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المحسن انخفاض التمثيل بيسلفيت تسلسل لتقييم الحامض النووي في قرار قاعدة زوج

Enhanced Reduced Representation Bisulfite Sequencing is a method for the preparation of sequencing libraries for DNA methylation analysis based on restriction enzyme digestion combined with cytosine bisulfite conversion. This protocol requires 50 ng of starting material and yields base pair resolution data at GC-rich genomic regions.

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Chapters in this video

0:05

Title

2:22

Adapter Ligation and DNA Size-selection via Gel Purification

7:32

Bisulfite Conversion, Enrichment PCR, and DNA Purification

9:40

Library Preparation for Sequencing

10:42

Results: ERRBS Yields Base Pair Resolution CpG Methylation Data

12:15

Conclusion

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