KIST格兰特（2E21510），教育部国家荣誉的科学家计划，并通过韩国国家研究基金会原有的技术为脑科学的研究计划，由教育部，科学和技术（2010-0018944）资助支持。

1。摘要只C57BL/6J - 129S4/SvJae混合小鼠（12〜15周龄）麻醉与氯胺酮（120毫克/公斤的IP）和甲苯噻嗪（6毫克/公斤，IP）的鸡尾酒。尾巴或脚趾捏之前检查手术麻醉深度。鼠标是固定的立体定位仪（大卫Kopf仪器，型号902，加州，美国）。头皮切开约25毫米利多卡因减轻疼痛的治疗颅骨暴露在中线。任何头骨碎片是被盐水浸泡过的棉花提示，以提高电极坚持头骨。前囟门和lambda点标记，并在同一水平面。聚酰亚胺为基础的微电极阵列（矿务局阵列）的位置，所以，它的垂直中线符合的头骨中线。前囟门点应设在第 5层的前矿务局阵列中间。使用两个或三个microscrews在边缘的头骨，以帮助确保电极用牙科水泥的位置空空间。为支撑矿务局阵列连接器，我们对头部的中间支撑位置，并用牙科水泥覆盖。固化后，切开皮肤缝合，至少5天的恢复。在恢复过程中，老鼠被安置在个别笼免费获得食物和水。恢复后，进行脑电记录。动物护理，外科手术，并记录程序，按照机构的动物护理和使用委员会的指引，继1992年的韩国实验室动物保护条例"和相关准则的法。在这个手稿矿务局阵列制备蔡描述。等。人请联系作者在视频使用的相同的PBM阵列。 2。程序<ol><li>准备消毒的手术器械和立体定位仪。 </li><li>麻醉与麻醉（例如，氯胺酮/甲苯​​噻嗪，剂量为120和60毫克/公斤的IP，repectively）鼠标。尾巴或脚趾捏之前检查手术麻醉深度。 </li><li>立体定位仪安装到鼠标放入鼠标的耳道，耳酒吧，并拧紧到位。眼睛可覆盖生物相容性果冻（如凡士林），以防止眼睛干燥，手术灯。保湿鼠标的头部，用70％乙醇后，剃光头皮毛。 </li><li>切开头皮约25毫米的头部。这是可取的，适用2％的少量利多卡因头皮下的盐酸。捏皮肤微夹钳（18052-03，精细的科学工具（美国），公司​​，加州，美国），以保持切口敞开。 </li><li>擦拭用盐水浸泡棉签提高矿务局阵列坚持颅骨其余的头骨上的任何组织碎片。使用去除膜的残留过氧化氢也是有效的。 </li><li>前囟和lambda点上找到的头骨，并与石油为基础的记号笔标记。矿务局阵列，所以，它的垂直中线符合图1所示的头骨中线放置。矿务局阵列的前5次用一滴自来水层中间位置的前囟门。从内侧向外侧，为更好地遵守仔细擦拭薄膜电极。为了帮助薄膜电极薄膜电极坚持严格的头骨，应完全干燥的头骨。薄膜电极仍然被固定在头骨上的范德华力。重新定位电极，另一滴自来水是必要的，以防止被撕裂的电极。 <img src="/files/ftp_upload/2562/2562fig1.jpg" alt="图1" /> 图1（左）鼠标头骨矿务局阵列。矿务局阵列anterio后轴相匹配的前囟（BP）和lambda点（LP）之间的线。 （右）上公认的鼠脑表面电极位置。鼠标的解剖结构分割的3 - D的磁共振成像数字地图集在布鲁克海文国家实验室和电极位置生产的成年C57BL/6J小鼠脑数据库，根据立体测量标记。地线（G）和参考（REF）电极标记在右边的图。 </li><li>标记至少有三个开放区域的头骨上的位置，然后在这些地点使用支持microscrews定位一个牙钻的孔。这些microscrews的stanchion作用和机械支持的头骨上的牙科水泥。在钻井过程中需要格外小心。由于小鼠颅骨的薄层组成，出血可发生在钻井过程中轻松。出血发生时，停钻成孔止血粉少量申请。 </li><li>应用牙科丙烯酸水泥（牙科顶点，顶点自固化，荷兰），覆盖的PBM的阵列，锚钉，而其余的头骨。准备在胶和粘性彗星的牙科水泥ondition。 </li><li>一个刚性棒作为一个支柱上使用强力胶头（例如，乐泰，汉高乐泰公司，都柏林，爱尔兰）的中间位置。放在后面的连接器平面双面胶带。操纵矿务局阵列连接器连接支柱的一角。头骨和连接器之间的垂直距离应超过5毫米。棒连接器的平面和提示使用强力胶水粘更好的附着力。 </li><li>确保彻底治愈，牙科水泥。然后申请在头骨的牙齿丙烯酸水泥和安全颅骨的连接器连接器平面的背面。 </li><li>删除微夹钳。缝合切开皮肤消毒缝合纤维。从设备中取出鼠标。你可以申请额外的牙科丙烯酸水泥盾暴露颅骨。周围的水泥和缝合皮肤应用抗生素（fucidin软膏，东和制药，韩国）。 </li><li>鼠标放置在消毒笼，直到它恢复其原来的体重（约5至7天）。建议不作任何笼交配。 </li><li>恢复后，由一个轻量级的印刷电路板（PCB）连接器连接到一个多通道脑电放大器系统，在自己的笼子里（Synamps 2），美国北卡罗莱纳州的流浆箱的连接鼠标。连接线应慎重对待，因此它不纠缠不清。 </li><li>经过1小时的习惯，进行脑电记录。扫描4.4。 （美国北卡罗莱纳州）在本实验中使用采集软件。一个典型的时间跟踪鼠标脑电图的设置如图2所示。建议记录的视频与鼠标的行为。 </li><li> MATLAB（MathWorks公司，纳提克，马萨诸塞州，美国）获得的信号可以分析产生的地形脑图。鼠脑的脑电功率图的示例拓扑图如图3所示。 </li></ol> 3。代表性的成果如果上述手术过程中正确地进行，将成功地执行了40个频道的鼠标脑电记录，无不良渠道。下图显示的代表性原始脑电图跟踪和地形图。 <img src="/files/ftp_upload/2562/2562fig2.jpg" alt="图2" /> 图2:一个脑电图经历的情况下通过注射γ-丁内酯（GBL，经口50mg/kg，IP）致痫样本跟踪。红色条表示在失神发作棘波放电（SWDS）。水平和垂直轴表示时间和电压分别。在图中定义的通道蒙太奇数如下。 1（右）。 <img src="/files/ftp_upload/2562/2562fig3.jpg" alt="图3" /> 图3。地形图显示2-6赫兹配电。在彩条的值代表的势力地图的颜色规模。在棘波和γ-丁内酯（GBL，经口50mg/kg，IP），大额顶皮层区域的力量诱导显着的排放（SWDS）相比增加GBL注射前（基线）的权力。

<ol>
	<li>Choi, J. H., Koch, K. P., Poppendieck, W., Lee, M., Shin, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+resolution+Electroencephalography+in+Freely+Moving+Mice.">High resolution Electroencephalography in Freely Moving Mice.</a> J Neurophysiol. , .</li><li>Lee, M., Shin, H. S., Choi, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+recording+of+brain+activity+and+functional+connectivity+in+the+mouse+brain.">Simultaneous recording of brain activity and functional connectivity in the mouse brain.</a> Conf Proc IEEE Eng Med Biol Soc. , 2934-2936 (2009).</li></ol>

在这里，我们报告，手术及录音的程序，以获得自由活动的小鼠高密度的脑电图。这种方法使我们获得脑功能的地图，从小鼠模型，研究自发的有节奏的1,2或事件相关脑活动的分子机制。 矿务局阵列中的所有渠道收购是一个必要条件，以获得整个大脑映射，但是为每个通道的安全接触是非常具有挑战性的。为了保持一致的联系渠道颅骨，下面的程序是重要的。首先，任何组织头骨碎片可能会设置除了从头骨的电极。因此，它是非常重要的，以消除所有的组织碎片，并用消毒棉签干颅骨表面。其次，矿务局阵列的能力，能坚持到头骨表面的范德华力。建议申请前申请矿务局阵列头骨，头骨建立水层下降的自来水。第三，正确的牙科水泥粘度是非常重要的。如果是过流鼻涕，有可能建立一个绝缘层，下矿务局阵列。如果过于密集，牙科水泥将很容易从头骨，由于其自​​身的重量土崩瓦解。最后，至少有三个配套microscrews应植入的头骨，使牙科水泥紧密结合的头骨。支持microscrews托起牙科水泥和安全的探头，这是必不可少的长期研究的。 由于其尺寸和重量成年小鼠的慢性研究矿务局阵列的应用将受到限制。根据我们的经验，超过25克重的老鼠已经足够使用。作为进一步的应用，通过我们矿务局阵列中的分支之间的空空间，可能引入二次电极记录或刺激的目的。

<table><tbody><tr><td>&amp;#947;butyrolactone</td><td>Sigma-Aldrich</td><td>H7629-500G</td><td></td></tr></tbody></table>

high-density eeg recordings of the freely moving mice using polyimide-based microelectrode

脑电图（EEG）显示的一个大型神经元群体的平均的电活动。它被广泛用来作为一种无创性脑认知神经科学的监测工具以及神经内科癫痫和睡眠障碍的诊断工具。然而，脑电图节律产生的基本机制仍然是下的面纱。最近推出的聚酰亚胺为基础的微电极脑电图 1高分辨率鼠标（矿务局阵列）的试验之一，回答基于丰富的遗传资源，在小鼠模型包含复杂的脑电图生成过程分析脑电信号的神经生理问题。这nanofabricated矿务局阵列对小鼠的头骨中的应用是一个有效的工具，收集大型的转基因小鼠的大脑活动和容纳结合与行为，以确定一定的脑电节律的神经关联。但它的超薄厚度和分叉结构导致矿务局阵列处理和植入的麻烦。在视频中，矿务局阵列鼠标头骨植入的准备和手术步骤描述一步一步来。还提供了处理和手术技巧，以帮助研究人员成功植入。

Watch this Scientific Journal Video about High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode at JoVE.com

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

在这篇文章中，我们介绍了手术过程和处理超薄的聚酰亚胺为基础的微电极阵列（PBM阵列）收购的高密度脑电图（EEG）在小鼠模​​型脑照相鼠标颅骨植入的提示。

高密度的自由移动，使用基于聚酰亚胺的微电极的小鼠的脑电图记录

Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a ...

high-density-eeg-recordings-freely-moving-mice-using-polyimide-based

Nanyang Technological University

Research

JoVE Journal

Neuroscience

36.9K 次观看.  Korea Institute of Science and Technology (KIST). 在这篇文章中，我们介绍了手术过程和处理超薄的聚酰亚胺为基础的微电极阵列（PBM阵列）收购的高密度脑电图（EEG）在小鼠模​​型脑照相鼠标颅骨植入的提示。

视频: High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic targeting for microcircuit dissection and disease modeling. The introduction of optogenetics, for example, has allowed for bidirectional manipulation of genetically-identified neurons, at an unprecedented temporal resolution. To capitalize on these tools and gain insight into dynamic interactions among brain microcircuits, it is essential that one has the ability to record from ensembles of neurons deep within the brain of this small rodent, in both head-fixed and freely behaving preparations. To record from deep structures and distinct cell layers requires a preparation that allows precise advancement of electrodes towards desired brain regions. To record neural ensembles, it is necessary that each electrode be independently movable, allowing the experimenter to resolve individual cells while leaving neighboring electrodes undisturbed. To do both in a freely behaving mouse requires an electrode drive that is lightweight, resilient, and highly customizable for targeting specific brain structures.
A technique for designing and fabricating miniature, ultralight weight, microdrive electrode arrays that are individually customizable and easily assembled from commercially available parts is presented. These devices are easily scalable and can be customized to the structure being targeted; it has been used successfully to record from thalamic and cortical regions in a freely behaving animal during natural behavior.

Understanding the neural substrates of behavior requires brain circuit ensemble recording. Because of its genetic tractability, the mouse offers a model for circuit dissection and disease mimicry. Here, a method of designing and fabricating miniaturized probes is described that is suitable for targeting deep brain structure in the mouse.

设计和超轻重量的制作，可调节的多电极电生理记录小鼠探头

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic ...

科研

神经科学

Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology

Implantable brain electrophysiology probes are valuable tools in neuroscience due to their ability to record neural activity with high spatiotemporal resolution from shallow and deep brain regions. Their use has been hindered, however, by mechanical and structural mismatches between the probes and brain tissue that commonly lead to micromotion and gliosis with resulting signal instability in chronic recording experiments. In contrast, following the implantation of ultraflexible mesh electronics via syringe injection, the mesh probes form a seamless, gliosis-free interface with the surrounding brain tissue that enables stable tracking of individual neurons on at least a year timescale. This protocol details the key steps in a typical mouse neural recording experiment using syringe-injectable mesh electronics, including the fabrication of mesh electronics in a standard photolithography-based process possible at many universities, loading mesh electronics into standard capillary needles, stereotaxic injection in vivo, connection of the mesh input/output to standard instrumentation interfaces, restrained or freely moving recording sessions, and histological sectioning of brain tissue containing mesh electronics. Representative neural recordings and histology data are presented. Investigators familiar with this protocol will have the knowledge necessary to incorporate mesh electronics into their own experiments and take advantage of the unique opportunities afforded by long-term stable neural interfacing, such as studies of aging processes, brain development, and the pathogenesis of brain disease.

Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.

注射器-注射用网状电子技术稳定的慢性啮齿动物电生理学

Implantable brain electrophysiology probes are valuable tools in neuroscience due to their ability to record neural activity with high spatiotemporal ...

生物工程

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

Differences in the activity of neurotransmitters and neuromodulators, and consequently different neural responses, can be found between anesthetized and awake animals. Therefore, methods allowing the manipulation of synaptic systems in awake animals are required in order to determine the contribution of synaptic inputs to neuronal processing unaffected by anesthetics. Here, we present methodology for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. By combining these procedures, we performed microiontophoretic injections of gabazine to selectively block GABAA receptors in neurons of the inferior colliculus of head-restrained mice. Gabazine successfully modified neural response properties such as the frequency response area and stimulus-specific adaptation. Thus, we demonstrate that our methods are suitable for recording single-unit activity and for dissecting the role of specific neurotransmitter receptors in auditory processing.
The main limitation of the described procedure is the relatively short recording time (~3 hr), which is determined by the level of habituation of the animal to the recording sessions. On the other hand, multiple recording sessions can be performed in the same animal. The advantage of this technique over other experimental procedures used to manipulate the level of neurotransmission or neuromodulation (such as systemic injections or the use of optogenetic models), is that the drug effect is confined to the local synaptic inputs to the target neuron. In addition, the custom-manufacture of electrodes allows adjustment of specific parameters according to the neural structure and type of neuron of interest (such as the tip resistance for improving the signal-to-noise ratio of the recordings).

We present methods for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. This technique allows the detailed analysis of putative local synaptic inputs to the neuron of interest.

神经元电活动外记录与神经活性物质在清醒小鼠微电泳应用相结合

Differences in the activity of neurotransmitters and neuromodulators, and consequently different neural responses, can be found between anesthetized and ...

High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources

Advanced electroencephalographic analysis techniques requiring high spatial resolution, including electrical source imaging and measures of network connectivity, are applicable to an expanding variety of questions in neuroscience. Performing these kinds of analyses in a rodent model requires higher electrode density than traditional screw electrodes can accomplish. While higher-density electroencephalographic montages for rodents exist, they are of limited availability to most researchers, are not robust enough for repeated experiments over an extended period of time, or are limited to use in anesthetized rodents.1-3 A proposed low-cost method for constructing a durable, high-count, transcranial electrode array, consisting of bilaterally implantable headpieces is investigated as a means to perform advanced electroencephalogram analyses in mice or rats.

Procedures for headpiece fabrication and surgical implantation necessary to produce high signal to noise, low-impedance electroencephalographic and electromyographic signals are presented. While the methodology is useful in both rats and mice, this manuscript focuses on the more challenging implementation for the smaller mouse skull. Freely moving mice are only tethered to cables via a common adapter during recording. One version of this electrode system that includes 26 electroencephalographic channels and 4 electromyographic channels is described below.

Instructions for the low-cost construction and surgical implantation of a chronic transcranial high-density electroencephalographic montage into mice are provided. Signal recording, extraction, and processing techniques are also described.

高密度的脑电图采集在啮齿动物模型使用低成本的开源资源

Advanced electroencephalographic analysis techniques requiring high spatial resolution, including electrical source imaging and measures of network ...

Visual Evoked Potential Recordings in Mice Using a Dry Non-invasive Multi-channel Scalp EEG Sensor

For scalp EEG research environments with laboratory mice, we designed a dry-type 16 channel EEG sensor which is non-invasive, deformable, and re-usable because of the plunger-spring-barrel structural facet and mechanical strengths resulting from metal materials. The whole process for acquiring the VEP responses in vivo from a mouse consists of four steps: (1) sensor assembly, (2) animal preparation, (3) VEP measurement, and (4) signal processing. This paper presents representative measurements of VEP responses from multiple mice with a submicro-voltage signal resolution and sub-hundred millisecond temporal resolution. Although the proposed method is safer and more convenient compared to other previously reported animal EEG acquiring methods, there are remaining issues including how to enhance the signal-to-noise ratio and how to apply this technique with freely moving animals. The proposed method utilizes easily available resources and shows a repetitive VEP response with a satisfactory signal quality. Therefore, this method could be utilized for longitudinal experimental studies and reliable translational research exploiting non-invasive paradigms.

We designed a dry-type 16 channel EEG sensor which is non-invasive, deformable, and re-usable. This paper describes the whole process from manufacturing the proposed EEG electrode to signal processing of visual evoked potential (VEP) signals measured on a mouse scalp using a dry non-invasive multi-channel EEG sensor.

干无创多通道头皮脑电传感器在小鼠视觉诱发电位记录中的应用

For scalp EEG research environments with laboratory mice, we designed a dry-type 16 channel EEG sensor which is non-invasive, deformable, and re-usable ...

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Temporal lobe epilepsy (TLE) is the most common partial complex epileptic syndrome and the least responsive to medications. Deep brain stimulation (DBS) is a promising approach when pharmacological treatment fails or neurosurgery is not recommended. Acute brain slices coupled to microelectrode arrays (MEAs) represent a valuable tool to study neuronal network interactions and their modulation by electrical stimulation. As compared to conventional extracellular recording techniques, they provide the added advantages of a greater number of observation points and a known inter-electrode distance, which allow studying the propagation path and speed of electrophysiological signals. However, tissue oxygenation may be greatly impaired during MEA recording, requiring a high perfusion rate, which comes at the cost of decreased signal-to-noise ratio and higher oscillations in the experimental temperature. Electrical stimulation further stresses the brain tissue, making it difficult to pursue prolonged recording/stimulation epochs. Moreover, electrical modulation of brain slice activity needs to target specific structures/pathways within the brain slice, requiring that electrode mapping be easily and quickly performed live during the experiment. Here, we illustrate how to perform the recording and electrical modulation of 4-aminopyridine (4AP)-induced epileptiform activity in rodent brain slices using planar MEAs. We show that the brain tissue obtained from mice outperforms rat brain tissue and is thus better suited for MEA experiments. This protocol guarantees the generation and maintenance of a stable epileptiform pattern that faithfully reproduces the electrophysiological features observed with conventional field potential recording, persists for several hours, and outlasts sustained electrical stimulation for prolonged epochs. Tissue viability throughout the experiment is achieved thanks to the use of a small-volume custom recording chamber allowing for laminar flow and quick solution exchange even at low (1 mL/min) perfusion rates. Quick MEA mapping for real-time monitoring and selection of stimulating electrodes is performed by a custom graphic user interface (GUI).

We illustrate how to perform recording and electrical modulation of 4-aminopyridine-induced epileptiform activity in rodent brain slices using microelectrode arrays. A custom recording chamber maintains tissue viability throughout prolonged experimental sessions. Live electrode mapping and selection of stimulating pairs are performed by a custom graphical user interface.

鼠脑切片偶联微电极阵列癫痫活性的记录与调控

Temporal lobe epilepsy (TLE) is the most common partial complex epileptic syndrome and the least responsive to medications. Deep brain stimulation (DBS) ...

Noninvasive EEG Recordings from Freely Moving Piglets

The method allows the recording of high-quality electroencephalograms (EEGs) from freely moving piglets directly in the pigpen. We use a one-channel telemetric electroencephalography system in combination with standard self-adhesive hydrogel electrodes. The piglets are calmed down without the use of sedatives. After their release into the pigpen, the piglets behave normally&#8212;they drink and sleep in the same cycle as their siblings. Their sleep phases are used for the EEG recordings.

Here, we present a protocol to record telemetric electroencephalograms (EEGs) from freely moving piglets directly in the pigpen without the use of a sedative, making it possible to record typical EEG patterns during non-REM sleep, like spindle bursts.

自由移动仔猪的无创脑电图记录

The method allows the recording of high-quality electroencephalograms (EEGs) from freely moving piglets directly in the pigpen. We use a one-channel ...

Topographical EEG Recordings of Visual Evoked Potentials in Mice using Multichannel Thin-film Electrodes

Visual evoked potentials (VEP) allow the characterization of visual function in preclinical mouse models. Various methods exist to measure VEPs in mice, from non-invasive EEG, subcutaneous single-electrodes, and ECoG to fully invasive intracortical multichannel visual cortex recordings. It can be useful to acquire a global, topographical EEG-level characterization of visual responses previous to local intracortical microelectrode measurements in acute experimental settings. For example, one use case is&#160;to assess global cross-modal changes in VEP topography in deafness models before studying its effects on a local intracortical level. Multichannel epicranial EEG is a robust method to acquire such an overview measure of cortical visual activity.&#160;Multichannel epicranial EEG provides comparable results through a standardized, consistent approach to, for example, identify cross-modal, pathological, or age-related changes in cortical visual function. The current study presents a method to obtain the topographical distribution of flash-evoked VEPs with a 32-channel thin-film EEG electrode array in anesthetized mice. Combined with analysis in the time and frequency domain, this approach allows fast characterization and screening of the topography and basic visual properties of mouse cortical visual function, which can be combined with various acute experimental settings.

The present protocol describes a simple procedure to acquire and analyze the topography of epicranial visual evoked potentials with 32-multichannel thin-film electrodes in the mouse.

Visual evoked potentials (VEP) allow the characterization of visual function in preclinical mouse models. Various methods exist to measure VEPs in mice, ...

小鼠运动皮层中的细胞外电生理记录

Multichannel Extracellular Recording in Freely Moving Mice

The protocol aims to uncover the properties of neuronal firing and network local field potentials (LFPs) in behaving mice carrying out specific tasks by correlating the electrophysiological signals with spontaneous and/or specific behavior. This technique represents a valuable tool in studying the neuronal network activity underlying these behaviors. The article provides a detailed and complete procedure for electrode implantation and consequent extracellular recording in free-moving conscious mice. The study includes a detailed method for implanting the microelectrode arrays, capturing the LFP and neuronal spiking signals in the motor cortex (MC) using a multichannel system, and the subsequent offline data analysis. The advantage of multichannel recording in conscious animals is that a greater number of spiking neurons and neuronal subtypes can be obtained and compared, which allows the evaluation of the relationship between a specific behavior and the associated electrophysiological signals. Notably, the multichannel extracellular recording technique and the data analysis procedure described in the present study can be applied to other brain areas when conducting experiments in behaving mice.

作者聚焦:用于研究自由移动小鼠神经元活动的多通道细胞外记录的进展 

The protocol describes the methodology of extracellular recording in the motor cortex (MC) to reveal extracellular electrophysiological properties in freely moving conscious mice, as well as the data analysis of local field potentials (LFPs) and spikes, which is useful for evaluating the network neural activity underlying behaviors of interest.

自由移动小鼠的多通道细胞外记录

The protocol aims to uncover the properties of neuronal firing and network local field potentials (LFPs) in behaving mice carrying out specific tasks by ...

高密度的自由移动，使用基于聚酰亚胺的微电极的小鼠的脑电图记录

摘要

探索更多视频

此视频中的章节

设计和超轻重量的制作，可调节的多电极电生理记录小鼠探头

注射器-注射用网状电子技术稳定的慢性啮齿动物电生理学

神经元电活动外记录与神经活性物质在清醒小鼠微电泳应用相结合

高密度的脑电图采集在啮齿动物模型使用低成本的开源资源

干无创多通道头皮脑电传感器在小鼠视觉诱发电位记录中的应用

鼠脑切片偶联微电极阵列癫痫活性的记录与调控

自由移动仔猪的无创脑电图记录

Topographical EEG Recordings of Visual Evoked Potentials in Mice using Multichannel Thin-film Electrodes

自由移动小鼠的多通道细胞外记录

自由移动小鼠的多通道细胞外记录