的成年雄性野鼠演示了该过程。在手术前后将小鼠放在一个专门的实验室里, 确保适当地获得食物、专门的兽医控制和治疗。如果这些动物是从外面买来的, 在做手术前允许一周的驯化. 
 1. 捐赠者的准备 <ol> <li> 使用无菌材料和器械在手术期间保持无菌状态以避免感染. </li>
	<li> 麻醉每只老鼠用1.5% 的混合异氟醚和100% 氧气通过口罩。将鼠标放在仰卧位的平台上, 并将其所有的腿粘在手术台上。通过捏后腿来检查其反射, 以确保鼠标足够麻醉。将眼部药膏放在眼部, 以防在手术过程中干燥. </li>
	<li> 用脱毛凝胶或剃须刀从腹部取出所有毛发。在无菌条件下进行操作。用洗必泰和无菌水交替擦洗腹部消毒. </li>
	<li> 通过带剪刀或手术刀的中线腹切口移除供体主动脉。将肠道手动缩回右侧。用无粉手套轻轻地将肠道反射到一侧. 
	<ol> <li> 将肠道放在一块用盐水浸湿的纱布上, 以保持其湿润. </li>
		<li> 用镊子将腹主动脉从周围组织中仔细解剖. </li>
		<li> 用镊子将大动脉之间的动脉段与腔静脉分叉分开. </li>
		<li> 使用11-0 单丝单线缝合, 确保此段的所有小分支都非常小心. </li>
		<li> 在移除主动脉之前, 将含 50 U 肝素的盐水溶液注入0.5 毫升 (毫升) 到下腔静脉. </li>
		<li> 让供体动物血在主动脉段后被移除. </li>
		<li> 将移植物完全用盐水冲洗干净, 然后立即将其转移到 ice-cold 盐水的容器中. </li>
	</ol> </li> </ol> 2。准备收件人 <ol> <li> 麻醉1.5% 的混合体, 由异氟醚和100% 氧气通过面罩, 然后删除头发和消毒 (1 节) 的受体动物。用手术刀将中线切开, 从剑到骨盆, 并缩回腹壁。将眼部药膏放在眼部上, 防止在手术过程中出现干燥. </li>
	<li> 在盐水溶液中用湿纱布包裹肠道, 并轻轻地移到动物和 #39; 右. </li>
	<li> 解剖下主动脉游离于肾动脉下部与镊子远端分岔之间. </li>
	<li> 使用11-0 单丝单线缝合, 确保此段的所有小分支都非常小心. </li>
	<li> 用6-0 单丝缝合钳夹住主动脉的近端和远端部分. </li>
	<li> 在夹钳中间划分主动脉, 用素盐水冲洗切口末端, 使管腔张开. </li>
	<li> 将移植物放在原位, 将喂食容器端插入接收容器, 然后用11-0 单丝进行缝合, 以避免主动脉的任何扭转, 适当地对准施主和接受者 ( 图 1 ) 10 . </li> <li> 在对吻合进行检查后, 仔细地松开绷带。先松开远端钳。这导致在释放近端高压侧之前, 将墙壁压在一起的低压. </li>
	<li> 灌注立即进行移植, 并检查是否有可见的脉搏。轻轻地除去丝绸的残余。供体和受体主动脉的最佳重叠长度为 1-2 mm. </li>
	<li> 将腹内内容返回腹腔, 并用3-0 聚酸缝合闭合整个伤口. </li>
	<li> 在终止麻醉前给小鼠丁丙诺啡 (0.05 毫克/千克体重皮下 (SC)). </li>
	<li> 在完全清醒之前, 不要把动物留在无人看管的状态。管理疼痛治疗与 buprenophine 0.05 毫克/千克体重管理 SC 三次, 每天三天的行动后, 经机构监督机构批准. </li>
	<li> 用于组织捕捞, 麻醉上述的受体小鼠, 用磷酸盐缓冲盐水 (pbs) 冲洗血管, 然后用4% 甲醛/pbs (pH = 7.4) 通过心脏穿刺。轻轻取出移植物。经过隔夜固定4% 甲醛/PBS, 过程标本进一步和嵌入石蜡. </li>
</ol>

<ol>
	<li>Rowinska, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-+invasive+in+vivo+analysis+of+a+murine+aortic+graft+using+high+resolution+ultrasound+microimaging.">Non- invasive in vivo analysis of a murine aortic graft using high resolution ultrasound microimaging.</a> Eur J Radiol. 81 (2), 244-249 (2012).</li><li>Daugherty, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+atherosclerosis.">Mouse models of atherosclerosis.</a> Am J Med Sci. 323 (1), 3-10 (2002).</li><li>Xu, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+arteriosclerosis:+from+arterial+injuries+to+vascular+grafts.">Mouse models of arteriosclerosis: from arterial injuries to vascular grafts.</a> Am J Pathol. 165 (1), 1-10 (2004).</li><li>Daugherty, A., Cassis, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+abdominal+aortic+aneurysms.">Mouse models of abdominal aortic aneurysms.</a> Arterioscler Thromb Vasc .Biol. 24 (3), 429-434 (2004).</li><li>Zernecke, A., Shagdarsuren, E., Weber, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemokines+in+atherosclerosis:+an+update.">Chemokines in atherosclerosis: an update.</a> Arterioscler Thromb Vasc Biol. 28 (11), 1897-1908 (2008).</li><li>Chereshnev, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+model+of+heterotopic+aortic+arch+transplantation.">Mouse model of heterotopic aortic arch transplantation.</a> J Surg Res. 111 (2), 171-176 (2003).</li><li>Koulack, J., McAlister, V. C., Giacomantonio, C. A., Bitter-Suermann, H., MacDonald, A. S., Lee, T. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+mouse+aortic+transplant+model+of+chronic+rejection.">Development of a mouse aortic transplant model of chronic rejection.</a> Microsurgery. 16 (2), 110-113 (1995).</li><li>Engelbrecht, G., Kahn, D., Duminy, F., Hickman, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+rapid+technique+for+renal+transplantation+in+the+rat.">New rapid technique for renal transplantation in the rat.</a> Microsurgery. 13 (6), 340-344 (1992).</li><li>Baxter, K., Hao, P. M., Howden, B. O., Saunder, A., Jablonski, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modified+technique+of+abdominal+heart+transplantation+in+the+rat.">Modified technique of abdominal heart transplantation in the rat.</a> J Heart Lung Transplant. 17 (11), 1057-1064 (1998).</li><li>Dambrin, C., Calise, D., Pieraggi, M. T., Thiers, J. C., Thomsen, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+aortic+transplantation+in+mice:+a+new+model+of+allograft+arteriosclerosis.">Orthotopic aortic transplantation in mice: a new model of allograft arteriosclerosis.</a> J Heart Lung Transplant. 18 (10), 946-951 (1999).</li><li>Siemionow, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histopathology+of+microarterial+anastomoses:+end-to-end+versus+end-in-end+(sleeve)+technique.">Histopathology of microarterial anastomoses: end-to-end versus end-in-end (sleeve) technique.</a> J Hand Surg Am. 15, 619-625 (1990).</li><li>Charo, I. F., Ransohoff, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+many+roles+of+chemokines+and+chemokine+receptors+in+inflammation.">The many roles of chemokines and chemokine receptors in inflammation.</a> N Engl J Med. 354 (6), 610-621 (2006).</li><li>Sun, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+surgical+technique+for+the+establishment+of+a+murine+model+of+aortic+transplantation.">Improved surgical technique for the establishment of a murine model of aortic transplantation.</a> Microsurgery. 18 (6), 368-371 (1998).</li><li>Guo, L., Agarwal, A., George, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+aortic+transplantation+in+mice+for+the+study+of+vascular+disease.">Orthotopic aortic transplantation in mice for the study of vascular disease.</a> J Vis Exp. (69), e4338 (2012).</li></ol>

作者声明他们没有竞争的金融利益。

在本研究之前, 对小鼠其他各种移植模型进行了全面分析3,6,7,10,13,14。采用 Dambrin et al.修饰的袖状技术进行主动脉移植的模型选择, 因为它符合我们的标准, 并显示了与传统的端部缝合方法相比, 套管吻合的高可靠性1,10。
这种技术在许多方面都是有利的, 阻断时间大大减少了手术期间对主动脉损伤的最小化。此外, 还观察到血栓形成率低的情况, 以避免捐赠者和受赠人之间的血管口径可能不匹配。上述观察使该技术非常适合于研究小鼠主动脉移植中的血管疾病。
在一项随访研究中, 超声在移植后8周内进行, 未发现明显的变化。这证实了在手术中对主动脉的任何损伤都是最小的1。
本文提出的嫁接过程保证了移植物完整性和功能的不损害。因此, 这一实验性移植模型可以作为对基因工程小鼠退行性血管病的分子和药理学研究的重要工具。
我们认为, 视频指南可以作为教学材料, 说明使用这个简单的动动脉模型, 它将有助于进一步丰富的辩论中的许多重要问题的血管病理。这种快速吻合的方法可用于研究基因工程小鼠的血管疾病。它也可作为一种改良的动脉瘤模型结合移植。
在过程中有关键点。缝合线本身的放置是最关键的一步。外科医生必须小心, 以避免任何扭转的主动脉通过适当的协调捐助者和受体。在检查吻合口后, 要小心地取下夹具。远端钳位应始终首先释放导致低压保持墙壁在一起之前, 在近端高压侧释放。不正确地跟随释放序列的后果将是流血。

正如先前的一项研究指出的, 对小鼠主动脉移植模型给予了极大的关注, 这种模式允许在移植物本身引起的特定血管反应和与 arteriogenic 环境有关的某些系统因素之间进行区分。1,2,3. 在这里扮演关键角色的主要因素是淘汰赛和转基因小鼠的可用性。他们参与这种模式提供了确定和确定新的病理生理学途径的可能性与发展的退行性血管疾病, 如动脉粥样硬化和动脉瘤形成4,5。
值得注意的是, 在移植过程中, 血管内的缺血/再灌注损伤可能会出现。因此, 在术后期间发生的具体问题与移植完整性或意想不到的炎症反应不能排除可能解除退行性血管疾病的病理生理改变3 ,4,5,6,7。套管吻合是一种替代的 end-in-end 方法, 用于动脉吻合直径小于一毫米的血管, 并已成功应用于大鼠的肾和心脏移植, 随后适应主动脉小鼠移植的 Dambrin et al.8,9,10,11。
主动脉损伤使用袖状移植技术是最小的, 技术故障率非常低, 由于它只持续20分钟平均。我们以前的研究结果表明, 在移植使用套筒技术1后, isograft在体内具有优异的功能和结构特性。Dambrin et al.描述在短学习曲线之后, 成功率超过 78%10。并发症, 如血栓形成是罕见的, 例如 Engelbrecht et al.没有观察到血栓形成使用套筒技术在肾移植在大鼠8。
小鼠主动脉移植模型与套筒吻合是一种快速、简便的研究同种异体移植血管反应的工具。这段视频说明了在我们实验室进行的主动脉移植手术。这种移植模型可能有助于确定血管退行性疾病的潜在病理机制, 并可能有助于进一步评价分子和药理干预措施12。

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using the sleeve technique in a mouse model of aortic transplantation - an instructional video

采用袖套技术的原位主动脉移植可减少10-20% 的失败率, 降低主动脉的损伤。使用套筒法吻合小鼠主动脉的时间较短, 易于平均20分钟, 允许对同种异体移植进行研究。下面的文章介绍了主动脉移植术在我们的实验室中的应用。小鼠用1.5% 容积异氟醚和100% 氧气的混合物在面罩中麻醉。此时, 肾动脉与其分支之间的动脉段与腔静脉分离, 自由制备, clampedat 近端和远端段, 单丝缝合。在主动脉切除之前, 将含肝素的盐水溶液注入下腔静脉。然后在钳夹之间切断主动脉, 用生理盐水肝素溶液冲洗腔内。采用单丝缝合袖套技术, 在原位移植腹主动脉。

小鼠在15-30 分钟内从麻醉中恢复, 没有观察到身体损伤, 尽管血栓形成的风险很高。术后随访采用超声分析。在研究中使用的野生型小鼠, 其腔内的尺寸没有变化。因此, 没有观察到狭窄和动脉瘤形成。移植动物未能表现出血管壁的斑块发育 (图 2)。
常规染色方法如 immunohistology 可用于测定斑块、平滑肌细胞的形态和巨噬细胞的积累。在本研究中, 移植后6周进行组织学和免疫组化染色, 以检验移植物的完整性。组织学染色 (苏木精和曙红 (he) 和免疫组织化学染色 (平滑肌肌动蛋白 (SMA) 和巨噬细胞 (MAC2)) (图 3) 显示我们不变的平滑肌细胞分布模式, 完整的内皮细胞衬里, 没有细胞在内膜中的积累。这些结果表明, 移植血管中没有发现明显的病变或细胞活化 (图 3)。
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/54915/54915fig1.jpg"> 
图 1: 套筒技术.腹主动脉采用袖套法移植。在这个过程中, 捐献者的主动脉被放置在原位, 表面的叮咬存在于喂食容器中。<a href="//ecsource-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/54915/54915fig1large.jpg" target="_blank">请单击此处查看此图的较大版本.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/54915/54915fig2.jpg"> 
图 2: 术中和超声图像.例术中 (尸检) 观察移植术后6周 (A), 其三维超声 (B) 和 B 模式视图 (C)。术后随访采用超声进行。图片显示了移植的通畅性, 在流明尺寸上没有变化。此外, 没有观察到狭窄或动脉瘤形成。<a href="//ecsource-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/54915/54915fig2large.jpg" target="_blank">请单击此处查看此图的较大版本.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/54915/54915fig3.jpg"> 
图 3: 组织学和免疫组织化学图像.移植后6周移植动物的组织学和免疫组化代表图像。在移植脉中未观察到明显的病变 (苏木精和曙红, he, 100X 放大倍率, 刻度棒 = 50 µm), 免疫组织化学染色 (平滑肌肌动蛋白 SMA (红色), 或巨噬细胞 MAC2 (绿色), 200X 放大倍数, 刻度条 =25µm)。细胞核由 DAPI (蓝色) counter-stained。<a href="//ecsource-jove-com.remotexs.ntu.edu.sg/files/ftp_upload/54915/54915fig3large.jpg" target="_blank">请单击此处查看此图的较大版本.</a>

Watch this Scientific Journal Video about Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video at JoVE.com

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

本文采用套筒技术对小鼠原位主动脉移植模型进行了研究。它是一种非常快速的吻合方法, 可用于血管疾病的研究。

套筒技术在主动脉移植小鼠模型中的应用-教学视频

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

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JoVE Journal

Medicine

8.9K 次观看.  Ruhr- University Bochum. 本文采用套筒技术对小鼠原位主动脉移植模型进行了研究。它是一种非常快速的吻合方法, 可用于血管疾病的研究。

视频: Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. This is largely true, but there are a number of important principles that can reduce the difficulty of these procedures and enhance reproducibility. Orthotopic aortic transplantation is an excellent procedure in which to learn these principles because it involves only two end-to-end anastomoses, but requires good suturing technique and handling of the vessels for consistent success. This procedure begins with the procurement of a length of abdominal aorta from a donor animal, followed by division of the native aorta in the recipient. The procured aorta is then placed between the divided ends of the recipient aorta and sutured into place using end-to-end anastomoses. To accomplish this objective successfully requires a high degree of concentration, good tools, a steady hand, and an appreciation of how easily the vasculature of a mouse can be damaged, resulting in thrombosis. Learning these important principles is what occupies most of the beginner's time when learning microsurgery in small rodents. Throughout this protocol, we refer to these important points. This model can be used to study vascular disease in a variety of different experimental systems1-8. In the context shown here, it is most often used for the study of post-transplant vascular disease, a common long-term complication of solid organ transplantation in which intimal hyperplasia occurs within the allograft. The primary advantage of the model is that it facilitates quantitative morphometric analyses and the transplanted vessel lies contiguous to the endogenous vessel, which can serve as an additional control9. The technique shown here is most often used for mice weighing 18-25 grams. We have accumulated most of our experience using the C57BL/6J, BALB/cJ, and C3H/HeJ strains.

We describe a technique in which a section of the abdominal aorta from a mouse is transplanted orthotopically to just below the renal arteries in an allogeneic or syngeneic recipient. This technique can be useful in studies in which transplantation of large arteries of uniform size is deemed advantageous.

原位主动脉瓣移植在小鼠血管疾病的研究

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

科研

医学

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are available to perform mechanistic in vivo studies. While heart transplantation models using a suture technique were first successfully developed in rats, the translation into an equally widespread used murine equivalent was never achieved due the technical complexity of the microsurgical procedure. In contrast, non-suture cuff techniques, also developed initially in rats, were successfully adapted for use in mice1-3. This technique for revascularization involves two major steps I) everting the recipient vessel over a polyethylene cuff; II) pulling the donor vessel over the formerly everted recipient vessel and holding it in place with a circumferential tie. This ensures a continuity of the endothelial layer, short operating time and very high patency rates4.
Using this technique for vascular anastomosis we performed more than 1,000 cervical heart transplants with an overall success rate of 95%. For arterial inflow the common carotid artery and the proximal aortic arch were anastomosed resulting in a retrograde perfusion of the transplanted heart. For venous drainage the pulmonary artery of the graft was anastomosed with the external jugular vein of the recipient5.
Herein, we provide additional details of this technique to supplement the video.

The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach.
Herein, we provide additional details of this technique to supplement the video.

用改进的袖套法小鼠颈部心脏移植模型

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

Murine Heterotopic Heart Transplant Technique

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become proficient in and utilize this technique, it is now widely used by many laboratories around the world. A significant refinement to the original technique was developed and reported in 2001 by Niimi. Described here are the techniques that have evolved over more than a decade in the hands of three surgeons (Plenter, Grazia, Pietra) in our center. These techniques are now being passed on to a younger generation of surgeons and researchers.
Based largely on the Niimi experience, the procedures used have evolved in the fine details - details which we will endeavor to relate here in such a way that others may be able to use this very useful model. Like Niimi, we have found that a video aid to learning is a priceless resource for the beginner.

The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.

小鼠异位心脏移植技术

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become ...

Optimization of the Cuff Technique for Murine Heart Transplantation

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to improve surgical efficiency. In our lab, we have optimized the cuff technique with two major steps. First, we used the inner tube technique to insert a temporary inner tube into the external jugular vein and carotid artery blood vessel to facilitate eversion of the vessel over the cuff. Second, we performed complete heterotopic cardiac transplantation through the collaboration of two experienced surgeons. These modifications effectively reduced the operation time to 25 minutes, with a success rate of 95%. In this report, we describe these procedures in detail and provide a supplemental video. We believe that this report on the improved cuff technique will offer practical guidance for murine heterotopic heart transplantation and will enhance the utility of this mouse model for basic research.

We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

穆林心脏移植袖口技术的优化

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to ...

免疫与感染

Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

Murine models of cardiac transplantation are frequently utilized to study ischemia-reperfusion injury, innate and adaptive immune responses after transplantation, and the impact of immunomodulatory therapies on graft rejection. Heterotopic cervical heart transplantation in mice was first described in 1991 using sutured anastomoses and subsequently modified to include cuff techniques. This modification allowed for improved success rates, and since then, there have been multiple reports that have proposed further technical improvements. However, translation into more widespread utilization remains limited due to the technical difficulty associated with graft anastomoses, which requires precision to achieve adequate length and caliber of the cuffs to avoid vascular anastomotic twisting or excessive tension, which can result in damage to the graft. The present protocol describes a modified technique for performing heterotopic cervical cardiac transplantation in mice which involves cuff placement on the recipient's common carotid artery and the donor's pulmonary artery in alignment with the direction of the blood flow.

Mouse cardiac transplantation models represent valuable research tools for studying transplantation immunology. The present protocol details mouse heterotopic cervical cardiac transplantation that involves the placement of cuffs on the recipient's common carotid artery and the donor's pulmonary artery trunk to allow for laminar blood flow.

利用血管袖带的小鼠异位宫颈心脏移植

Murine models of cardiac transplantation are frequently utilized to study ischemia-reperfusion injury, innate and adaptive immune responses after ...

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. However, only minor improvement in the long-term results of transplanted solid organs could be observed over the past decades. In this context, chronic allograft vasculopathy (CAV) still represents the leading cause of late organ failure in cardiac, renal and pulmonary transplantation.
Thus far, the underlying pathogenesis of CAV development remains unclear, explaining why effective treatment strategies are presently missing and emphasizing a need for relevant experimental models in order to study the underlying pathophysiology leading to CAV formation. The following protocol describes a murine heterotopic cervical aortic transplantation model using a modified non-suture cuff technique. In this technique, a segment of the thoracic aorta is interpositioned in the right common carotid artery. With the use of the non-suture cuff technique, an easy to learn and reproducible model can be established, minimizing the possible heterogeneity of sutured vascular micro anastomoses.

Here, we present a protocol of heterotopic aortic transplantation in mice using the non-suture cuff technique in a cervical murine model. This model can be used to study the underlying pathology of chronic allograft vasculopathy (CAV) and can help evaluate new therapeutic agents in order to prevent its formation.

使用改性非缝合铐技术的宫颈主动脉移植模型

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. ...

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Cardiac allograft rejection limits the long-term survival of patients after heart transplantation. A mouse heart transplantation model is ideal for investigating the mechanism of cardiac allograft rejection in preclinical studies because of their high homology with human genes. This understanding would help develop unique approaches to improving patients&#39; long-term survival treated with cardiac allografts. In a mouse model, abdominal donor heart implantation is commonly performed with an end-to-end&#160;anastomosis to the recipient&#39;s aorta and inferior vena cava using stitches. In this model, the donor&#39;s heart is implanted by end-to-end anastomosis to the recipient&#39;s carotid artery and jugular vein by the modified-Cuff technique. The transplantation surgery is performed without stitching and thus may increase the survival of the recipient since there is no interference with the blood supply and venous reflux of the lower body. This mouse model would help investigate the mechanisms underlying the immunological and pathological (acute/chronic) rejection of cardiac allografts.

In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.

一种改进的袖带技术用于小鼠颈椎异位心脏移植模型

Cardiac allograft rejection limits the long-term survival of patients after heart transplantation. A mouse heart transplantation model is ideal for ...

小鼠肝移植中有效血管吻合的策略

Vascular Reconstruction with the Cuff Technique in Mouse Orthotopic Liver Transplantation

Mouse orthotopic liver transplantation is an effective methodology for investigating the underlying mechanisms of liver ischemia and reperfusion injury. However, the technical challenges pose a barrier to utilizing this valuable experimental model and passing on these skills to the next generation. The most challenging aspect of this procedure is vascular reconstruction, including the portal vein (PV), infrahepatic inferior vena cava (IHIVC), and suprahepatic inferior vena cava. The use of plastic cuffs, rather than sutures, allows for smoother PV and IHIVC reconstruction. Vessels are reconstructed by attaching a cuff made from an intravenous catheter to the tip of the graft vessel and interposing the cuff into the recipient vessel. The two most crucial aspects are properly visualizing the inner lumen of the vessel and avoiding the use of excessive force. Our aim is to provide a technical overview of vascular reconstructions using the cuff technique in recipient surgery. These technical tips for the cuff technique are expected to help microsurgeons facilitate vascular reconstruction and advance their research.

作者聚焦：探索脂肪肝移植中的冷缺血和温再灌注损伤

This protocol provides technical information for vessel reconstruction using the cuff technique in mouse orthotopic liver transplantation.

袖带技术在小鼠原位肝移植中的血管重建

Mouse orthotopic liver transplantation is an effective methodology for investigating the underlying mechanisms of liver ischemia and reperfusion injury. ...

套筒技术在主动脉移植小鼠模型中的应用-教学视频

摘要

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此视频中的章节

原位主动脉瓣移植在小鼠血管疾病的研究

用改进的袖套法小鼠颈部心脏移植模型

小鼠异位心脏移植技术

穆林心脏移植袖口技术的优化

利用血管袖带的小鼠异位宫颈心脏移植

使用改性非缝合铐技术的宫颈主动脉移植模型

一种改进的袖带技术用于小鼠颈椎异位心脏移植模型

袖带技术在小鼠原位肝移植中的血管重建

袖带技术在小鼠原位肝移植中的血管重建

袖带技术在小鼠原位肝移植中的血管重建