作者想感谢Ted的Van de Beek的波士顿科学公司在编制这份手稿对他的支持。

1。行前准备程序<ol><li>在执行过程之前，讨论的风险和利益的程序与病人，并获取签名的同意书。调整病人的维生素K拮抗剂剂量，以达到国际标准化比率为2.0〜3.0。继续抗心律失常药物治疗的程序的时间。 </li><li>无论是前一天或一​​天​​的程序，执行经食管超声心动图（TEE）来记录的情况下在LAA内血栓，评估在LAA的特性和类型，以确定适当的彪形大汉设备大小植入（见2012年朱庇特的更多信息，文章利用Mobius温克勒和他的同事）22。 </li><li>执行合并的导管射频消融和WATCHMAN左心耳闭合装置放置过程中的电生理实验室适当的诊断和成像设备的过程中。 </li><li>随着病人取仰卧位，administeŕ麻醉根据机构的协议。 </li></ol> 2。射频消融治疗<ol><li>确保电生理记录系统（巴德公司，马萨诸塞Lowell）正在使用的过滤器设置为100〜500 kHz和一个信号放大为5,000。 </li><li>一旦患者完全麻醉，管理20毫升1％利多卡因局部麻醉在腹股沟区。随即，清洁和准备腹股沟区插管。 </li><li>找到股静脉，插入静脉法国（8F）护套。介绍四极起搏导管插入冠状静脉窦。然后，将一个很长的导丝，取出护套。介绍一个标准的的静脉跨室间隔访问鞘。 </li><li>长的导丝取出，然后插入跨室间隔RF Brockenbrough针（贝里斯医疗器材公司，蒙特利尔，魁北克，加拿大），一个12.5F-outer-diameter/9.5F-inner-diameter可控的护套（通道，吟游诗人，洛厄尔，MA ），进行跨室间隔标点符号重。 </li><li>穿过房间隔后，猪尾巴导管在左心房的中心定位，并进行3D旋转血管造影。 </li><li>肺静脉消融导管（PVAC:美敦力/射频消融前沿，公司，卡尔斯巴德，加利福尼亚州）的是一个9F，过线，圆形，decapolar的映射和一个25毫米直径的阵列在远端冰山的消融导管。的的PVAC有10个电极在镍钛记忆合金框架，使肺静脉隔离的映射，消融和验证的。 0.032英寸的导丝插入到PVAC。 </li><li>下一步，将整个系统进入静脉鞘。由聚醋酸乙烯酯中收集到的信息被显示在监视器上，以产生与病人的心脏的三维图像显示的导管的位置。 </li><li>提供一个作为该过程的结果，以防止凝血丸10.000IE肝素。定位线的肺静脉的分支之一，然后提前进入肺静脉前庭的聚醋酸乙烯酯。 </li><li>使用fluoroscopiÇ指导，心电图和3D的电信号成像（ESI）重建评估的聚醋酸乙烯酯的位置进行调整，以获得最佳的接触与所有的电极。 </li><li>一旦达到一个令人满意的导管的位置，展开，通过选择适当的电极对提供的射频能量的射频（RF）消融。 </li><li>设置发电机调整功率来实现的目标温度为60℃，在所选择的对各电极。在大多数情况下，电极对将被激活，在第一个应用程序创建的重叠性病灶环。 </li><li>消融为60秒。检查显示器在消融过程中的条件。在15秒内，如果温度不高于50°C，终止应用程序，调整电极的位置，并重新开始。 </li><li>移动和旋转PVAC-导管环肺静脉病变。 </li><li>所有肺静脉重复此过程。 </li><li> CR后吃圆周所有肺静脉的病变，恢复窦性节律以直流电流的律（DCCV）。 </li><li>冠状静脉窦起搏演习结合各静脉内聚醋酸乙烯酯映射的局部电位（入口和出口块）的情况下检查，确认隔离。 </li><li>对于独立的电极对上残留的潜力，目标消融和相邻的一对，有利于达到目标温度。 </li></ol> 3。植入的彪形大汉设备<ol><li>一旦导管消融治疗的程序是完整的，守望的装置植入。 </li><li>推进0.035"硬导丝（ 如消融超级僵硬260厘米）成聚醋酸乙烯酯和位置的导丝在左上肺静脉。聚醋酸乙烯酯，同时保持导丝的位置。 </li><li>如果消融不进行植入前，肝素100 IU / kg体重管理得到活化凝血时间of 200-300秒。检查活化的凝血时间的水平，以确保，它仍然是200-250秒。根据需要继续监控，每30分钟。 </li><li>删除守望的包装在无菌条件下接入系统和扩张。仔细检查是否有损坏。 </li><li>填充60CC注射器用生理盐水冲洗的侧端口的彪形大汉访问的套。使用剩余的生理盐水冲洗扩张。将扩张的彪形大汉访问护套。 </li><li>推进彪形大汉接入鞘和扩张器在导丝进入左心房。作为守望的访问的护套接近中心的左心房，保持扩张和推进的初始位置，只在左心房的的彪形大汉访问鞘。 </li><li>取出扩张器和导线。在拧紧止血阀，允许回流血的可能性降到最低空气引入。用生理盐水冲洗彪形大汉访问护套。 </li><li>拧紧阀门的访问鞘。 </li><li>组（CON）公司LAA的尺寸，使用TEE测量的最大的LAA尺寸在4次，在0°，45°，90°和135°。 </li><li>用生理盐水冲洗猪尾巴导管在守望的访问护套线，并通过推进。然后，取出导丝连接的注射器造影剂的小辫子。 </li><li>在查看TEE，把守望的访问鞘逆时针调整鞘前推进的小辫子在所需的位置在远端部分的LAA。 </li><li>一旦猪尾巴导管已经达到了LAA，获得血管造影在右前斜（RAO）20〜30℃，尾鳍20-30℃。然后执行TEE用最少的0-135°扫描。套先进的LAA附近的墙或顶点时，同时促进更多的远侧任何解剖，这是最重要的。 </li><li>小心地推进访问最优越的叶猪尾导管鞘管，在血管造影，这是LAA叶situat2点左右在一个右前斜（RAO）20〜30℃，尾椎20〜30°，，，TEE它是最右边的叶LAA-135°。 </li><li>透视引导下，提前的彪形大汉访问鞘中的所需深度的基础上的标志带，对应到合适的大小LAA。 </li><li>一旦尽可能的小辫子是远端，注入造影剂。 </li><li>选择适当的彪形大汉器件尺寸的基础上最大LAA口尺寸和浆纱22。 </li><li>在无菌条件下，取出的的彪形大汉设备从包装，检查是否有损坏。要确认的设备连接到芯线，打开止血阀和缩回的移动设备大约1厘米。 </li><li>的移动设备的标记带的远侧尖端对齐。守望的设备不应突出。 </li><li>将一个大的含盐水60毫升注射器。系统冲洗几次，以除去任何空气。然后，淹没在输送导管的前端盐水拍打，以除去气泡。 </li><li>重新检查血管造影的访问鞘的位置。松开接入止回阀，从鞘中，慢慢取出的猪尾巴导管。允许套回流血。 </li><li>为了避免引入空气，通过注入生理盐水冲洗口淌从输送导管，然后，引进的彪形大汉设备系统。 </li><li>透视引导下，慢慢地推进输送导管进入接入鞘接入鞘线，直至最前端的标记带的标记带输送导管和稳定输送导管。 </li><li>收回的访问鞘和捕捉它的输送导管上。使用透视，再次确认在输送导管尖端的位置。不提前一次访问鞘输送系统已被抢购一空。 </li><li>如果需要重新定位，将输送系统，重新插入猪尾巴导管和推进的访问鞘为PROPER位置。 </li><li>一旦输送系统的位置，松开彪形大汉输送导管上的阀门。观察远端设备，以确保没有向前推进，或重新定位相对口。 </li><li>然后，向设备部署，持有部署旋钮静止和慢慢地缩回接入鞘/输送导管组件用一个缓慢超过3-5秒的期间的稳定的运动。 </li><li>撤回访问护套/输送导管组件，从设备到几厘米将它与LAA，留下的芯线连接。 </li><li>一旦设备已经部署，再次注入对比。然后，采用X光透视TEE，确认位置，锚，尺寸和密封（PASS）设备发布标准得到满足如下: </li><li> 的位置是 :确认该设备被正确地定位，确保该装置的最大直径的平面跨越在LAA口和等于或恰好远侧孔口在LAA。 </li> &lt;李&gt; 主持人:确认设备是固定到位，，通过撤回的访问护套/交付导管组件1 - 2厘米面对的设备，然后轻轻地收回，并释放部署旋钮。 LAA和设备异口同声地移动。 </li><li> 大小:确认该设备是通过测量该装置的最大直径的平面在0°，45°，90°，135°，确保带螺纹的插入件是可见的标准4次使用TEE适当尺寸。该装置应该是80-92％的原始大小。 </li><li> 密封:采用彩色超声多普勒，确保所有的远端设备的叶是密封的。彩色流不应检测装置附近。如果有一个设备周围的是大于5毫米的间隙或喷射，该装置应被重新定位或完全夺回和替换。 </li><li>如果所有的发行条件得到满足，移动接入鞘/输送导管在脸上的设备和旋转部署逆时针转动旋钮3-5释放。 </li><li>发行完成后，造影剂进行血管造影记录，该装置仍处于地方。然后，使用TEE，请重新检查尺寸和密封。删除套管组件的左心房。 </li><li>如果该设备是在LAA或设备不符合标准的远端或近端，它可能是必要的部分或全部夺回设备（有关详细信息，请参阅莫比乌斯温克勒和他的同事们在2012年的朱庇特的文章）22。 </li></ol> 4。后程序<ol><li>一旦妥善安置的设备已被证实，取出护套。股静脉，然后被压缩了几个小时，至少6个小时的血压和心脏率监测病人应进行仔细的监测。 </li><li>请血肿和/或出血，定期根据机构的指导方针。每次的患者应给予抗生素预防Ë美国心脏协会的指导方针。注意:POST程序肝素不推荐使用。 </li><li>一旦病人是清醒的，进行神经系统检查。 </li><li>患者应保持至少45天的华法林和阿司匹林81毫克后的的植入程序（内部标准化比值= 2.0-3.0）。 </li><li>病人应住院过夜，翌日康复出院。经胸超声心动图（TTE）或胸部X射线可以进行确认的情况下，心包积液。 </li><li>在45天的评估守望的设备放置TEE。停止华法林是在医生的自由裁量权，如果完全封闭的LAA血栓的设备上被排除。如果流注意到设备周围的大于5毫米，应考虑使病人华法林，直到它已经降低到小于5毫米。 </li><li>停止华法林的患者应开始每天75毫克氯吡格雷经过6个月后植入物，并继续采取阿司匹林每天下去。 </li><li>植入后6个月内订定适当的性心内膜炎的预防。超过6个月的持续性心内膜炎的预防，是在医生的自由裁量权。 </li></ol>

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R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Left+atrial+appendage+closure.">Left atrial appendage closure.</a> Current Cardiology Reports. 12, 413-421 (2010).</li><li>Velthuis, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Left+Atrial+Appendage+Closure+for+Stroke+Prevention+in+Patients+with+Atrial+Fibrillation+and+Hereditary+Hemorrhagic+Telangiectasia.">Left Atrial Appendage Closure for Stroke Prevention in Patients with Atrial Fibrillation and Hereditary Hemorrhagic Telangiectasia.</a> Case Reports in Cardiology. 3, (2012).</li><li>Swaans, M. J., Post, M. C., Rensing, B. J., Boersma, L. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Percutaneous+left+atrial+appendage+closure+for+stroke+prevention+in+atrial+fibrillation.">Percutaneous left atrial appendage closure for stroke prevention in atrial fibrillation.</a> Netherlands Heart Journal: Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation. 20, 161-166 (2012).</li><li>Mobius-Winkler, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+WATCHMAN+left+atrial+appendage+closure+device+for+atrial+fibrillation.">The WATCHMAN left atrial appendage closure device for atrial fibrillation.</a> J. Vis. Exp. (60), e3671 (2012).</li><li>Garcia-Fernandez, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+left+atrial+appendage+obliteration+in+stroke+reduction+in+patients+with+mitral+valve+prosthesis:+a+transesophageal+echocardiographic+study.">Role of left atrial appendage obliteration in stroke reduction in patients with mitral valve prosthesis: a transesophageal echocardiographic study.</a> Journal of the American College of Cardiology. 42, 1253-1258 (2003).</li><li>Blackshear, J. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thoracoscopic+extracardiac+obliteration+of+the+left+atrial+appendage+for+stroke+risk+reduction+in+atrial+fibrillation.">Thoracoscopic extracardiac obliteration of the left atrial appendage for stroke risk reduction in atrial fibrillation.</a> Journal of the American College of Cardiology. 42, 1249-1252 (2003).</li><li>Block, P. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Watching+the+watchman.">Watching the watchman.</a> Journal of the American College of Cardiology. 49, 1496-1497 (2007).</li><li>Block, P. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=+Percutaneous+left+atrial+appendage+occlusion+for+patients+in+atrial+fibrillation+suboptimal+for+warfarin+therapy:+5-year+results+of+the+PLAATO+(Percutaneous+Left+Atrial+Appendage+Transcatheter+Occlusion)+Study."> Percutaneous left atrial appendage occlusion for patients in atrial fibrillation suboptimal for warfarin therapy: 5-year results of the PLAATO (Percutaneous Left Atrial Appendage Transcatheter Occlusion) Study.</a> JACC. Cardiovascular. 2, 594-600 (2009).</li><li>Viles-Gonzalez, J. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Incomplete+occlusion+of+the+left+atrial+appendage+with+the+percutaneous+left+atrial+appendage+transcatheter+occlusion+device+is+not+associated+with+increased+risk+of+stroke.">Incomplete occlusion of the left atrial appendage with the percutaneous left atrial appendage transcatheter occlusion device is not associated with increased risk of stroke.</a> Journal of Interventional Cardiac Electrophysiology: An International Journal of Arrhythmias and Pacing. 33, 69-75 (2012).</li><li>Cappato, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Worldwide+survey+on+the+methods,+efficacy,+and+safety+of+catheter+ablation+for+human+atrial+fibrillation.">Worldwide survey on the methods, efficacy, and safety of catheter ablation for human atrial fibrillation.</a> Circulation. 111, 1100-1105 (2005).</li><li>Reddy, V. Y., Holmes, D., Doshi, S. K., Neuzil, P., Kar, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Safety+of+percutaneous+left+atrial+appendage+closure:+results+from+the+Watchman+Left+Atrial+Appendage+System+for+Embolic+Protection+in+Patients+with+AF+(PROTECT+AF)+clinical+trial+and+the+Continued+Access+Registry.">Safety of percutaneous left atrial appendage closure: results from the Watchman Left Atrial Appendage System for Embolic Protection in Patients with AF (PROTECT AF) clinical trial and the Continued Access Registry.</a> Circulation. 123, 417-424 (2011).</li><li>Oral, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+vein+isolation+for+paroxysmal+and+persistent+atrial+fibrillation.">Pulmonary vein isolation for paroxysmal and persistent atrial fibrillation.</a> Circulation. 105, 1077-1081 (2002).</li><li>Biase, L. D. i., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Left+atrial+appendage:+an+underrecognized+trigger+site+of+atrial+fibrillation.">Left atrial appendage: an underrecognized trigger site of atrial fibrillation.</a> Circulation. 122, 109-118 (2010).</li></ol>

心内科圣安医院（荷兰Nieuwegein）监考费，培训/教育服务从Atritech /波士顿科学。 生产和免费使用这篇文章是由波士顿科学。

上面的文字描述作为房颤治疗和预防相关的血栓栓塞事件的联合导管消融和左心耳闭塞的程序。初步数据显示，这个过程可以安全地进行，患者在中风的高风险。执行合并过程中遇到的技术困难。虽然没有任一过程的改善，因为它们不互相影响，可以预期，合并的程序分别执行的程序，可以提供几个优点。合并过程中，可同时治疗房颤的症状和中风的风险减少，改善生活质量，为那些受AF。它也可能出现药物难治性患者的治疗方案，让他们接受植入的彪形大汉设备没有后续的华法林治疗。此外，反复的必要性，以及相关的风险，左心房减少干预和跨室间隔穿刺左心耳封闭成为理想的随访期间。 CA是一个既定的AF症状治疗，而最近的数据表明，它也是有效降低中风。在匹配的AF患者对回顾性分析，雷诺和他的同事们看到了一个显着减少卒中和短暂性脑缺血发作的患者接受消融相比，那些接受抗心律失常药物治疗16事件的相对危险性。数据还表明，通过手术结扎或设备放置有效地降低血栓事件截肢的LAA或闭塞。这些方法似乎不错:二尖瓣置换术的患者在一项研究中，接受手术结扎显著较少的招（3％和17％），19，23。一种微创，胸腔镜结扎使用循环网罗程序已成功执行14 15患者中风发生2例（4％）以上的下列42个月19,24。 的几个经皮导管LAA闭塞装置已被开发，包括应用Amplatzer心脏插头（AGA医疗），经导管修补程序（自定义医疗器械），经皮LAA的经导管的闭塞（PLAATO）系统，和左心耳封堵器的彪形大汉。目前，有关安全性和有效性的临床研究才公布PLAATO（这是不再可用）的彪形大汉。 PLAATO证明安全性和有效性，并提供证明LAA封闭使用镍钛记忆合金为基础的过滤器设备的概念。该器件是自膨式镍钛合金笼覆盖聚四氟乙烯是阻止血液从进入LAA 25。 64例的一个CHADS 2&gt; 2的非随机研究表明超过98％的患者完全闭塞。 5年后，卒中/短暂性脑缺血阿塔克施钾量减少至3.8％，较预期的6.6％，人口26。 从保护AF 18日 ，与华法林治疗的疗效比较的彪形大汉设备的研究获得的数据进一步支持基于设备的方法LAA闭塞。这项随机研究显示，植入彪形大汉被认为是导致中风和各种原因的死亡率在OAC符合条件的患者在非劣效性，提高的可能性，该过程最终可以被用来作为一种替代华法林治疗慢性患者谁是药物折射18。在荷兰心脏杂志上发表的研究，在2012年21，我们成功植入10例，9例并发CA的设备。需要注意的是，虽然剩余流量在45天随访有3例，Viles - 冈萨雷斯和他的同事证明，这个设备周围的剩余流量是不相关的一个增加的血栓栓塞事件风险27。 虽然现有的数据支持CA和使用的的彪形大汉设备作为有效的治疗AF的LAA闭塞，这两个过程都伴有并发症。一个世界性的加利福尼亚州进行的一项调查显示，在2010年的并发症发生在4.5％的患者14。最显着的并发症包括:死亡，脑血管血栓栓塞事件，extrapericardial肺静脉穿孔，心包填塞，狭窄，新发（医源性）14，15，28和非典型扑。从彪形大汉设备安置程序，可能会导致手术并发症包括严重的心包积液，设备栓塞术，手术相关的行程18。并发症的发生率在这两种情况下，已被发现依赖于操作者的经验15，29。 对于合并的过程中，安全方面的数据是有限的，我们的2012年的研究，在其中的COM褶皱被限制在一台设备等症。一半的患者并没有在3个月的随访记录房颤复发的。另一半的患者需要至少一个直流电复律。 1例患者重复消融，它是成功的21。虽然本研究是一小口，并且不提供直接的比较，与其他的治疗方法，在每一个独立的程序29中的安全性的研究，30表明，治疗抵消了安全风险增加的潜在好处。额外的安全性要考虑的问题是程序的时间，全身麻醉的要求，并增加胃肠道出血的风险与TEE监测，必须伴随着彪形​​大汉放置的时间越长。 虽然本文描述的方法描述了一种组合的程序彪形大汉放置和PV CA，重要的是要注意，彪形大汉放置不排除一方向分析y的LAA的电气隔离。迪BIASE和他的同事们最近的一项研究表明，在选定的组房颤复发的患者中，27％表明灶LAA后所产生的初始消融，这是最佳的处理通过完整的圆周LAA隔离31。这组病人特别是受益于LAA关闭，因为电气隔离的LAA可能导致LAA淤滞，甚至在窦性节律，使之更血栓形成。被认为是房颤消融和左心耳封闭的患者，可先进行电生理与药理挑战进行检测和消融可能LAA之前触发植入的彪形大汉设备31。 由于本研究是随机的，被限制在45天的随访10个高风险的病人，许多问题仍有待解决:合并CA /彪形大汉植入过程中更有效地行程公关evention和救灾的AF症状，仅比任何一种方法？利益的程序如何比较，如果程序的顺序是相反的，与闭塞第一LAA？如何安全的程序相比，无论是设备植入或CA吗？这是在低风险的患者有效？是需要的未来合并过程受消融？的程序进行在不服用华法林的患者中同样获得成功吗？未来的研究将包括一项随机对照研究，以研究是否将这些技术可以提高患者的预后和生活质量，在这些患者中。 请注意，执行此操作时，重要的是要仔细评估LAA解剖时，器件的尺寸和选择的设备放置在发布前进行全面检查。这也是非常重要的，以确保设备的降落区域的深度为等于或大于的最大宽度的第ËLAA口。这些步骤是为病人的安全性和有效性的程序的关键。

catheter ablation in combination with left atrial appendage closure for atrial fibrillation

心房纤维性颤动（AF）是最常见的持续性心律失常，影响到数百万世界各地的个人1-3。快速，不规则，无序的心房电活动会引起心悸，乏力，呼吸困难，胸痛，头晕或不晕厥4，5。 AF的患者有5倍的风险较高，行程6。 常用的口服抗凝药物（OAC）与华法林的房颤患者中风的预防和已被证明是中风的风险降低了64％7。华法林治疗有几大缺点，但是，包括出血，不宽容，与其他药物和食品的相互作用，不符合规定和狭窄的治疗范围8-11。这些问题，连同升值的风险 - 效益比差，不注意的指引，或没有OAC监控门诊诊所也许可以解释为什么只有30-60％的患者与AF处方这种药物8。 与华法林的疗效有限和/或严重的副作用，与其他药物用于AF 12,13，结合相关的问题，强调需要有效的非药物治疗方法。一种方法是导管消融（CA），一个射频电流应用到心脏地区的一个过程，在这过程中创建小的消融灶，电隔离潜在的AF触发4。 CA是一个行之有效的治疗AF症状，14，15，也可以减少中风的危险。最近的数据显示，显着降低卒中和短暂性脑缺血发作的患者接受消融相比，那些接受抗心律失常药物治疗16事件的相对危险性。 由于左心耳（LAA），在超过90％的患者中是血栓源患者与非瓣膜性房颤17，另一种方法是用身体挡住退出LAA血栓预防中风。封闭LAA的方法之一，是通过经皮放置的WATCHMAN左心耳封堵器。守望的设备，类似于一个小型降落伞。它由覆盖镍钛合金帧，由织物聚对苯二甲酸乙二醇酯，以防止栓子，但没有血液，在愈合过程中排出。周围的周边的固定锚固定在LAA的（ 图1）的移动设备。至目前为止，守望的是唯一的植入经皮设备上的随机临床试验已经报告。在这项研究中，植入的彪形大汉被认为是至少一样有效华法林在预防中风（原因）和死亡（所有原因）18。此设备获得了欧洲通用 （CE）标记的使用华法林在欧盟符合条件的患者和那些有一个反面的抗凝治疗19 traindication到。 由于行之有效CA，以减轻房颤的症状和有前途的数据，两个程序结合的，希望进一步降低中风的发病率在高风险的患者缓解症状的同时减少血栓栓塞事件与CA和WATCHMAN植入。合并后的过程可能会最终使患者接受植入的彪形大汉设备没有后续的华法林治疗，因为CA过程本身降低血栓栓塞事件。这将带来以前无法治疗的患者，不适合华法林治疗，因为经常出血或其他华法林相关问题的途径。 合并过程双翼飞机透视TEE指导下进行全身麻醉。通过植入的彪形大汉LAA closu的导管射频消融后重新装置。数据库中的10例患者的非随机试验表明，此过程中，可以安全地执行与CHADS2评分大于1 21患者。进一步研究，以研究在减少症状从AF和相关的中风的合并过程的有效性，因此必要的。

In a preliminary study of WATCHMAN implantation in 10 patients that had a high risk of stroke and bleeding, 9 of the patients received the device after a catheter ablation. The data from this study is published in the Netherlands Heart Journal, and is presented here with permission 21. All patients included in the study were fully informed about the procedure and signed a consent form. The study was approved by the hospital's ethics committee and all procedures were performed in accordance with the hospital's ethics standards and the Helsinki Declaration of 1975 (revised in 2008).
The characteristics of these patients are summarized in Table 1. All patients had documented AF with a high stroke risk (CHADS2 &gt;1) and all but one of the patients had history of stroke. LAA closure was indicated as an alternative to OAC for the following reasons: stroke during OAC therapy (3/10), severe bleeding during OAC therapy (2/10) and patient preference (5/10). Prior to the procedure all patients were taking a vitamin K antagonist. The median CHADS2 score for this group was 3, with a median CHA2DS2-VASc score of 3.5 and a median HAS-BLED score of 1.5. Prior to the procedure, all patients underwent TEE to exclude LAA thrombus and to rule out structural cardiac abnormalities. Among patients, the average maximum LAA diameter was 20.2&plusmn;2.2 mm with an average depth of 27.9&plusmn;5.4 mm. 40% of the patients had a multi-lobular atrial appendage.
Table 2 outlines procedural characteristics. The WATCHMAN LAA closure device was successfully placed in all 10 patients. Based on patient anatomy determined during preliminary TEE, three different device sizes were used. The median total procedure time was 104 min (range 75-167 min), including a median 56 min (range 38-137 min) for LAA occlusion. During the procedure, an asymptomatic catheter thrombus occurred in one patient. The thrombus was aspirated and the patient received an extra bolus of heparin. Prolonged femoral vein bleeding in this patient necessitated a blood transfusion and extended hospitalization. All other patients were discharged from the hospital on the day following the procedure (Table 2). OAC was continued at least until TEE was performed at 45 days, at which point the device position and closure rate was assessed.
The findings at the 45-day patient follow-up are summarized Table 3. Successful device implantation was defined as long-term sealing of the LAA as measured by TEE 45 days after the procedure without major adverse events. Residual flow (&lt; 5 mm) around the device was allowed after the procedure (according to the PROTECT-AF study). Importantly, no thromboembolic cerebral events occurred. Minimal residual flow was observed in 3 patients. TEE revealed asymptomatic embolization of the device in one patient, and the device was percutaneously retrieved via the femoral vein. Thrombus was not seen on the device in any cases. One patient required a redo-ablation procedure 3 months after the initial procedure, in which pulmonary vein isolation was successful. The implanted LAA device was not affected by and did not interfere with the procedure. Three of the 10 patients were able to discontinue warfarin treatment at 45 days. In the PROTECT-AF18 and Continues Access Registry29 device embolization occurred in 3 patients out of the 1,002 patients implanted.
Figure 2 shows a TEE screen capture of the LAA in one example patient in which no residual flow is observed 45 days post-procedure. In contrast, flow around the device, as evidenced by color Doppler, is seen for another patient (Figure 3).
<table border="1">
	<tbody>
		<tr>
			<td colspan="2">Baseline Characteristics</td>
		</tr>
		<tr>
			<td>Number, n</td>
			<td>10</td>
		</tr>
		<tr>
			<td>Age (years)</td>
			<td>61.6 +/9.6</td>
		</tr>
		<tr>
			<td>Sex (%female)</td>
			<td>5 (50)</td>
		</tr>
		<tr>
			<td>Median CHADS2</td>
			<td>3 (2-4)</td>
		</tr>
		<tr>
			<td>2</td>
			<td>4 (40)</td>
		</tr>
		<tr>
			<td>3</td>
			<td>4 (40)</td>
		</tr>
		<tr>
			<td>4</td>
			<td>2 (20)</td>
		</tr>
		<tr>
			<td>CHA2DS2-VASc</td>
			<td>3.5 (2-6)</td>
		</tr>
		<tr>
			<td>HAS-BLED</td>
			<td>1.5 (1-4)</td>
		</tr>
		<tr>
			<td>Vitamin K antagonist, n(%)</td>
			<td>10 (100)</td>
		</tr>
		<tr>
			<td colspan="2">Indication, n(%)</td>
		</tr>
		<tr>
			<td>Bleeding with OAC</td>
			<td>2 (20)</td>
		</tr>
		<tr>
			<td>Stroke using OAC</td>
			<td>3 (30)</td>
		</tr>
		<tr>
			<td>Patient Preference</td>
			<td>5 (50)</td>
		</tr>
		<tr>
			<td colspan="2">LAA, mm</td>
		</tr>
		<tr>
			<td>LAA width</td>
			<td>20.2+/2.2</td>
		</tr>
		<tr>
			<td>LAA length</td>
			<td>27.9+/-5.4</td>
		</tr>
		<tr>
			<td>Multilobular</td>
			<td>4 (40)</td>
		</tr>
	</tbody>
</table>
Table 1. Baseline characteristics of patients enrolled in the study. Data reprinted with permission from the Netherlands Heart Journal 21. All data are presented as mean +/- standard deviation, as number with percentage (n(%)) or median with lower and upper range, N=number, mm=millimeters, OAC=oral anticoagulation, LAA=left atrial appendage.
<table border="1">
	<tbody>
		<tr>
			<td colspan="2">Procedural characteristics</td>
		</tr>
		<tr>
			<td>PVAC, n(%)</td>
			<td>9 (90)</td>
		</tr>
		<tr>
			<td>Size device (mm)</td>
			<td>24 (21-27)</td>
		</tr>
		<tr>
			<td>21</td>
			<td>1 (10)</td>
		</tr>
		<tr>
			<td>24</td>
			<td>8 (80)</td>
		</tr>
		<tr>
			<td>27</td>
			<td>1 (10)</td>
		</tr>
		<tr>
			<td>LAA Occlusion Time (min)</td>
			<td>56 (38-137)</td>
		</tr>
		<tr>
			<td>Total procedural time (min)</td>
			<td>104 (75-167)</td>
		</tr>
		<tr>
			<td>Devices, n</td>
			<td>2 (1-3)</td>
		</tr>
		<tr>
			<td colspan="2">Complications, n(%)</td>
		</tr>
		<tr>
			<td>Catheter thrombus</td>
			<td>1 (10)*</td>
		</tr>
		<tr>
			<td>Inguinal bleeding</td>
			<td>1 (10)*</td>
		</tr>
		<tr>
			<td>Pericardial effusion</td>
			<td>0 (0)*</td>
		</tr>
		<tr>
			<td colspan="2">TEE</td>
		</tr>
		<tr>
			<td>Successful implantation</td>
			<td>10 (100)</td>
		</tr>
		<tr>
			<td>Residual Flow</td>
			<td>1 (10)</td>
		</tr>
		<tr>
			<td>Hospitalization, days</td>
			<td>2 (2-7)</td>
		</tr>
	</tbody>
</table>
Table 2. Procedural characteristics. Data reprinted with permission from the Netherlands Heart Journal 21. All data are presented as mean +/- standard deviation, as number with percentage (n(%)) or median with lower and upper range, N=number, mm=millimeters, PVAC=pulmonary vein ablation catheter, TEE=Transesophageal echocardiography. *Both in the same patient.
<table border="1">
	<tbody>
		<tr>
			<td colspan="2">Follow-up characteristics at 45 days</td>
		</tr>
		<tr>
			<td>Number, n (%)</td>
			<td>10</td>
		</tr>
		<tr>
			<td>TEE, n (%)</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Residual flow</td>
			<td>3 (33)</td>
		</tr>
		<tr>
			<td>Device embolization</td>
			<td>1 (10)</td>
		</tr>
		<tr>
			<td>Thrombus on device</td>
			<td>0 (0)</td>
		</tr>
		<tr>
			<td>Vitamin K antagonist, n(%)</td>
			<td>6 (67)</td>
		</tr>
		<tr>
			<td colspan="2">Complications during follow-up, n(%)</td>
		</tr>
		<tr>
			<td>Death</td>
			<td>0 (0)</td>
		</tr>
		<tr>
			<td>Stroke or TIA</td>
			<td>0 (0)</td>
		</tr>
		<tr>
			<td>Bleeding</td>
			<td>1 (10)</td>
		</tr>
	</tbody>
</table>
Table 3. 45-day follow-up. Data reprinted with permission from Netherlands Heart Journal 21. All data are presented as mean +/- standard deviation, as number with percentage (n(%)) or median with lower and upper range, N=number, mm=millimeters, TEE=Transesophageal echocardiography, TIA=transient ischemic attack.
<img alt="Figure 1" src="/files/ftp_upload/3818/3818fig1.jpg" /> 
 Figure 1. The WATCHMAN Device. The WATCHMAN device is a nitinol cage covered with a Polyethyl terephthalate membrane. Fixation barbs around the perimeter secure the device in the LAA.
<img alt="Figure 2" src="/files/ftp_upload/3818/3818fig2.jpg" /> 
 Figure 2. A complete LAA isolation or occlusion. TEE with color Doppler demonstrating proper positioning and occlusion at 45 days post-procedure from different TEE-angulations in a single patient. As the TEE probe looks at the device between 0-180 &deg;, there is no flow in the appendage and that the device is appropriately placed in all views.
<img alt="Figure 3" src="/files/ftp_upload/3818/3818fig3.jpg" /> 
 Figure 3. An incomplete LAA occlusion. TEE with color Doppler 45 days post-procedure demonstrating flow (leak) around the device. As the TEE probe looks at the device between 0-180 &deg;, a small gap (&lt;5 mm) with color flow is seen around the device. These findings still can be qualified as successful sealing. Therefore OAC can be stopped. All images are from the same patient.

Watch this Scientific Journal Video about Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation at JoVE.com

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation

导管消融术相结合，与安置的WATCHMAN左心耳封堵装置，以防止在非瓣膜性房颤患者缺血性中风。

结合消融房颤左心耳封堵

Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia, affecting millions of individuals worldwide 1-3. The rapid, irregular, and ...

catheter-ablation-combination-with-left-atrial-appendage-closure-for

Research

JoVE Journal

Medicine

33.1K 次观看.  St. Antonius Hospital, The Netherlands. 导管消融术相结合，与安置的WATCHMAN左心耳封堵装置，以防止在非瓣膜性房颤患者缺血性中风。

视频: Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation

High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation

Atrial fibrillation (AF) is a complex cardiac arrhythmia with high morbidity and mortality.1,2 It is the most common sustained cardiac rhythm disturbance seen in clinical practice and its prevalence is expected to increase in the coming years.3 Increased intra-atrial pressure and dilatation have been long recognized to lead to AF,1,4 which highlights the relevance of using animal models and stretch to study AF dynamics. Understanding the mechanisms underlying AF requires visualization of the cardiac electrical waves with high spatial and temporal resolution. While high-temporal resolution can be achieved by conventional electrical mapping traditionally used in human electrophysiological studies, the small number of intra-atrial electrodes that can be used simultaneously limits the spatial resolution and precludes any detailed tracking of the electrical waves during the arrhythmia. The introduction of optical mapping in the early 90's enabled wide-field characterization of fibrillatory activity together with sub-millimeter spatial resolution in animal models5,6 and led to the identification of rapidly spinning electrical wave patterns (rotors) as the sources of the fibrillatory activity that may occur in the ventricles or the atria.7-9 Using combined time- and frequency-domain analyses of optical mapping it is possible to demonstrate discrete sites of high frequency periodic activity during AF, along with frequency gradients between left and right atrium. The region with fastest rotors activates at the highest frequency and drives the overall arrhythmia.10,11 The waves emanating from such rotor interact with either functional or anatomic obstacles in their path, resulting in the phenomenon of fibrillatory conduction.12 Mapping the endocardial surface of the posterior left atrium (PLA) allows the tracking of AF wave dynamics in the region with the highest rotor frequency. Importantly, the PLA is the region where intracavitary catheter-based ablative procedures are most successful terminating AF in patients,13 which underscores the relevance of studying AF dynamics from the interior of the left atrium. Here we describe a sheep model of acute stretch-induced AF, which resembles some of the characteristics of human paroxysmal AF. Epicardial mapping on the left atrium is complemented with endocardial mapping of the PLA using a dual-channel rigid borescope c-mounted to a CCD camera, which represents the most direct approach to visualize the patterns of activation in the most relevant region for AF maintenance.

This report provides a detailed description of the methodology and results of simultaneous endocardial and epicardial optical mapping of electrical excitation in the intact left atrium of a Langendorff-perfused sheep heart during stretch-induced atrial fibrillation.

高分辨率的心内膜和心外膜光学在拉伸诱发心房颤动的羊模型的映射

 Atrial fibrillation (AF) is a complex cardiac arrhythmia with high morbidity and mortality.1,2 It is the most common sustained cardiac rhythm disturbance ...

科研

医学

The WATCHMAN Left Atrial Appendage Closure Device for Atrial Fibrillation

Atrial fibrillation (AF) is the most common cardiac arrhythmia, affecting an estimated 6 million people in the United States 1. Since AF affects primarily elderly people, its prevalence increases parallel with age. As such, it is expected that 15.9 million Americans will be affected by the year 2050 2. Ischemic stroke occurs in 5% of non-anticoagulated AF patients each year. Current treatments for AF include rate control, rhythm control and prevention of stroke 3.
The American College of Cardiology, American Heart Association, and European Society of Cardiology currently recommended rate control as the first course of therapy for AF 3. Rate control is achieved by administration of pharmacological agents, such as &beta;-blockers, that lower the heart rate until it reaches a less symptomatic state 3. Rhythm control aims to return the heart to its normal sinus rhythm and is typically achieved through administration of antiarrhythmic drugs such as amiodarone, electrical cardioversion or ablation therapy. Rhythm control methods, however, have not been demonstrated to be superior to rate-control methods 4-6. In fact, certain antiarrhythmic drugs have been shown to be associated with higher hospitalization rates, serious adverse effects 3, or even increases in mortality in patients with structural heart defects 7. Thus, treatment with antiarrhythmics is more often used when rate-control drugs are ineffective or contraindicated. Rate-control and antiarrhythmic agents relieve the symptoms of AF, including palpitations, shortness of breath, and fatigue 8, but don't reliably prevent thromboembolic events 6.
Treatment with the anticoagulant drug warfarin significantly reduces the rate of stroke or embolism 9,10. However, because of problems associated with its use, fewer than 50% of patients are treated with it. The therapeutic dose is affected by drug, dietary, and metabolic interactions, and thus requires detailed monitoring. In addition, warfarin has the potential to cause severe, sometimes lethal, bleeding 2. As an alternative, aspirin is commonly prescribed. While aspirin is typically well tolerated, it is far less effective at preventing stroke 10. Other alternatives to warfarin, such as dabigatran 11 or rivaroxaban 12 demonstrate non-inferiority to warfarin with respect to thromboembolic events (in fact, dabigatran given as a high dose of 150 mg twice a day has shown superiority). While these drugs have the advantage of eliminating dietary concerns and eliminating the need for regular blood monitoring, major bleeding and associated complications, while somewhat less so than with warfarin, remain an issue 13-15.
Since 90% of AF-associated strokes result from emboli that arise from the left atrial appendage (LAA) 2, one alternative approach to warfarin therapy has been to exclude the LAA using an implanted device to trap blood clots before they exit. Here, we demonstrate a procedure for implanting the WATCHMAN Left Atrial Appendage Closure Device. A transseptal cannula is inserted through the femoral vein, and under fluoroscopic guidance, inter-atrial septum is crossed. Once access to the left atrium has been achieved, a guidewire is placed in the upper pulmonary vein and the WATCHMAN Access Sheath and dilator are advanced over the wire into the left atrium. The guidewire is removed, and the access sheath is carefully advanced into the distal portion of the LAA over a pigtail catheter. The WATCHMAN Delivery System is prepped, inserted into the access sheath, and slowly advanced. The WATCHMAN device is then deployed into the LAA. The device release criteria are confirmed via fluoroscopy and transesophageal echocardiography (TEE) and the device is released.

The accompanying video describes a procedure for percutaneous placement of the WATCHMAN Left Atrial Appendage (LAA) Device. The WATCHMAN is a nitinol device designed to be permanently implanted at, or slightly distal to, the opening of the left atrial appendage (LAA) to trap blood clots before they exit the LAA, preventing thromboembolic stroke.

守望的左心耳封闭装置心房颤动

Atrial fibrillation (AF) is the most common cardiac arrhythmia, affecting an estimated 6 million people in the United States 1. Since AF affects primarily ...

Robotic Ablation of Atrial Fibrillation

Background: Pulmonary vein isolation (PVI) is an established treatment for atrial fibrillation (AF). During PVI an electrical conduction block between pulmonary vein (PV) and left atrium (LA) is created. This conduction block prevents AF, which is triggered by irregular electric activity originating from the PV. However, transmural atrial lesions are required which can be challenging. Re-conduction and AF recurrence occur in 20 - 40% of the cases. Robotic catheter systems aim to improve catheter steerability. Here, a procedure with a new remote catheter system (RCS), is presented. Objective of this article is to show feasibility of robotic AF ablation with a novel system. Materials and Methods: After interatrial trans-septal puncture is performed using a long sheath and needle under fluoroscopic guidance. The needle is removed and a guide wire is placed in the left superior PV. Then an ablation catheter is positioned in the LA, using the sheath and wire as guide to the LA. LA angiography is performed over the sheath. A circular mapping catheter is positioned via the long sheath into the LA and a three-dimensional (3-D) anatomical reconstruction of the LA is performed. The handle of the ablation catheter is positioned in the robotic arm of the Amigo system and the ablation procedure begins. During the ablation procedure, the operator manipulates the ablation catheter via the robotic arm with the use of a remote control. The ablation is performed by creating point-by-point lesions around the left and right PV ostia. Contact force is measured at the catheter tip to provide feedback of catheter-tissue contact. Conduction block is confirmed by recording the PV potentials on the circular mapping catheter and by pacing maneuvers. The operator stays out of the radiationfield during ablation. Conclusion: The novel catheter system allows ablation with high stability on low operator fluoroscopy exposure.

Pulmonary vein isolation (PVI) with an ablation catheter is a curative treatment for atrial fibrillation (AF). Robotic catheter systems aim to improve catheter steerability. Here, a procedure with a new robotic catheter system is presented. The goal of the procedure is electrical block between pulmonary vein and left atrium.

房颤消融机器人

Background: Pulmonary vein isolation (PVI) is an established treatment for atrial fibrillation (AF). During PVI an electrical conduction block between ...

Reduction of Iatrogenic Atrial Septal Defects with an Anterior and Inferior Transseptal Puncture Site when Operating the Cryoballoon Ablation Catheter

The cryoballoon catheter ablates atrial fibrillation (AF) triggers in the left atrium (LA) and pulmonary veins (PVs) via transseptal access. The typical transseptal puncture site is the fossa ovalis (FO) &#8211; the atrial septum&#8217;s thinnest section. A potentially beneficial transseptal site, for the cryoballoon, is near the inferior limbus (IL). This study examines an alternative transseptal site near the IL, which may decrease the frequency of acute iatrogenic atrial septal defect (IASD). Also, the study evaluates the acute pulmonary vein isolation (PVI) success rate utilizing the IL location. 200 patients were evaluated by retrospective chart review for acute PVI success rate with an IL transseptal site. An additional 128 IL transseptal patients were compared to 45 FO transseptal patients by performing Doppler intracardiac echocardiography (ICE) post-ablation to assess transseptal flow after removal of the transseptal sheath. After sheath removal and by Doppler ICE imaging, 42 of 128 (33%) IL transseptal patients demonstrated acute transseptal flow, while 45 of 45 (100%) FO transseptal puncture patients had acute transseptal flow. The difference in acute transseptal flow detection between FO and IL sites was statistically significant (P &#60;0.0001). Furthermore, 186 of 200 patients (with an IL transseptal puncture) did not need additional ablation(s) and had achieved an acute PVI by a &#8220;cryoballoon only&#8221; technique. An IL transseptal puncture site for cryoballoon AF ablations is an effective location to mediate PVI at all four PVs. Additionally, an IL transseptal location can lower the incidence of acute transseptal flow by Doppler ICE when compared to the FO. Potentially, the IL transseptal site may reduce later IASD complications post-cryoballoon procedures.

The goal of this study is to demonstrate the preferential location of transseptal puncture during a cryoballoon catheter ablation procedure for the treatment of atrial fibrillation.

与前伪劣间隔穿刺网站减少医源性房间隔缺损操作Cryoballoon消融导管时，

The cryoballoon catheter ablates atrial fibrillation (AF) triggers in the left atrium (LA) and pulmonary veins (PVs) via transseptal access. The typical ...

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

In this article, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes (ACMs) from a rat model of metabolic syndrome (MetS)-related heart failure with preserved ejection fraction (HFpEF). The prevalence of MetS-related HFpEF is rising, and atrial cardiomyopathies associated with atrial remodeling and atrial fibrillation are clinically highly relevant as atrial remodeling is an independent predictor of mortality. Studies with isolated single-cell cardiomyocytes are frequently used to corroborate and complement in vivo findings. Circulatory vessel rarefication and interstitial tissue fibrosis pose a potentially limiting factor for the successful single-cell isolation of ACMs from animal models of this disease.
We have addressed this issue by employing a device capable of manually regulating the intraluminal pressure of cardiac cavities during the isolation procedure, substantially increasing the yield of morphologically and functionally intact ACMs. The acquired cells can be used in a variety of different experiments, such as cell culture and functional Calcium imaging (i.e., excitation-contraction-coupling).
We provide the researcher with a step-by-step protocol, a list of optimized solutions, thorough instructions to prepare the necessary equipment, and a comprehensive troubleshooting guide. While the initial implementation of the procedure might be rather difficult, a successful adaptation will allow the reader to perform state-of-the-art ACM isolations in a rat model of MetS-related HFpEF for a broad spectrum of experiments.

Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.

代谢综合征心力衰竭模型大鼠心房心肌细胞的分离与保留射血分数的关系

In this article, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes (ACMs) from a rat model of ...

Left Atrial Stenosis Induced Pulmonary Venous Arterialization and Group 2 Pulmonary Hypertension in Rat

The mechanism of mitral stenosis-induced pulmonary venous arterialization and group 2 pulmonary hypertension (PH) is unclear. There is no rodent model of group 2 PH, due to mitral stenosis (MS), to facilitate the investigation of disease mechanisms and potential therapeutic strategies. We present a novel rat model of pulmonary venous congestion-induced pulmonary venous arterialization and group 2 PH caused by left atrial stenosis (LAS). LAS is achieved by constricting the left atrium using a half-closed titanium clip. After the LAS surgery, a rat model with a transmitral inflow velocity greater than or equal to 2.0 m/s on echocardiography gradually develops pulmonary venous arterialization and group 2 PH over an 8- to 10-week period. In this protocol, we provide the step-by-step procedure of how to perform the LAS surgery. The presented LAS rat model mimics MS in humans and is useful for studying the underlying molecular mechanism of pulmonary venous arterialization and for the preclinical evaluation of therapies for group 2 PH.

Left atrial stenosis (LAS) is a novel surgical technique used for studying group 2 pulmonary hypertension (PH) and mechanisms underlying pulmonary venous arterialization. Here, we present a protocol to constrict the left atrium using a titanium clip to cause pulmonary venous arterialization and moderate PH in a rat.

左心房狭窄致大鼠肺静脉动脉化及第2组肺动脉高压

The mechanism of mitral stenosis-induced pulmonary venous arterialization and group 2 pulmonary hypertension (PH) is unclear. There is no rodent model of ...

Cooling or Warming the Esophagus to Reduce Esophageal Injury During Left Atrial Ablation in the Treatment of Atrial Fibrillation

Ablation of the left atrium using either radiofrequency (RF) or cryothermal energy is an effective treatment for atrial fibrillation (AF) and is the most frequent type of cardiac ablation procedure performed. Although generally safe, collateral injury to surrounding structures, particularly the esophagus, remains a concern. Cooling or warming the esophagus to counteract the heat from RF ablation, or the cold from cryoablation, is a method that is used to reduce thermal esophageal injury, and there are increasing data to support this approach. This protocol describes the use of a commercially available esophageal temperature management device to cool or warm the esophagus to reduce esophageal injury during left atrial ablation. The temperature management device is powered by standard water-blanket heat exchangers, and is shaped like a standard orogastric tube placed for gastric suctioning and decompression. Water circulates through the device in a closed-loop circuit, transferring heat across the silicone walls of the device, through the esophageal wall. Placement of the device is analogous to the placement of a typical orogastric tube, and temperature is adjusted via the external heat-exchanger console.

The goal of this protocol is to describe the use of esophageal temperature modulation to counteract esophageal thermal injury from left atrial ablation for the treatment of atrial fibrillation.

在心房颤动治疗中，为食道冷却或加热，以减少左心房消融期间的食管损伤

Ablation of the left atrium using either radiofrequency (RF) or cryothermal energy is an effective treatment for atrial fibrillation (AF) and is the most ...

Echocardiographic Evaluation of Atrial Communications before Transcatheter Closure

Transthoracic (TTE) and transesophageal echocardiography (TEE) is the standard imaging method for atrial septal defect (ASD) and patent foramen ovale (PFO) detection, for patient selection for transcatheter ASD/PFO closure,&#160;for intraoperative guidance and for long-term follow-up. The size, shape, location and the number of the atrial communications schould be determined. The accuracy of PFO detection can be improved by using agitated saline together with maneuvers to transiently increase the right atrial (RA) pressure. The appearance of microbubbles in the left atrium (LA) within 3 cardiac cycles after opacification of the RA is considered positive for the presence of an intracardiac shunt. Three dimensional TEE identifies further septal fenestrations and describes the dynamic morphology of ASD/PFO and atrial septal aneurysm. Follow-up evaluations with TTE is recommended at 1, 6, and 12 months after the procedure, with a subsequent evaluation every year. Previous studies showed an increased incidence of atrial arrhythmias early after device closure. Speckle tracking analysis may help to understand functional left atrial remodeling following percutaneous closure and its impact on atrial arrhythmias.

Transthoracic (TTE) and transesophageal (TEE) echocardiography represent the basic imaging tools for interatrial septum examination. Three dimensional (3D) TEE provides incremental information in the assessment of the interatrial septum. Further advanced echocardiography techniques using speckle tracking echocardiography are applied for sensitive volumetric and functional assessment of the heart chambers.&#160;

经导管闭合前心房通路的超声心动图评估

Transthoracic (TTE) and transesophageal echocardiography (TEE) is the standard imaging method for atrial septal defect (ASD) and patent foramen ovale ...

Sterile Pericarditis in Aachener Minipigs As a Model for Atrial Myopathy and Atrial Fibrillation

Atrial fibrillation (AF) is the most common arrhythmia caused by structural remodeling of the atria, also called atrial myopathy. Current therapies only target the electrical abnormalities and not the underlying atrial myopathy. For the development of novel therapies, a reproducible large animal model of atrial myopathy is necessary. This paper presents a model of sterile pericarditis-induced atrial myopathy in Aachener minipigs. Sterile pericarditis was induced by spraying sterile talcum and leaving a layer of sterile gauze over the atrial epicardial surface. This led to inflammation and fibrosis, two crucial components of the pathophysiology of atrial myopathy, making the atria susceptible to the induction of AF. Two pacemaker electrodes were positioned epicardially on each atrium and connected to two pacemakers from different manufacturers. This strategy allowed for repeated non-invasive atrial programmed stimulation to determine the inducibility of AF at specified time points after surgery. Different protocols to test AF inducibility were used. The advantages of this model are its clinical relevance, with AF inducibility and the rapid induction of inflammation and fibrosis-both present in atrial myopathy-and its reproducibility. The model will be useful in the development of novel therapies targeting atrial myopathy and AF.

We describe a sterile pericarditis model in minipigs to study atrial myopathy and atrial fibrillation (AF). We present surgical and anesthetic techniques, strategies for vascular access, and a protocol to study the inducibility of AF.

Aachener Minipigs的无菌性心包炎作为心房肌病和心房颤动的模型

Atrial fibrillation (AF) is the most common arrhythmia caused by structural remodeling of the atria, also called atrial myopathy. Current therapies only ...

Transesophageal Atrial Burst Pacing for Atrial Fibrillation Induction in Rats

Animal studies have brought important insights into our understanding regarding atrial fibrillation (AF) pathophysiology and therapeutic management. Reentry, one of the main mechanisms involved in AF pathogenesis, requires a certain mass of myocardial tissue in order to occur. Due to the small size of the atria, rodents have long been considered 'resistant' to AF. Although spontaneous AF has been shown to occur in rats, long-term follow-up (up to 50 weeks) is required for the arrhythmia to occur in those models. The present work describes an experimental protocol of transesophageal atrial burst pacing for rapid and efficient induction of AF in rats. The protocol can be successfully used in rats with healthy or remodeled hearts, in the presence of a wide variety of risk factors, allowing the study of AF pathophysiology, identification of novel therapeutic targets, and evaluation of novel prophylactic and/or therapeutic strategies.

The present work describes an experimental protocol of transesophageal atrial burst pacing for efficient induction of atrial fibrillation (AF) in rats. The protocol can be used in rats with healthy or remodeled hearts, allowing the study of AF pathophysiology, identification of novel therapeutic targets, and evaluation of new therapeutic strategies.

经食管心房爆破起搏在大鼠心房颤动诱导中的应用

Animal studies have brought important insights into our understanding regarding atrial fibrillation (AF) pathophysiology and therapeutic management. ...